Pde4d (NM_017032) Rat Untagged Clone
CAT＃: RN200497
Pde4d (untagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug)
CNY 8650.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | DPDE3; PDE3/IVd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200497 representing NM_017032
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCATGTGAATACCTTCCCTTTCAGAAGGCATTCCTGGATATGTTTCGATGTGGACAATGGCACAT CAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTAATTCTCCAAGCAAACTTTGT GCACAGTCAACGCCGGGAGTCCTTCCTGTATCGATCAGACAGCGATTATGACCTCTCTCCAAAGTCTATG TCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCT TGGCCAGCCTTCGAACTGTAAGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGCGCACCCAGCAA AAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAGGCCTACCAGAAACTG GCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGCAGACCAGGCACTCCG TCAGTGAGATGGCCTCCAACAAGTTCAAGAGGATGCTTAATCGGGAGCTCACCCACCTCTCTGAAATGAG TCGGTCTGGCAACCAGGTGTCGGAGTACATATCAAACACGTTCTTAGATAAGCAACATGAAGTAGAAATT CCCTCTCCGACTCAGAAGGAAAAAGAGAAGAAGAAAAGGCCGATGTCACAGATCAGTGGGGTCAAGAAGT TGATGCACAGCTCCAGCCTGACCAATTCCTGCATTCCAAGATTTGGGGTTAAAACAGAGCAGGAAGATGT CCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATAGCGGAGCTGTCTGGC AACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAAAAACGTTTAAAATCC CAGTGGACACTTTGATTACGTATCTTATGACTCTAGAAGACCATTACCATGCTGACGTGGCCTATCACAA CAACATCCATGCTGCAGATGTCGTCCAGTCAACTCATGTGCTGCTCTCTACACCCGCTTTGGAGGCTGTT TTCACTGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGGATCATCCTGGTGTGT CAAATCAATTTCTGATCAATACAAACTCGGAACTTGCCTTGATGTACAACGACTCCTCCGTCTTAGAGAA TCATCATTTGGCTGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTCCAGAATCTGACCAAA AAGCAAAGACAATCTTTAAGGAAAATGGCCATTGACATTGTACTAGCGACAGACATGTCAAAGCACATGA ATCTGCTGGCTGATCTGAAAACAATGGTTGAAACGAAGAAGGTGACGAGCTCTGGCGTCCTCCTCCTTGA TAACTATTCTGACAGGATCCAGGTCCTCCAGAATATGGTGCACTGTGCAGACCTGAGCAACCCCACAAAG CCACTCCAGCTCTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGTCAGGGGGACCGGGAGC GTGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGAAAAATCACAGGTGGG CTTCATAGACTACATTGTTCATCCACTCTGGGAGACATGGGCAGACCTCGTACATCCCGACGCCCAGGAC ATTTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGAGCCCCTCCCCTGCAC CTGATGACCAAGAGGACGGCCGTCAGGGACAGACTGAAAAATTCCAGTTCGAACTAACCTTAGAGGAAGA TGGCGAGTCAGACACTGAAAAGGACAGTGGAAGTCAAGTGGAGGAAGACACTAGCTGCAGTGACTCTAAG ACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTGAAGAGGAGGCTGTAG CAGAAGAGGAAAGCCAGCCCCAAACTGGCGTCGCAGACGATTGTTGTCCTGACACGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017032 |
| Insert Size | 2019 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017032.1, NP_058728.1 |
| RefSeq Size | 2077 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 24627 |
| UniProt ID | P14270 |
| Gene Summary | may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006] Transcript Variant: This variant (5) has an alternate 5' coding exon, as compared to variant 1. The resulting isoform (5) has a shorter N-terminus, as compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200497 | Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug) |
CNY 8650.00 |
|
| RR200497L3 | Lenti ORF clone of Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug) |
CNY 10450.00 |
|
| RR200497L4 | Lenti ORF clone of Pde4d (mGFP-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug) |
CNY 11590.00 |
