Spef2 (NM_022620) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Kpl2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205007 representing NM_022620 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAGATCCTGTGCCAGTGGCTCAACCAGGAGTTGAAGGTGTCTCGGACCGTGAGTCCCAAGTCAT TTGCAAAGACATTTTCTAATGGCTATCTAATTGGAGAAGTTCTGTTCAAGTTTGAACTTCAGAGTGATTT TTCAGAGTTTTCAGAGAGCAGGGTCTCAACTGCCAAACTTAATAATTTCTCCCGCTTGCAACCAACGCTT CACCTGTTGGGTGTGCAGTTTGATCAGAATGTGGCTCAGAGCATCATCACAGAAAAGCCCGGGGCAGCAA CCAAACTTCTGTATCAGTTGTACATTGCTCTTCAGAAAAAGAAGAAAACTGGGCTGACTGGATTAGAGAT ACAAACCATGCAACCCCAAACAAACCAGCGGCTTCAGGCCCTCAAGAGTGAGGCTTTTCGAGAGCAACGC TTAAACAGAAGACGACAAAATGAGATAATGGCCAAAATCCAGGCAGCCATTATACAGATTCCCAAGCCTG AACCCAACCGTACCTTGAAAGCTATTGAGGCTCAAAAACTGATGAAAAAGAAAAAGGAGGCAGAGGACGT GGCCAATGAGATTAAGAAATTTGAAGCACTAATTAAAAAGGATCTCCAAACTAGAGAAAGTATATCCAAG ACTTCTTTAGATACAATAGACCAGACAGCTGCGGAACTGCTCAACACTTACTCAGATGACGACTACATTA AAAAGATCCAGAAACGCCTAGAAGAAGATGCTTTTGCACGTGAACAAAGGGAAAAAAGACGACGGAGGTT GTTGATGGACCAGCTGATAGCCCATGAAGCACAGGAGGAGGCCTATCGGGAAGAGCAGCTCATTCACCGG CTCATGCGCCAGTCCCAGCAGGAGCGCAGGATTGCTGTGCAGCTCATGCACGTTCGCCACGAGAAGGAAG TTTTGTGGCAAAATAGAATTTTCAGAGAGAAACAATTTGAAGAAAGACGACTTAAGGATTTCCAGGATGC TCTAGATAGAGAAGCGGCTCTCACCAAACAAGCCAAGATTGATTTTGCAGAGCAAACCTTGAGGGCGAAG GAAATTCATGAAAAGATCGCCATGGAAAGAGCCCAACAGCGTTACGAGAAGCATTATGGGATATGTGCAG AGATTTTGGATCAAATACTTGATTTGTGCACGAAGGTAGCAGACTATAGGTTGCTGACAAATAATCTTAT TCCACACAAGATGATGCGTGATTGGAAAGAACTGTTTTTTAGTGGAAATCCCATATATGAACAGACTTCT CATACACATGGACAAACAGAATCCACTGAGGATCACCATGTAGAGTTGAACAAAAGGAACTTACTCGATA GCAAAGATTATGAAGACTATAAGAACATGACTGGGGAGTGGGCCTTACCAGAGGACATGGCAAACAGTTC ACCACCCTCCAACAATAACATACTGGGTCATGTGCTTCTGAGATTAATTGAAAACTCGTATCCGTCTGAG AAAGAAGTTGCAGACACTGAATTACCTTCATTTGCTGTGAAGGGTTGTTTACTGGGGAAGACACTCAGTG GAAAAACTACAATTCTCAAGGCTCTTCAAAATGACATCCCTATTCAAGTACTTTCTATTGATACTCTTGT CCAAGAGGCTATTCAGGCATTTAATGATGGAGAAAAAAGTAGTAAGGCACCATCGATGCATGAAGATGTT AAAGGAGATCCTGAAGTGGACCAGAAAGAAATGATCATACCTCAGGATAAAAATGAGCTAACAGATACTC