Spdl1 (NM_001034138) Rat Untagged Clone
CAT＃: RN205913
Spdl1 (untagged ORF) - Rat coiled-coil domain containing 99 (Ccdc99), (10 ug)
CNY 7700.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ccdc99; RGD1306908 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205913 representing NM_001034138
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGATATCACAAATCTTCGAAACAAGCTCAAAGAGTGTGAAGACGAGCGACTGAAAGCTGCAC AGTATGGCCTACAGCTATTGGAGAGGCAGACGGAGTTGCAGAGTCAGCTGGATAAATGTCATGAAGAAAT GATGACCACAGCTGAGAATTATAACCAAGAGAAGTATGCCCTTCAAAGAGAAGTAGAGCTCAAGAGTCGG ATGTTGGAGAGTCTGAGCTGTGAGTGTGAGGCCCTGAGGCAGCAGCAGAAAGCGCAGCTGGAGCAGTTAG AAATGCAGCTGCACAGAAGCCACCGGCAGGAAGTGCACGGCCTGAGAAACAAGTTAGAGAATCTGAAAGT GGAATTAGACGAAGCAAGGCTTAGTGAGAAGCAGCTGAAGCAGAAGCTGGATCACCAGGGGGAGCTTCTC TCTCACAAGTCGGAAGAGCTCCGGCTGCTGTCTGAGCAACGGGCACTCAGCAGTGTGTCGTCAGAGCTGC TGTCTCTTCAGACCGAGCTGACCGAGGCAGAGGGTGTGAAGAACGCCCTCAGAGAAGAGGTGAACGAACT ACAGTGCAAACAGGAGCAACTAGAATGTCTCAACGCTTCCTTACTGCACCAGGTAGACCGGCTTAAAGAA GAAAAGGAGGAGCGAGAGAAGGAGGCGGTCTCTTACTATAATGCCTTGGAGAAAGCTCGTGTAGAAAATC AGGATCTCCAGGTGCAGCTGGGCCATGCACTCCAGCAGGCGGCGGATCCCAACAGCAAAGGAAACTCTCT GTTCGCAGAGGTGGAGGATCGAAGGGTGGCAATGGAACGCCGGCTCAACCTGATGAAAGACAAATACCAG TCACTGAAGAAGCAAAATGCCTTCACCAGAGATCAGATGAACAAGATGAAGTTACAAATTTCCACGTTGC TGAGGATGAGGGGATCACAAACCGAATTTGAGCAGCAGGAACGGTTGTTCGCCATGTTAGAGCAGAAGAA TGGTGAAATAAAACACCTTTTAGGGGAAATTAATAAACTGGAGAAATTTAAGAACTTATATGAAAATATG GAATCCAAGTCTTCCACATCAGACAGTGCTTGTGCTCTAGAAGACAGCACCTACTACACAGATTTGCTTC AGCTGAAGCTTGATAAACTAAACAAAGAAAATGAAAGCACGAAGGGTGAACTGTCCATCCAGAGGATGAA GGCATTATTTGAGAGCCAGCGGACCCTGGATATTGAACGGAAACTGTTTGCAAATGAAAGACACCTGCAG CTCTCAGAGAGTGAAAACCTGAAACTGAGAGCTAAGCTAGATGAACTCAAGCTGAAGTACGAGCCTGAAG AGAGAATTGAAGTACCTGTACTCAAAAGGAGGCGTGAGGTGCTGCCATTGAATATAAACACCCCAGAAGA AACAGCTGCTGCCAGTGCCACTGGGGAAGACGTTTCTAGACTTCCACTTGAGAGGAAGGAGGAGTCCTGT CTTGATAACTTAAAAGATAACACGGTGCAGTGGAAGCAGCCAGCCAGCCTCTCTCCTCATAAGAGCCTGC CTCTGGATACACAGCCGAAGAAGGAGAAGTGTGTGACGCTTGTGGCTAGTCCAGCCAGCACTGAGGTCCT ACATGAACAAAGTGGGAACACCCCCAGTTCTCCCAGGTTAACTGAAGAATCAAGGCTTCCAACAAAAGTG AAGGAAAGAAAAGAAGCTACAAGCAAATTGGAAAAAGGAGCTTCTAAGAAATCACACACCATCATGTATG TGTCCTCGAAGTCAACCCCAGAGATGCAGTGCCCACAGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001034138 |
| Insert Size | 1794 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001034138.1, NP_001029310.1 |
| RefSeq Size | 2310 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 303037 |
| UniProt ID | Q3KR99 |
| Gene Summary | Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205913 | Spdl1 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 99 (Ccdc99), (10 ug) |
CNY 7700.00 |
|
| RR205913L3 | Lenti ORF clone of Spdl1 (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 99 (Ccdc99), (10 ug) |
CNY 9500.00 |
|
| RR205913L4 | Lenti ORF clone of Spdl1 (mGFP-tagged ORF) - Rat coiled-coil domain containing 99 (Ccdc99), (10 ug) |
CNY 10450.00 |
