Robo3 (NM_001108135) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202319 representing NM_001108135 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGCATCGTGGAGCAGCCGCCAGATCTGCTCGTTTCCAGGGGCGAGCCGGCCACTCTGCCCTGCC GTGCTGAAGGCCGGCCTCGACCCAACATCGAGTGGTACAAGAATGGGGCGCGCGTGGCCACTGCACGCGA GGATCCGCGGGCTCACCGCCTGCTGCTGCCCAGCGGCGCCCTCTTCTTTCCCCGCATCGTGCACGGGCGT CGCTCTCGGCCTGACGAGGGTGTCTACACCTGTGTGGCTCGCAACTACCTGGGAGCAGCGGCTAGCAGAA ACGCCTCTCTGGAAGTAGCTGTCCTCCGAGACGATTTCCGGCAATCTCCTGGGAACGTGGTGGTAGCAGT GGGGGAGCCAGCAGTAATGGAATGCGTGCCCCCTAAGGGTCACCCAGAGCCTTTGGTGACCTGGAAGAAG GGCAATACAAAGCTTAAGGAAGAGGAGGGAAGGATCACCATACGTGGAGGAAAGCTGATGATGTCACATA CATTCAAGAGTGATGCTGGCATGTACATGTGTGTAGCCTCCAACATGGCTGGAGAACGAGAGAGTGGGGC AGCTGAACTTGTGGTACTGGAGCGTCCCTCATTTCTGCGTAGACCAATAAACCAGGTTGTCCTGGCTGAT GCCCCTGTGAATTTCCTGTGTGAGGTGCAGGGAGATCCCCAGCCCAATCTACGCTGGCGCAAGGATGATG GGGAGCTGCCCACTGGCAGGTATGAGATTCGGAGTGACCACAGCCTTTGGATCGAGCAAGTGAGTGCTGA AGACGAGGGGACTTACACCTGTGTGGCAGAAAACAGTGTGGGCCGAGCAGAGGCATCTGGCTCCCTCAGT GTTCACGTCCCACCACAGTTTGTGACTAAGCCCCAGAACCAGACAGCAGCTCCTGGAGAAAACGTGTCTT TCCAATGTGAGACCAAAGGAAACCCACCACCTGCCATCTTCTGGCAGAAGGAAGGGAGTCAAGTCTTGCT TTTCCCTAGTCAGTCACTCCAGCCCATGGGACGCCTCTTAGTCTCTCCAAGAGGCCAGCTCAACATCACT GAAGTAAAGATCGGGGATGGTGGCTACTATGTGTGCCAGGCTGTCAGTGTGGCTGGTAGCATCCTGGCTA AGGCCCTGTTAGAGATAAAAGGAGCCTCCATAGATGGACTACCTCCCATCATTCTCCAGGGACCAGCCAA TCAGACATTGGTACTTGGCTCTTCTGTGTGGCTGCCATGCAGAGTGATTGGAAACCCTCAGCCCAATGTC CAATGGAAGAAGGATGAGACGTGGCTGCAGGGGGATAACTCACAGTTCAACTTAATGGACAACGGCACAC TATACATTGCCAGTGTACAGGAGATGGACATGGGTTTCTACAGCTGTGTGGCCAAGAGTTCCATAGGGGA GGCCACATGGAATAGCTGGCTTAGGAAGCGAGAACATTGGGGAGCATCACCAGGTCCAGCAACAGAACCC GGTAATCCTCCAGGCCCTCCCTCTCAGCCAATAGTCACAGAGGTAACCACAAACAGCATTACCTTGACCT GGAAGCCCAATCCACAGTCTGGGGCCAGAGCTACCTCCTATGTGATAGAGGCCTTCAGCCAAGCAGCTGG CAACACGTGGCGGACGGTGGCAGATGGGGTACAGCTGGAAACGCACACCGTCAGCGGCCTGCAGCCCAAT ACCATCTACCTATTCCTCGTGAGAGCTGTTGGAGCCTGGGGCCTCAGTGAACCCAGTCCTGTCTCTGAGC CTGTTCAAACCCAGGACAGCAGCCTATCTAGGCCAGTGGAGGACCCGTGGAAAGGCCAGCAAGGACTGGC TGAAGTGGCCGTGCGAATGCAGGAGCCCATAGTCCTTGGACCCAGAACTCTGCAGGTGTCCTGGACTGTA GAGGGCCCTGGCCAGCTGGTTCAGGGATTCCGTGTGTCTTGGAGGATTGCAGGCCTTGACCAGGGAAGCT GGACAGCGCTAGACGTACAGAGTCCACACAAGCAAAGTACTGTGCTAAGAGGCCTCCCCCCAGGGTCCCA