Itsn1 (NM_001136096) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	EHSH1; Itsn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200380 representing NM_001136096 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTTCCGACACCTTTTGGTGGGAGCCTGGACATCTGGGCCATAACCGTGGAGGAAAGAGCCA AGCATGACCAGCAGTTCCAGAGTTTGAAGCCGATATCTGGATTCATCACTGGTGATCAAGCAAGGAACTT CTTTTTCCAATCTGGGTTACCTCAGCCCGTCTTAGCACAAATATGGGCGCTGGCTGACATGAATAAGGAC GGAAGGATGGATCAGGTGGAGTTTTCCATAGCCATGAAGCTCATCAAACTGAAGCTGCAAGGATATCAGC TCCCCCCCGCACTTCCCCCTGTCATGAAGCAGCAGCCAGCGGCCATCTCTAGTGCACCAGCGTTTGGTAT AGGAGGGATGGCTGGAATGCCACCACTGACAGCTGTTGCTCCCGTGCCAATGGGCTCCATCCCAGTTGTT GGAATGTCTCCGCCCTTAGTATCTTCTGTCCCTCAAGCAGCAGTGCCTCCCCTGGCTAACGGGGCCCCTC CTGTCATACAGCCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTCAGCAG ATCTGGTCCAGGGTCACAATTAAACACTAAACTACAGAAGGCACAATCATTTGATGTAGCCAGCGCCCCT GCAGCGGCAGAATGGGCTGTGCCTCAGTCGTCAAGACTGAAATACAGGCAGTTATTCAACAGTCACGACA AGACCATGAGTGGACACTTAACAGGTCCCCAGGCAAGAACTATTCTCATGCAATCAAGTTTACCCCAGGC TCAGCTGGCTTCAATCTGGAATCTTTCCGACATTGATCAAGATGGAAAGCTCACCGCAGAAGAATTCATC CTAGCGATGCACCTGATTGATGTTGCTATGTCTGGTCAGCCACTGCCGCCTGTCCTGCCTCCAGAATACA TCCCTCCTTCCTTTAGAAGAGTTCGCTCTGGCAGTGGGATGTCCGTCATAAGCTCTTCGTCTGCAGACCA GCGGCTGCCCGAGGAGCCATCGTCAGAGGATGAGCAGCAGGTGGAAAAGAAGCTGCCTGTGACATTTGAA GATAAGAAGCGGGAGAACTTCGAGCGAGGCAACCTGGAGCTGGAGAAGCGCAGGCAGGCCCTCCTGGAGC AGCAGCGCAAGGAGCAGGAGCGGCTGGCTCAGCTGGAGCGCGCCGAGCAAGAGAGGAAAGAGCGGGAGCG CCAGGAACAGGAGCGCAAGAGACAGCTAGAGCTGGAGAAGCAGCTAGAAAAGCAGCGGGAGCTGGAGAGG CAGAGAGAGGAGGAGAGAAGGAAGGAGATCGAGAGGCGGGAGGCAGCAAAACGGGAGCTCGAACGGCAGC GACAGCTTGAATGGGAACGGAACCGGAGACAGGAACTCCTGACTCAGAGGAACAAGGACCAGGAGGGCAT CGTGGTTCTGAAGGCGAGGAGGAAGACTCTGGAGTTTGAATTAGAAGCTCTGAATGACAAAAAGCATCAG CTGGAAGGAAAACTTCAGGATATCAGGTGTCGACTGGCAACCCAGAGGCAAGAAATTGAGAGCACAAACA AGTCTAGAGAGCTGAGAATTGCGGAAATCACCCACTTACAGCAACAGTTGCAGGAATCTCAGCAGATGCT TGGAAGACTTATTCCAGAGAAGCAGATACTCAGTGACCAGTTAAAACAAGTCCAGCAGAACAGCTTGCAT AGAGATTCACTTCTTACCCTCAAAAGAGCCTTGGAAGCAAAGGAACTGGCCCGGCAGCAGCTTCGAGAGC