Spc25 (NM_001009654) Rat Untagged Clone
CAT＃: RN202582
Spc25 (untagged ORF) - Rat SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc25), (10 ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1307811; Spbc25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202582 representing NM_001009654
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAAGACGAACTGGCAGCCTTCGAGAAAAGCATAAACGAATTTGGGGACAAGTTCCGGTACAGAC TCAGTGATAATCGTAGCCAGGTGCTGGGACTTAAAGATGCCTTCAAGGACTCCATTAGAGCGCTTTCAGA AAAGCTGTCTTTGAAACTAAAGGAAGAAGAAAGGATGGCTGAGATGATTCTGGAATATAAAAATCAGCTC TGTAAGCAGAATAAGCTCATTCAAGAAAAGAAGGATAATGTGTTAAAGATGATTGCTGAAGTGAAAGGCA AAGGGCAGGAGGTGGAAGTGCTGACGGCAAACATCCAGGATCTGAAGGAGGAGTACGCCAGGAAGAAGGA GACCATTTCCACTGCTAACAAAGCCAATGAAGAGAGATTGAAACGGCTGCAGAAATCAGCGGATTTGTAT AGAGATCGCCTTGGACTAGAAATTAGAAAGATTCCTGGTGATAAGCTGCAGTTTATATTTACTAGTATTG ACCCTAAGAATCCTGAGAGCCCCTTCATGTTTTCCCTGAGCATAAATGAAGCTAAGGAATATGAAGTGTC CGACTGTTCACCTCATCTTGAGTGCCTAGCAGAATTTCAGGAGAAAGTCAGGAAGACCAACAATTTTTCA GCCTTCCTTGCCAATGTTCGGAAAGCTTTTATAGCTAAGGTTCATAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001009654 |
| Insert Size | 681 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001009654.1, NP_001009654.1 |
| RefSeq Size | 1291 bp |
| RefSeq ORF | 681 bp |
| Locus ID | 295661 |
| UniProt ID | Q5M856 |
| Gene Summary | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202582 | Spc25 (Myc-DDK-tagged ORF) - Rat SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc25), (10 ug) |
CNY 3990.00 |
|
| RR202582L3 | Lenti ORF clone of Spc25 (Myc-DDK-tagged ORF) - Rat SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc25), (10 ug) |
CNY 6080.00 |
|
| RR202582L4 | Lenti ORF clone of Spc25 (mGFP-tagged ORF) - Rat SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc25), (10 ug) |
CNY 6650.00 |