AAGTTCCAGGGGAAGCAGCACCCCAAAAAGAAGGTACGAAGAGTAGTGACTTTGAACAATTCAGACCAAG TAATAGCTTTCTGTCACTTAGCATGCGAGCTCAACTTGGTGCAAAATCAGAACTGATGCTCAGAAGAGGC AAGAGCGTTCCAGATGTACTGCTGGTTAACATCCTGGTAAATGCTATTAAGAAGATACCTGTGAACCAAA GCTGGGTTCTAGATGGATTTCCAATGACAGTAAATCAAGCCAAGCTCCTGGAGGAAGCTCTTACAGGATA CAAAAGAAAATCCCTGCAGTTAAAGAAGAAAAAAGCACAAATGCCCACATTGGCCTTGAGCCCCACAACT TCCAAAGAGGTCCCTTTTCTCCCATCTGCGTTCGATTTTGCCATATTGCTTGATATTTCAGATAACTCAT CACTGGCTCGCATTAACGATATCATAGCTAAAGAAATCTCACATGAAATTTCTCATGAAAATGTTAGCGG TCCTACAATTACTTCAAGTGAAGAAAAGAGTGAGGACCGGAACTTAAGAGACCACATACAGCACAGAATT GTTGGCTTCTTGGACAACTGGCCATTGTTGGAAGAGTGGTTTACAGAGCCAAGAAACATTTTGAAAAAAG TCAATGCTGAAATAGATGAAGCGTTCTTATGCCAAAAAATAAAAGAAATTTTTGCTACTGAAGTATCAAA TAAAAAAATCAAAGTTGAAAAGAAACTAGAAGAAAAGGAAACTGAGAAAAAGTCAGCAGTTTCTCCGACT GAGCCTCCTACGCCGACCCCTCCCCTTGCTTCTGAAGCAGAAAAAGAAAAGGAGGTTCATCAGGCGAAAC CTTCTGGGAAAGGAAAGACACAATCAGTTTCTCCTAAAGGAAAAGCACAAGGAGGCAAAATTTCCGTAAA GAAATCACCCATTGACTCAACAGAAGTATCACCCACACTAATAGCAATGCCGACAACTAAGCCAGGATCA GAAGAATGGGTCTATGTGACTGAGCCTATACCTGAGGAGCTACCTTCGTTTTTAGTACCTTACTGGGAAC TGATAGAGAAAACCTACATAAGCCACATCAAAACAGTGCTTCGACAGCTCAGAGAAAGCCAACACACCGT GCTCTCTTATTTATATGAGACTAGGACAAGTTTTCAGGAGTTTCTAAGGCGTCCAGATCACAAGCAAGAT TTTGTATCTCAGTGGCAGGCTGATTTCAACTCAGTTCCTGAAGACCTGTGGGAAGATGAGGAAACAAAGG CTGAACTACACCAAAGAGTTAATGATCTGCGAGACCGCCTGTGGGACATCTGTGAAGCGAGGAAGGAAGA GGCAGAGCAGGAGAGACTTGATATCATTAACGAGAGCTGGCTGCAGGACTATATCGGAATAACAATGAAT CACTTCTTTTCCCTGATGCAGGCAGAAGTGAACCGTTTCCAAGATACCAAAAGACTCCTTCAAGATTATT ACAGAGCAATGGAATCCAGAATCCCACTGGAAGACAATAAGAAATTTACTCGAATCCCATTGGTTCAACT CGATGGTAAAGAAATTTCAGAACACCAGCTCAGAATTCCTCTAGTTCCTCGAATATCCAGTTCTCCAGAA AATGTTAGCATGAGACCAAAGGTGAAAGCATTACTGAAGGGCAGATTCGATCCACCACTGGAAAGTCTGG AAGCCAACTTTGAAGGAGATGAGAAGATAGTATTAGACACCTGGCAGCAGGCTTCCTTAGCCATCTCCCA AATGGTGGCTGCTGAAGTTCATCAAAGGCTCGCAGAGGAAGAAAAGGAACAACCCCAACTAGATCCCAAA GAAAAGTCTCCTCAGTCGGGTACAAACAAAAAGGCCAAGAAGGAGAAAGAACAGGCCAAGAAGGAGAAAG