Nlrp1a (NM_001145755) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nalp1; Nlrp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201861 representing NM_001145755 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAATCTCAGTCCAAGCAGGAAAGTAACACAAGGGTGGCTCAGCATGGAAGCCAACAGGATGTGG ACCCCACCTTCCAAACAAAGAGAGCATTGGAGAAGGAGCGGTCAAAACCCAGACCCCGGCCGCTTCCTCG GGTGCAGCTCCAATCACTTCCTGGATGGTCCAGCACATCCAATGATGTGCCCCTTAGCCAGCTCATCCGT GAAATGGATCATGAGTCAAGGAGATGCATTCATCGATCAAAGAAGAAACTTGACAGGTCTGAACATATAA GCCAAGGGACCATTCCAGAAATATATGAGAAGCGAAAAGAGACTATTTCCCACACACAGTCCATGGAACA AAAGTATTTGTTCCAAAATTTCACAAAGCTACTACTCCTACAAAAATGTTGCCCAGGAGGCTCAGAGAAA TTGGTCAGGGAAAGCTGGCATCCATGTGTACCAGAAGAAGGAGGACATATGATTGAGATCCAAGACTTAT TTGACCCAAACTTAGATACTGAGAAAAAGCCTCAATTAGTCATAATAGAGGGGGCTGCTGGGATTGGGAA GTCAACACTGGCCAGGCAGGTGAAGAGAGCCTGGGACGAAGGCCAGCTCTACAGGGATCGCTTCCAGCAT GTCTTCTTCTTCAGCTGCAGAGAGCTGGCCCAGTGCAAGCAGCTGAGTCTGGCTGAGCTCATAGCCCAAG GCCAGGAAGTGCCCACAGCTCCCACCAGACAGATCCTGTCTCGCCCTGAGAAGCTGCTCTTCATCCTGGA TGGCATAGATGAGCCAGCATGGGTCTTGGAGGACCAGAATCCTGAGCTGTGTGTGCACTGGAGTCAAGCA CAACCTGTGCACACACTGCTGGGCAGTTTACTAGGAAAATCCATCCTTCCTGAGGCTTCCTTAATGCTCA CAGCTCGGACCACAGCTCTACAGAAGCTTGTTCCTTCCTTGGGGCAGCCACATCGAGTGGAAGTCCTGGG GTTCTCTGAGTTTGAACGGAAGGACTATTTCTACAAATACTTTGCAAAAGAAAGAAATACAATTATAGAT TTTAATTTAATTGGATCTATCCCAGTGCTCCTGACCCTATGTGAGGTCCCCTGGGTGTGCTGGTTGCTGT GCACTTGCCTGGAAAAGCAGATGCAGCAGGGTGAAGTACTCTCACTGACCTCACAGACCACCACAGCCCT CTGCCTGAAATACCTTTCCCTGACAATCCCAGGTCAGCACCTGAGCACCCAGCTCAGGACCCTCTGCTCA CTGGCTGCTGAGGGGATCTGCCAAAGAAGGACCCTGTTCAGTAAAAGTGACCTCTGTAAGCAAGGGTTAG CAGAGGATGCCATTGCCACTTTCCTGAAGATCGGTGTCCTTCAAAGGCAGCCCAGCTCTCTGAGCTACAG CTTTGCCCACTTGTGTCTCCAGGAGTTCTTTGCAGCGATGTCCTACATCTTGGAGGATAGTGAGGAAGCA CATGGGGATATGGGAAACGACAGAACTGTGGAAACACTGGTAGAACGGTATGGAAGACAAAACCTGTTTG AGGCACCCACTGTGCGCTTTCTACTTGGTCTTTTAAATACACGGGAAATGAGAGAAATGGAAAATATCTT TGCCTGCAAGTTTCCTTGGGAGACAAAGTTGAAGTTGCTACAGAGCATCATAGGGGAGCCGTTCTGTCAA CCATGTCATCTGGGTTTGTTCCACTGTCTCTATGAAAACCAGGAAGAGGAGCTCCTGACAGAGACGATGC TTTGTTTTCCCTTAACAGCGTCAGGTCCAAATCACATGGAAGCCACGGTGTTCCAGACAAACGTGAAGCG CCTGGTGATACAGACAGACATGGAGCTCATGGTGGTCACTTTCTGCATTACGTTCTCTCATGTGAGGAGT CTTCGGCTGAAGGGGAAAGGACAGCAGGAATATAAGCTAACAGCACCTGCGATGGTTCTGTACAGGTGGA CACCAATCTCTGAGGCCAGTTGGAAGGTTTTATTCTCCAATCTTAAATGCACCAGAAACCTGGAGGAGCT GGACCTAAGTGGAAATCCACTGAGCTACTCTGCAGTACGCAGTCTCTGTACAGCACTGAGACAGCCTGGC TGCCGACTAAAGACCTTGTGGCTTGTGGACTGTGGCCTCACATCCAGATGCTGCTCATTCCTGGCCTCAA