AGCTGGACGAGGTGGAGAAAGAGACCAGGTCAAAGCTGCAGGAGATTGATGTTTTCAACAACCAGCTGAA GGAACTGAGAGAGATACACAGTAAACAGCAGCTCCAGAAGCAGAGGTCCATCGAGGCGGAGAGGCTAAAG CAGAAAGAGCAGGAGAGGAAGAGCCTGGAGTTGGAGAAGCAAAAGGAAGAAGGTCAGAGACGAGTTCAGG AAAGGGACAAGCAATGGCAGGAGCATGTGCAGCAGGAGGAGCAGCAGCGCCCTCGGAAACCCCACGAGGA GGACAAACTGAAAAGGGAAGACAGTGTCAAGAAGAAGGAGGCGGAAGAGAGAGCCAAGCCGGAAGTGCAA GACAAGCAGAGTCGGCTTTTCCATCCACATCAAGAGCCAGCTAAGCCGGCGCAGGCACCCTGGCCCACGA CAGAGAAAGGTCCGCTTACAATCTCTGCACAGGAGAGTGCCAAAGTGGTGTATTACCGAGCGCTGTACCC CTTTGAGTCCAGAAGTCATGACGAGATCACCATCCAGCCAGGAGATATAGTCATGGTGGATGAAAGCCAG ACCGGAGAGCCAGGATGGCTTGGAGGAGAACTGAAGGGGAAGACAGGATGGTTCCCTGCAAACTATGCAG AGAAGATTCCAGAAAATGAGATTCCCACTCCAGCCAAACCAGTGACCGATCTGACATCTGCCCCTGCCCC CAAACTGGCTCTGCGTGAGACCCCTGCTCCTTTGCCAGTGACCTCTTCTGAGCCCTCCACAACCCCCAAC AACTGGGCAGACTTCAGTTCCACGTGGCCCAGCAGCACAAATGAGAAACCAGAAACGGACAACTGGGACA CGTGGGCGGCTCAGCCTTCTCTGACTGTACCCAGTGCTGGCCAGTTAAGGCAGAGGTCGGCCTTCACACC AGCCACAGCCACTGGCTCCTCCCCTTCTCCCGTCCTGGGCCAGGGTGAAAAGGTGGAAGGGCTCCAAGCA CAAGCCCTGTATCCCTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAGTGATGTCATCACCG TTCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGGTTCCCCAAGTCTTA CGTGAAACTCATTTCAGGGCCCGTAAGGAAATCCACAAGCATCGATACTGGCCCTACGGAAGCTCCCTCT AGTCTAAAGAGAGTGGCTTCCCCAGCCGCCAAGCCAGCCATTCCTGGAGAAGAATTTGTTGCTATGTACA CTTACGAGAGTTCTGAGCATGGAGATTTAACCTTTCAGCAAGGGGATGTGATTGTGGTTACCAAGAAGGA TGGTGACTGGTGGACGGGCACAGTGGGCGAGACGTCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAA GACTCAGAGGGCTCTGGAACTGCGGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATCGCCCAGGTCA TTGCTTCCTACACTGCTACGGGTCCTGAACAGCTCACCCTGGCTCCTGGTCAGCTGATTTTGATCCGAAA AAAGAACCCCGGTGGATGGTGGGAAGGAGAACTACAAGCTCGAGGGAAAAAGCGCCAGATAGGGTGGTTC CCAGCAAATTATGTCAAACTTCTAAGCCCTGGAACAAGCAAAATCACCCCAACTGAGCTACCCAAGACCG CAGTGCAGCCAGCAGTGTGCCAGGTGATCGGGATGTACGATTACACCGCGCAGAATGACGATGAGCTAGC CTTCAGCAAAGGCCAGATCATCAACGTCCTCAGCAAGGAGGACCCGGACTGGTGGAAAGGAGAAGTCAGT GGGCAGGTTGGGCTCTTCCCGTCCAATTATGTGAAGCTGACCACAGACATGGACCCCAGCCAGCAATGGT