Trim50 (NM_181080) Rat Untagged Clone
CAT＃: RN201887
Trim50 (untagged ORF) - Rat tripartite motif-containing 50 (Trim50), (10 ug)
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC93065 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201887 representing NM_181080
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGGCAGCTGACTGTGCCGGAACTCCAGGACCAGCTGCAGTGTCCCATTTGCCTGGAGGTCTTCA AAGAGCCTCTGATGCTTCAGTGTGGCCACTCCTACTGCAAGAACTGCCTGGATAGCTTGTCTGAACACCT GGATTCCGAGCTGCGCTGTCCTGTGTGTCGTCAGTCAGTTGACTGCAGCAGCTCCCCGCCTAATGTCTCC CTGGCCCGGGTGATCGATGCTCTGAGGCTCCCGGGGGACACAGAGCCCACCGTCTGCGTGCACCACCGAA ACCCTCTCAGCCTCTTTTGTGAGAAAGACCAGGAATTCATCTGTGGTCTCTGTGGCCTGCTGGGCTCCCA CCAACACCACAGAGTCACACCAGTCTCCACGGTCTACAGCCGCATGAAGGAAGAGCTTGCAGGCCGCCTG TCTGAGCTGAAGGAGCAGCACAGGGACGTGGAGGAGCACATTGGCAAACTGGTCAACAACAGGACCCGGA TTATTAATGAGTCTGACGTCTTCAGCTGGGTGATCCGAAGAGAGTTCCAGGAGTTGCACCACCTGGTGGA TGAAGAGAAAGCCCGCTGCCTGGAGGGGGTGGAGAGCCACACGCGTGGCCTGGTGGCCTCCCTGGACATG CAGCTGGAGCAGGCTCAGGGTACTCAAGAGCGACTGGCCCAAGCCGAGCGGGTGCTGGAGCAGTTTGGCA ATGAGAGTCACCATGAGTTCATCCGGTTTCACTCCATAACCTCCAGGGGAGAGGTCCAGCAGGCTCGGCC TTTGGAAGGCGTCTTCAGCCCCATCTCCTTCAAGCCCGCTCTCCACCAAGCTGACATCAAACTGACCGTG TGGAAGAGGCTATTCCGGAAAGTTCTGCCAGCTCCAGAATCTCTCAAGCTGGACCCGGCTACTGCCCACC CTCTCCTGGAGCTCTCGAAGGGCAACACAGTGGTGCACTGTGGCCTCCTGGCCCAGAGACGCGCCAGCCA ACCTGAGCGCTTTGACTACAGCACCTGTGTCCTGGCTAGCAAGGGCTTCTCCTGGGGTCGCCACTACTGG GAAGTGGTGGTGGGGAGCAAGAGTGACTGGCGCTTGGGGGTCATCAAGGGCACCGCCAGCCGCAAAGGCA AGCTGAGCAAGTCCCCAGAACATGGGGTATGGCTAATAGGCCTGAAAGAGGGTCGGCTGTATGAGGCTTT TGGCTGCCCACGGCTGCCACTGCCCGTGGCTGGCCACCCTCATCGCATTGGCGTCTACCTACACTATGAG CAGGGTGAGCTCACGTTCTTTGATGCTGACCGTCCTGATGATCTTCGGGCACTCTACACTTTCCAGGCTG ACTTCCAGGGCAAGCTGTACCCTATCCTGGACACATGCTGGCATGAGAGGGGCAGCAACTCACTGCCCAT GGTACTGCCCCCACCCAGTGCGCCTGGCCACCTCACACGTGCACAGGTATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_181080 |
| Insert Size | 1452 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181080.1, NP_851594.1 |
| RefSeq Size | 1452 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 288596 |
| UniProt ID | Q810I1 |
| Gene Summary | E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201887 | Trim50 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 50 (Trim50), (10 ug) |
CNY 6270.00 |
|
| RR201887L3 | Lenti ORF clone of Trim50 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 50 (Trim50), (10 ug) |
CNY 8080.00 |
|
| RR201887L4 | Lenti ORF clone of Trim50 (mGFP-tagged ORF) - Rat tripartite motif-containing 50 (Trim50), (10 ug) |
CNY 8930.00 |