TGCTCAGTGCCCACTCAAGGTTGGCTGAACTAGACCTGCGACTGAATGACCTTGGTGACAACGGTGTGAG GCAGCTGTGTGAGGGGCTCAGGAATCCTGCCTGCAACCTCAGCATCCTGCGGCTGGACCAGGCCTCTCTC AGTGAGCAGGTGATTACAGAGCTCAGGGCCCTGGAGACAAAGAATCCGAAGCTGTTCATCTCCAGCACAT GGATGTCACACATGACGATGCCCACTGAAAACACAGATGGAGAAGAGAGCCTGACCTCATCCAAGCAGCA ACAACAACAATCGGGAGACAAACACATGGAACCTCTGGGGACTGATGATGACTTCTGGGGCCCTTCAGGA CCTGTGTCTACTGAGGTGGTTGACAGAGAGAGGAACCTGTACCGAGTGCGATTGCCCATGGCTGGTTCCT ACCACTGTCCCAGCACAGGACTCCACTTTGTGGTGACAAGGGCAGTGACAATCGAGATTGGATTCTGTGC CTGGAGCCAATTCCTGCATGAGACTCCCTTGCAACACAGTCACATGGTGGCTGGACCTCTGTTTGACATC AAGGCTGAGCACGGAGCTGTGACTGCAGTGTGCCTCCCTCATTTTGTGTCCCTCCAAGAGGGAAAGGTGG ACAGCTCCTTGTTCCATGTTGCCCACTTCCAAGACCATGGGATGGTCCTAGAAACGCCAGCAAGGGTGGA ACCACATTTCGCAGTACTGGAAAACCCAAGCTTCTCCCCTATGGGAGTTTTACTGAGAATGATTCCTGCT GTGGGACACTTCATCCCCATCACTTCCATCACATTGATCTACTATCGCCTGTATCTCGAGGACATCACCT TTCACCTTTACCTGGTCCCCAATGACTGCACTATTCGGAAGGCCATTGATGAGGAAGAACTGAAGTTTCA GTTTGTGCGCATAAACAAGCCACCCCCAGTAGATGCTCTTTATGTTGGTTCCCGCTATATCGTGTCCAGC TCCAAAGAGGTGGAAATTCTCCCAAAGGAACTGGAGCTGTGCTACCGAAGCCCTCGGGAATCCCAGCTCT TCTCTGAGATATATGTTGGAAACATTGGTTCAGGGATTAATCTGCAACTAACAGACAAGAAGTATATGAA TCTCATATGGGAGGCCTTGCTGAAACCAGGGGACCTCAGACCTGCACTGCCCAGGATGGCTTCAGCCCCC AAAGATGCTCCTGCCCTGCTACACTTCGTGGACCAGCATCGGGAGCAGCTGGTGGCCCGAGTGACATCAG TGGACCCTCTCTTGGACAAGCTGCATGGTCTGGTGCTGAGTGAAGAAGATTATGAGACAGTGAGGGCTGA GGCCACAAACCAAGACAAGATGAGGAAGCTCTTCAGGGGCAGCCGGTCCTGGAGCTGGGATTGCAAAGAC CATTTCTACCAAGCTCTGAAGGAGACCCATCCTCACCTGATCATGGACCTCCTTGAGAAGTCGGGAGGGG TCTCTGTTAGACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001145755
Insert Size	3657 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001145755.2, NP_001139227.2
RefSeq Size	7024 bp
RefSeq ORF	3657 bp
Locus ID	360557
UniProt ID	D9I2F9
Gene Summary	Acts as the sensor component of the Nlrp1a inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis (By similarity). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (By similarity). Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as B.anthracis lethal toxin (LT) or Val-boroPro inhibitor, and mediates the formation of the inflammasome polymeric complex (PubMed:20502689, PubMed:31383852). In response to pathogen-associated signals, the N-terminal part of Nlrp1a is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (NACHT, LRR and PYD domains-containing protein 1a, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.