Tnks (NM_001106084) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202383 representing NM_001106084 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTCGCGTCGCTCTCAGCATCATCACCACCATCATCAGCAACAGCTCCAGCCCGCCCCAGGGG CTTCGGCTCCGCCACCGCCACCTCCCCCGCCGCTCAGCCCGGGCTTGGCCCCGGGACCTACCCCGGCCTC TCCCACGGCCGGCGGCCTGGCGCCCTTCGCCTCCCCGCGGCACGGCCTGGCGCTGCCGGAGGGGGATGGC AGCCGGGACCCGCCCGACAGGCCGCGATCTCCGGACCCGGTGGACGGCGCGGTCTGCACGGTGGCTGCTA CGGCCGCGGCCCCGGCAGCTTCCGCCGCCGTCGGGGTCGCTCCCAGCCCAGCCGGCGGCGGCGGTAACAA CTCCGCGTCGTCCACTTCTTCGCCTACTTCTTCTTCGTCTTCCTCTCCGTCCTCCCCTGGCTCGAGCATA GCGGAGAGTCCCGAAGCCGCCGGAGTTGGCTCCACAGCAACTTTGGGACCCGGGGCGGCGGGCCTTGGCC CGGGGGTCCCAGCCGTGAGTGGGGCCCTGCGGGAGCTGCTGGAGGCTTGTCGAAATGGGGACGTGTCCCG GGTGAAGAGGTTGGTGGACGCAGCGAACGTGAATGCAAAGGACATGGCCGGCCGAAAGTCTTCTCCCCTG CACTTCGCAGCAGGTTTTGGAAGGAAGGATGTTGTAGAACACTTGCTGCAGATGGGTGCCAATGTCCATG CCCGTGACGATGGGGGTCTCATCCCACTTCATAACGCCTGTTCTTTTGGCCATGCGGAGGTTGTGAGTCT GTTACTGTGTCAAGGAGCAGATCCGAATGCCAGAGATAACTGGAACTATACGCCTCTGCATGAAGCTGCC ATTAAAGGGAAGATCGACGTTTGCATTGTGCTGCTGCAGCACGGAGCTGATCCAAACATCCGGAACACCG ATGGGAAATCGGCTCTGGACCTGGCAGATCCTTCAGCAAAGGCTGTCCTCACAGGTGAATACAAAAAGGA CGAACTCCTAGAAGCTGCTAGGAGTGGTAATGAAGAAAAACTAATGGCTTTACTGACTCCTCTAAATGTG AATTGCCATGCCAGTGATGGGCGAAAGTCTACTCCGTTACACCTGGCCGCGGGCTACAACAGAGTGCGGA TAGTTCAGCTGCTGCTACAGCACGGAGCTGATGTCCACGCAAAAGACAAAGGCGGGCTTGTACCTCTTCA TAATGCATGCTCATACGGACACTATGAGGTCACAGAGCTGCTGTTAAAGCATGGCGCTTGTGTCAACGCT ATGGATCTGTGGCAGTTCACCCCGCTGCATGAAGCCGCTTCCAAGAACCGCGTCGAAGTCTGTTCTTTGT TACTTAGTCATGGTGCTGATCCAACCTTGGTCAATTGCCATGGCAAAAGTGCTGTGGATATGGCTCCCAC TCCTGAGCTCCGGGAAAGACTGACATATGAATTTAAAGGCCATTCTTTACTACAAGCTGCCAGAGAAGCA GACCTGGCCAAAGTGAAGAAAACACTTGCTTTGGAAATCATTAACTTCAAGCAGCCACAGTCTCATGAAA CTGCCTTGCATTGTGCTGTGGCCTCCTTGCATCCCAAACGAAAGCAGGTGGCAGAACTGCTACTTAGGAA GGGCGCCAATGTCAATGAAAAAAACAAGGACTTCATGACTCCTTTGCATGTTGCAGCTGAAAGAGCTCAT AATGACGTCATGGAGGTTCTGCATAAGCATGGAGCAAAGATGAACGCGCTGGACAGTCTAGGTCAGACTG CTTTGCACAGAGCTGCCTTGGCTGGCCACCTGCAGACCTGCCGCCTCCTGCTGAGTTACGGCTCTGACCC TTCCATCATCTCCCTGCAAGGCTTCACAGCCGCGCAGATGGGGAACGAAGCGGTGCAGCAGATTCTGAGC GAGAGTACTCCTATCCGCACTTCTGATGTGGACTATCGACTCTTAGAGGCATCGAAAGCTGGTGACTTGG AAACTGTGAAGCAACTGTGCAGCCCGCAGAATGTGAACTGCAGAGATCTGGAGGGCCGCCATTCCACTCC