AATACAGATCAAGGTGCAAGTCCAAGGCCAGGAGGGGCTGGGGGCTGAAAGCCCCTCTGTGACCCGGAGC ATTCCTGAAGAGGCCCCCAGTGGCCCCCCTCAGGGAGTGGCTGTGGCCTTGGGGGGTGATCGCAACAGCA GTATCACTGTATCCTGGGAGCCTCCACTTCCCTCCCAGCAAAATGGGGTTATCACTGAATACCAGATCTG GTGCCTGGGTAATGAAAGCCGATTCCACCTCAATCGATCTGCAGCAGGCTGGGCACGCTCTGTGATGTTC CGGGGACTGCTACCGGGCCTGCTCTATAGAGCCATAGTGGCAGCAGCTACCAGCGCTGGCGTGGGCGTGG CCAGTGCCCCAGTGCTAGTGCAGCTGCCATTCCCTCCGGTTGCGGAGCCCGGGCCTGAGGTCAGCGAGGG GCTGGCGGAACGGTTGTCTAGGGTGCTGCGGAAGCCTGCTTTCCTGGCTGGCAGCAGCGCGGCCTGTGGG GCGCTGCTTCTCGGGCTCTGCGCCGCCCTCTATCGGCGCCAGAAGCAACGCAAAGAGCTCAGTCATTATA CCGCCTCCTTTGCCTACACACCTGCAGTGTCCTTTCCACACTCAGAGGGCCTTTCTGGATCCAGTTCCAG GCCACCCATGGGCCTTGGCCCTGCTGCCTACCCATGGCTGGCAGACTCCTGGCCCCACCCATCTCGAAGT CCCTCAGCTCAGGAACCCCGGGGGAGCTGCTGCCCCAGCAATCCTGATCCAGATGATAGATACTACAATG AGGCAGGAATCTCCCTGTACTTGGCTCAGACTGCACGGGGTGCTAATGCCCCTGGCGAGGGTCCTGTCTA CAGCACCATTGACCCAGTAGGGGAAGAGCTACAGACCTTCCACGGAGGGTTCCCTCAACATTCTTCTGGG GACCCAAGCACCTGGAGCCAGTATGCTCCCCCCGAATGGAGCGAGGGGGACAGTGGAGCCAGGGGAGGCA AAGGGAAGCTTCTAGGCAAACCTGTGCAGATGCCCTCTTTGAGCTGGCCAGAAGCCCTGCCGCCACCTCC CCCTTCCTGTGAATTGAGCTGCCCAGAGGGGCCTGAGGAGGAGCTGAAGGGCAGTTCAGATCTGGAAGAG TGGTGCCCACCAATGCCTGAGAAAAAACACTTGATTGACTCACGTTCCAGTGGTGCGTGCATGGTGGCTC CAGCCCGAGGGGAAACTCCCTCCCCTACCTCTTCTTACGGACAGCAGTCCACAGCCACTCTTACCCCCTC ACCTCCTGACCCTCCCCAGCCCCCTAGCGACATCCCACATCTCCATCAGATGCCCAGGAGGGTGCCCCTT GGACCAAGTTCTCCTCTCAGCGTATCCCAGCCTGCTCTGAGTAGCCATGATGGAAGGCCTGTTGGCCTGG GTGCTGGCCCTATAGTCTCTTATCACCCTAGCCCCAGTCCTGTCCCTAGTACAGCCAGCAGTGCACCAGG GAGAACTCGCCAGGTAACTGGAGAGATGACTCCCCCACTCCATGGACACCGTGCCCGAATCCGGAAGAAA ACCAAGGCTCTTCCACATAGAAGGGAACACAGTCCTGGGGACTTGCCCCCACCACCCTTGCCACCACCAG AGGAAGAGACGAGCTGGCCCTTGGGTCTGAGAGCAGCAGGCAGCATGTCTTCCCTAGAGCGAGAGCACAG TGGGGAGAGGAGAGTGGCCCAGGCAGTGCCTCTAGGGGCCCAACCTCCAGGGGCCCAACGTGGCCCCCAC CCTGATGGTAAGGAGAGCCAGGGCAATGGGAGGGGCCTGGAGGCTTGCAGAAGTCCCCAAAGTCCCCAGA TTCAGTTGGATTCACGTGTCTGGTCAACATTGATATTGTCTCTTATCTCTTTCACTTCCTCTAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108135
Insert Size	3918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108135.1, NP_001101605.1
RefSeq Size	5682 bp
RefSeq ORF	3918 bp
Locus ID	315564

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