GCTCAGACTTACATCTCTTAGATATGTTGACCCCGACTGAGAGGAAGAGGCAAGGCTACATCCATGAACT CATTGTCACAGAGGAGAACTATGTGAACGACCTGCAGCTGGTCACAGAGATCTTTCAGAAACCCCTGACG GAGTCTGAGCTGCTGACAGAAAAAGAGGTTGCTATGATTTTTGTTAACTGGAAGGAGCTGATTATGTGTA ATATCAAACTGCTGAAGGCGCTGAGAGTCCGCAAGAAGATGTCCGGAGAGAAGATGCCGGTGAAGATGAT CGGTGACATCCTGAGCGCCCAGCTGCCCCACATGCAGCCTTACATCCGCTTCTGCAGCTGCCAGCTCAAT GGGGCCGCCCTCATCCAGCAGAAGACAGATGAGGCTCCAGACTTCAAGGAGTTCGTCAAAAGACTGGCAA TGGACCCTCGGTGCAAAGGCATGCCACTGTCCAGCTTCATACTGAAACCTATGCAGCGTGTCACAAGATA CCCACTGATCATTAAAAACATCCTGGAAAACACTCCTGAAAACCACCCCGACCACAGCCACCTGAAGCAC GCCCTGGAGAAAGCAGAAGAGCTGTGCTCCCAAGTGAACGAGGGAGTTCGAGAGAAGGAGAACTCTGACC GGCTGGAGTGGATCCAGGCGCATGTGCAGTGCGAAGGCCTTTCTGAGCAACTGGTGTTCAATTCGGTGAC CAACTGCTTGGGACCACGCAAGTTTCTGCACAGCGGGAAGCTCTACAAGGCCAAGAGCAACAAAGAGCTG TATGGCTTCCTCTTCAATGACTTCCTCCTGCTGACCCAAATCACAAAGCCCTTAGGCTCTTCCAGCACTG ACAAAGTCTTCAGCCCCAAATCTAACCTGCAGTATAAAATGTACAAAACGCCCATTTTCTTAAATGAGGT TCTAGTAAAATTGCCCACGGACCCTTCTGGAGATGAACCTATCTTTCACATTTCGCACATTGACCGAGTC TACACGCTCCGAGCAGAAAGTATAAATGAGAGGACTGCCTGGGTGCAGAAAATCAAGGCCGCATCTGAGC TCTACATAGAGACGGAGAAAAAGAAGCGAGAGAAGGCGTATCTGGTCCGTTCCCAGAGAGCGACCGGTAT TGGAAGATTGATGGTGAACGTAGTAGAAGGCATTGAGCTGAAGCCCTGTCGGTCACATGGAAAGAGCAAC CCGTACTGTGAGGTGACCATGGGCTCTCAGTGCCACATCACCAAGACAATCCAGGACACGCTGAACCCTA AGTGGAATTCTAACTGCCAGTTCTTCATCAGAGACCTGGAACAGGAGGTTCTCTGCATCACTGTGTTTGA GAGGGACCAATTCTCGCCAGACGATTTTTTGGGTCGGACAGAGATCCGAGTGGCCGACATCAAGAAAGAC CAGGGCTCCAAGGGGCCAGTTACAAAGTGTCTCCTGCTGCATGAGGTGCCCACAGGAGAGATTGTGGTTC GCCTTGACCTGCAGTTGTTTGATGAGCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001136096
Insert Size	5142 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001136096.1, NP_001129568.1
RefSeq Size	5203 bp
RefSeq ORF	5142 bp
Locus ID	29491
UniProt ID	Q9WVE9
Gene Summary	binds to dynamin and SNAP-25; may be involved in exocytosis [RGD, Feb 2006] Transcript Variant: This variant (1) encodes the longer isoform (a).

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