CTTACACTTCGCTGCAGGCTACAACCGTGTGTCTGTCGTCGAGTACCTTCTACATCATGGAGCTGATGTC CATGCCAAAGACAAAGGGGGCTTGGTTCCCCTTCACAATGCCTGCTCCTATGGACACTACGAGGTAGCTG AGCTCTTGGTGAGGCACGGGGCTTCTGTCAACGTGGCGGACTTGTGGAAATTTACCCCTCTCCATGAAGC CGCAGCCAAGGGAAAATATGAGATCTGCAAACTCCTTCTAAAACATGGAGCAGATCCAACTAAGAAGAAC AGAGATGGAAACACACCCCTAGATCTGGTCAAAGAAGGAGACACAGATATTCAGGACCTACTGAGGGGGG ATGCTGCCCTGTTGGATGCTGCCAAGAAGGGCTGCCTGGCCAGAGTGCAGAAGCTTTGTACTCCAGAGAA CATCAACTGCCGAGACACCCAGGGCAGAAACTCAACTCCTCTCCACCTGGCAGCGGGCTATAATAACCTG GAAGTAGCCGAGTACCTGCTAGAGCGCGGCGCTGATGTGAACGCCCAGGACAAAGGAGGTTTAATTCCAC TCCACAACGCAGCGTCTTATGGGCATGTTGATATAGCAGCATTACTGATAAAGTACAACACGTGTGTAAA CGCAACGGATAAGTGGGCCTTCACTCCCCTCCACGAAGCAGCCCAGAAGGGGAGGACACAGTTGTGTGCC CTACTCCTAGCACATGGCGCAGACCCAACCATGAAGAACCAGGAGGGTCAGACACCTTTAGACCTGGCAA CGGCTGATGACATCCGAGCTTTGCTGATAGATGCCATGCCCCCTGAGGCCTTACCTACCTGTTTTAAACC TCAGGCAACTGTAGTTAGTGCCTCTCTGATCTCACCAGCGTCCACCCCGTCCTGCCTCTCTGCTGCCAGC AGCATAGATAACCTCACTGGCCCGTTAGCAGAGCTGGCTGTAGGAGGAGCCTCCAATGCGGGGGATGGTG CTGCGGGTGCAGAAAGGAAGGAAGGAGAAGTTGCGGGTCTTGACATGAATATCAGCCAATTCCTTAAAAG CCTTGGCCTTGAGCACCTTCGGGACATCTTTGAAACAGAGCAGATCACGTTAGATGTGCTGGCCGATATG GGTCATGAGGAACTGAAAGAAATAGGCATCAATGCCTATGGACACCGCCACAAGTTAATCAAGGGCGTTG AAAGACTCTTAGGTGGACAACAAGGAACCAATCCGTATCTGACCTTTCACTGTGTGAATCAGGGAACTAT TTTACTAGATCTTGCTCCAGAAGATAAAGAATACCAATCAGTAGAAGAAGAGATGCAGAGTACTATTCGG GAGCACAGAGATGGCGGGAATGCTGGTGGCATCTTCAACAGATACAATGTCATTCGAATTCAGAAAGTTG TAAACAAGAAGCTGAGGGAGCGGTTTTGTCACAGACAAAAGGAGGTGTCTGAGGAGAACCACAACCATCA CAATGAGCGCATGCTGTTCCACGGTTCCCCTTTCATTAATGCCATTATTCATAAAGGGTTTGATGAGCGG CACGCATACATTGGAGGGATGTTTGGAGCTGGGATTTACTTTGCCGAAAACTCTTCCAAAAGCAATCAGT ATGTTTATGGAATTGGAGGAGGAACAGGCTGCCCCACACACAAAGATAGGTCATGTTACGTATGTCACAG ACAAATGCTTTTCTGTAGAGTGACCCTTGGAAAGTCTTTTCTGCAATTCAGCACCATGAAAATGGCCCAT GCGCCTCCAGGACATCACTCAGTCATCGGTAGACCAAGTGTCAATGGGCTGGCGTATGCTGAATATGTCA TCTACAGAGGGGAACAGGCATATCCAGAATATCTCATCACCTACCAGATCATGAAGCCGGAAGCCCCTTC CCAGACTGCAACGGCAGCAGAGCAGAAGACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106084
Insert Size	3954 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106084.2, NP_001099554.2
RefSeq Size	4575 bp
RefSeq ORF	3954 bp
Locus ID	290794

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