Hmces (NM_001025047) Rat Untagged Clone
CAT＃: RN207066
LOC500251 (untagged ORF) - Rat hypothetical protein LOC500251 (LOC500251), (10 ug)
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207066 representing NM_001025047
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGGGCGAACGTCCTGTCACTTGCCTAGAGATGCTCTCACTAGAGCCTGTGCCTATCTGGATCGGC AGGGCAGGCGTCAGCTCCCGCAGTGGAGGGACCCTGATAAGTACTGCCCCTCCTACAACAAGAGCCCGCA GTCTAGCAGCCCAGTGCTTCTCTCCAGACTGCACTTTGAAAAGGATGCAGATTCATCAGATCGGATAATT TTTCCCATGCGATGGGGCTTAGTCCCATCTTGGTTCAAAGAAAGTGATCCTTCCAAGCTGCAGTTCAACA CTTCCAACTGCCGTAGTGATACCATAATGGAGAAGCAGTCGTTCAAGGCTCCTCTGGGGAAAGGACGGCG GTGTGTTGTCTTAGCAGATGGATTCTACGAGTGGCAGCGATGTCAGGGAACAAACCAGAGGCAGCCGTAC TTCATCTATTTTCCTCAAAGCAAAACAGAGAAGTCAGGTGAGAACAGTGGTTCAGACAGCCTTAACAACA AGGAAGAGGTCTGGGACAACTGGAGGCTGCTGACAATGGCAGGGATCTTTGACTGTTGGGAACCCCCAAA GGGAGAGCGCCTGTATTCCTACAGCATCATCACTGTGGATTCCTGCAGAGGTCTGAGTGACATCCACAGC AGGATGCCTGCCATACTGGATGGAGAAGAGGCGGTCTCCAAATGGCTTGACTTTGGTGAGGTCTCTACTC AGGAAGCTCTGAAGCTAATCCACCCCATAGATAACATCACCTTCCATCCAGTTTCTCCAGTGGTGAACAA TTCCCGAAACAACACTCCTGAGTGTCTGGCTCCTGCTGACTTGCTGGTTAAGAAGGAGCCCAAGGCAAGT GGCAGTAGTCAAAGGATGATGCAATGGCTGGCTACAAAGTCACCCAAAAAGGAAGTCCCTGACTCACCCA AAAAGGATGCATCAGGTCTACCCCAGTGGTCCAGCCAGTTTCTACAGAAGAGCCCATTGCCGACTAAAAG AGGTGCCAGCAGCAGCCTCCTGGATCGATGGCTGAAGCAGGAGAAGGAGGATGAGCCTGTGGCCAAGAGG CCAAACAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025047 |
| Insert Size | 1062 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025047.1, NP_001020218.1 |
| RefSeq Size | 1486 bp |
| RefSeq ORF | 1062 bp |
| Locus ID | 500251 |
| UniProt ID | Q5XIJ1 |
| Gene Summary | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA. The HMCES DNA-protein cross-link is then degraded by the proteasome. Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (By similarity). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells. May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207066 | LOC500251 (Myc-DDK-tagged ORF) - Rat hypothetical protein LOC500251 (LOC500251), (10 ug) |
CNY 3990.00 |
|
| RR207066L3 | Lenti ORF clone of LOC500251 (Myc-DDK-tagged ORF) - Rat hypothetical protein LOC500251 (LOC500251), (10 ug) |
CNY 6080.00 |
|
| RR207066L4 | Lenti ORF clone of LOC500251 (mGFP-tagged ORF) - Rat hypothetical protein LOC500251 (LOC500251), (10 ug) |
CNY 6650.00 |