AACAGGCCAAAAAGGAGAAAGAACAGGCCAAGAAGGAGAAAGAACCGCCCAAGAAGAAAATGGCAGAAAA GAAAGGAAAAGGTAAATCATCCCCTGTGGTAGAAGTCAGTCCCGTCACAATAACGCCCGAGGAAATGGCT GAAATGGAAAGGAAGAATGAGCTGAAGCTCAAGATAAAGGAAGAACATCTTGCCGCCCTGCAGACAGAAG AACAAGCCACACAATTTCGACTTGAACTAATAAAGTCAAAAGCTTTGTCCATCCTGGAAGATTTAGTAAC AAAGGTGATTGAGGTTTACAGGTTCATGGAAAAATGGCTTGGTAAGAGATATTTGAATGAAATGGCCAGT ATACAGAAATTGACCGAAGTAGCTCGCTACCACATTGAGACAGCTACGAAAATTCAAAATGAGATTTATT TAAGCCAAGAAGACTTCTACATCAATGGAGACATAAAAGTCTTCCCTGACCCTCCTCCACCAACCCGCCC ACCACCGGTAGAAAAGGAAGAAAATGGCACCCTAACAATCGAACAGCTTGATAACCTTCGAGATCAGTTC TTAGATATGGCGCCTAAAGGCATAATAGGAAATAAAGCTTTCACTGACATTCTGCTTGATCTGGTTACCC TGAACCTGGGAACAAACAACTTGTCTAGCAGCTGGATGCACCTCACCCAACTGGATTTGCAGGAGATAAC ATCTTTATTAACAGTCAACACAGAGTTCGTGGACTGGAGGAAGTTCTTGATGGTAGCAGCCATGCCATGG CCCATCCCCCTGGAGGAGGAGCTCCTGGAGACCCTGCAGAGGTTTAAGGCCACCGATGAAGCTCAGACAG GCACCATCACCTTTGAACAATATAAGCAGGCTGGTCTATGGTTTTCAGGAGATGAAGATATAAAAATTCC AGAAAATCCCCTTGAACCCTTGCCATTCAATAGACAGGAGCATCTCATAGAGTTCTTCTTCAGGCTGTTT GCTGACTCTGAGAAGGATCCACCTCAGCTTGACTACACTCAGATGTTGCTGTATTTTGCCTGCCACCCAG ACACTTTGGAAGGAGTCTACAGGGCCCTCAGTGTGGCTGTGGGGACTCACATATTCCGGCAGGTTGAAAC TCCAATGCTAACTGCAGAAAAGACCTCCATCTCCTCTGTGAGTCCCAATGAGGAATTCCCTGAAGCTGAA GAAAACAGTACAAGAGAGGAGCTGAAGGAGGAGAAAGATGAGAGGGACCAAAAGGAAGAAGAAATCCCAG AAAATGCAAACACTGAAAAGATTTCCATGGAAACACTGCTCAAAGTGTTTGGAGGAGGAAATGAAGTGCT GGACGCTAACAGATTTGCCAGCTACCTAAAGACAGAGAATATTTACTCAGAGAACCTCATCAAGACGTTC CAGGACCTGGGTGCCAAGAACCTAGAGCCAATCGAAGTTAATATTCTCTTGAAGCATCCTTACATTCAGG ACCTGATTACGAATTATGTAGACTATAAAATCCCTGACATTAAAATGATCCTTCAAAGGAGTGAGCATGC ACAAGGCAGTGATGGAGAAAGGTCACCCTCAAGACTTACAGACGAAAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022620
Insert Size	5235 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022620.1, NP_072142.1
RefSeq Size	5365 bp
RefSeq ORF	5235 bp
Locus ID	64555
UniProt ID	Q9R095
Gene Summary	may play a role in differentiation or function of ciliated cells in the airway [RGD, Feb 2006]

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