Ffar4 (NM_001047088) Rat Untagged Clone

CAT#: RN202773

Ffar4 (untagged ORF) - Rat G protein-coupled receptor 120 (Gpr120), (10 ug)



  "NM_001047088" in other vectors (3)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Gpr120; O3far1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN202773 representing NM_001047088
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCTGAGTGTGCGCAGACGACGGGCCCTGGCCCCTCGCGCACCCCGGACCAAGTCAATCGCACCC
ACTTCCCTTTCTTCTCGGATGTCAAGGGCGACCACCGGCTGGTGCTGAGCGTCCTGGAGACCACCGTTCT
GGGACTCATCTTTGTGGTCTCACTGCTGGGCAACGTGTGTGCCCTGGTGCTGGTGGTGCGCCGTCGGCGC
CGTGGGGCGACAGTCAGCTTGGTGCTCAACCTCTTCTGCGCGGATTTGCTCTTCACCAGCGCCATCCCTC
TAGTGCTCGTGGTGCGCTGGACTGAAGCCTGGCTGCTGGGGCCCGTCGTCTGCCACCTGCTCTTCTACGT
GATGACCATGAGCGGCAGCGTCACGATCCTCACGCTGGCCGCGGTCAGCCTGGAGCGCATGGTGTGCATC
GTGCGCCTGCGGCGCGGCTTGAGCGGCCCGGGGCGGCGGACTCAGGCGGCGCTGCTGGCTTTCATATGGG
GTTACTCGGCGCTCGCCGCGCTGCCCCTCTGCATCTTGTTCCGCGTGGTCCCGCAGCGCCTTCCCGGCGG
GGACCAGGAAATTCCGATCTGCACATTGGATTGGCCCAACCGCATAGGAGAAATCTCATGGGATGTGTTT
TTTGTGACTTTGAACTTCCTGGTACCAGGACTGGTCATTGTGATCAGCTACTCCAAGATTTTACAGATCA
CGAAAGCCTCGCGGAAGAGGCTTACGCTGAGCTTGGCATACTCCGAGAGCCACCAGATCCGAGTGTCCCA
GCAGGACTACCGGCTCTTCCGAACGCTCTTCCTGCTCATGGTTTCCTTCTTCATCATGTGGAGTCCCATC
ATCATCACCATCCTCCTCATCTTGATCCAGAACTTCCGGCAGGACCTGGTTATCTGGCCGTCCCTTTTCT
TCTGGGTGGTGGCCTTCACGTTTGCCAACTCCGCCCTAAACCCCATTCTGTACAACATGTCGCTGTTCAG
GAGCGAGTGGAGGAAGATTTTTTGCTGCTTCTTTTTCCCAGAGAAGGGAGCCATTTTTACAGAAACGTCT
ATCAGGCGAAATGACTTGTCTGTTATTTCCACCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001047088
Insert Size 1086 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001047088.1, NP_001040553.1
RefSeq Size 1388 bp
RefSeq ORF 1086 bp
Locus ID 294075
UniProt ID Q2AC31
Gene Summary Receptor for medium and long-chain free fatty acids (FFAs). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects, particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1-dependent effect, but independent of the G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Mediates FFA-induced inhibition of apoptosis in enteroendocrine cells. May play a role in the regulation of adipocyte development and differentiation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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