Unc13b (NM_001042579) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Munc13-2; Unc13h2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200305 representing NM_001042579 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGCTCTGCGTGCGTGTTAAGAGAGCCAAATTCCAGGGTTCACCAGATAAGTTTAACACATACG TGACCCTGAAAGTCCAGAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCA AGATTTCATGTTTGAGATCAGCCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAAGGGCTGATA TGGGACACAATGGTGGGGACCGTGTGGATCGCGCTGAAGACTATCCGCCAGTCGGATGAGGAAGGGCCTG GGGAGTGGTCTACACTGGAGGCTGAGACATTAATGAAAAATGATGAGATCTGTGGAACTAAAAACCCAAC TCCTCATAAAATCTTGCTTGGTACACGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATAT TGGACCTACAAATTGGAACAAATAAATGCCTTGGCAGATGATAATGAGTATTCCAGTCAAGAAGAAAGCC AGAGGAAGCTGTTGCCCACTGCTGCCGCCCAGTGTCGTCACTGGACCTATTTGGGCTGGGGAGAGCATCA GACCTTTGAAGACCCTGATAGTGCCGTTGATGACCGGGACAGTGACTATCGAAGTGAGACCAGCAATAGT GCCCCACCTCCTTATCACACGACTACCCAGCCCAACGCGTCTGCACACCAGTTCCCTATGCCAGTGCCAC TGCCACAGCAGCTATTTCTTCAGGGAAGTTCCCGAGACTCCTGCAATGACTCTATGCAGAGTTATGACCT TGATTACCCAGAGCGTCGGGCCCTCAGCCCCACTAGCAGTAGTAGGTATGGCTCCTCCTGTAATGTGAGT CGAGGAAGCTCTCTGCTGAGTGAACTGGACCAGTATCATGAGCAAGACGATGATGGTCGGGAGAGGGACT CCATTCATTCTAGCCATAGCTATGGCAGCCTCTCCAGAGATGGCCAGGCTGGCTTGGGAGAACAAGAGAA AGCCTTGGAAGTGGCCTGTGAATCACAGAAGGAGAAAACAGGTGAATCCAAGGAGAGAGATGATGCCACA GTCTGCCCTCCGTCAGACCTGATGCTGCACAAGGACCTCACCCTAGGACCCCAGGAGAGTTTTCCCGAGG AGAAAGCATCGTCGCCCTTTACCCAGGCCAGAGCACACTGGTTCCGAGCGGTTACCAAGGTCCGACTCCA GCTGCAGGAGATTTCAGATGATGGTGACCCCTCCTTGCCTCAGTGGCTCCCAGAAGGGCCAGCGGGAGGG CTCTATGGCATCGACAGCATGCCAGATCTACGAAGAAAGAAGCCACTGCCACTTGTCAGTGATCTGGCTA TGTCACTGGTCCAGTCACGGAAAGCAGGGATTACTTCCGCTATGGCTACACGTACCTCTCTCAAGGATGA AGATCTGAAATCCCATGTGTATAAGAAAACTCTGCAGGCCTTAATCTACCCCATCTCGTGCACCACGCCC CACAACTTTGAGGTCTGGTCTGCCACGACACCCACCTACTGCTATGAGTGTGAAGGCTTGCTCTGGGGCC TTGCCCGGCAAGGCATGCGCTGCAGCGAGTGTGGCGTCAAGTGCCATGAGAAGTGCCAAGACCTGCTCAA TGCCGATTGCCTACAGCGGGCCGCTGAAAAGAGCTCTAAGCACGGAGCTGAGGACCGGACTCAGAACATT ATCATGGCCATGAAGGACCGTATGAAGATCCGAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGG ATGTGTTCACAGTGAGCAAAGTTGCCCACGTGCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGG CACCTCCAAGTGGTCGGCCAAAATTACCATCACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAAG ACAGGATCCAGTGACCCTTATGTGACTGTGCAAGTTGGGAAAACCAAGAAGCGCACTAAGACCATTTTTG GAAATTTGAATCCTGTTTGGGAGGAGAAGTTCCATTTTGAGTGCCACAACTCTTCCGATCGAATTAAGGT ACGTGTGTGGGATGAGGATGATGACATCAAATCCAGAGTAAAGCAGCGGCTGAAGCGAGAGTCGGATGAT TTCCTTGGCCAAACTATCATTGAAGTTCGAACGCTGAGTGGAGAGATGGATGTCTGGTATAATCTGGAGA AGAGAACAGACAAGTCAGCTGTCTCAGGGGCTATCCGACTGCAAATCAATGTGGAGATCAAGGGGGAGGA GAAGGTAGCCCCATACCATGTGCAATACACATGTCTCCATGAGAATCTTTTCCATTACCTCACAGACATT CAGGGCAGTGGAGGAGTCTGGATCCCAGACGCTCGGGGGGACGATGCATGGAAGGTGTACTTTGACGAGA CAGCCCAAGAAATTGTGGATGAATTTGCCATGCGCTATGGCATCGAGTCCATATATCAGGCCATGACGCA CTTTGCGTGTTTGTCATCCAAGTACATGTGTCCTGGCGTACCAGCCGTGATGAGTACCTTACTGGCCAAC ATCAATGCGTATTATGCTCACACAACCGCCTCCACTAACGTCTCTGCATCTGACCGCTTTGCCGCCTCCA ACTTTGGTAAAGAAAGATTTGTAAAACTGCTGGACCAGCTACACAACTCACTGAGGATAGACCTGTCTAC ATACAGGAATAACTTTCCTGCTGGGAGCCCTGAGCGGCTCCAGGATTTAAAATCTACAGTGGACTTGCTG ACCAGCATTACTTTCTTCAGGATGAAGGTGCAAGAACTTCAGAGCCCCCCAAGAGCCAGCCAGGTGGTAA AGGACTGTGTGAAGGCCTGCTTGAACTCTACATATGAATACATCTTCAACAACTGCCATGACCTCTACAG TCACCAATACCAGCTGCAGGAACAACCACTAGAGGAACCAGGGCCCAGCATTCGGAACTTGGATTTCTGG CCCAAACTTATCACACTGATTGTATCAATCATAGAGGAGGATAAGAATTCCTACACACCTGTTCTGAACC AGTTTCCTCAGGAGTTGAATGTAGGAAAAGTCAGTGCAGAAGTGATGTGGCACCTGTTTGCTCAAGACAT GAAATATGCACTGGAGGAACATGAGAAAGACCGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTC AAGGTGAAGTGGCTCCACAATGAATATGTGCGGGAGCTGCCTGCCCTCCAGGGGCAGGTGCCTGAGTACC CAGCGTGGTTTGAGCAGTTCGTGCTACAGTGGCTAGATGAAAATGAAGACGTGTCCCTGGAATTCCTTCG TGGGGGCTTGGAACGAGATAAGAGAGATGGGTTCCAGCAAACATCGGAGCATGCCTTGTTTTCCTGTTCT GTGGTGGACGTCTTTACACAACTCAACCAGAGCTTTGAGATCATCCGGAAATTGGAATGTCCAGACCCCA GCATCCTTGCCCATTATATGAGAAGGTTTGCTAAGACCATCGGCAAAGTGCTGATACAGTATGCAGACAT CTTATCAAAGAACTTCCCAGCTTACTGCACAAAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAG CAACTGAGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAAGAGCTGGACTCTGAAGCTGCAG ACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACAGTTCTGGATGAGCTCAGCATGGTGTTTGGAAACAG CTTCCAGGTGCGGATCGACGAGTGTGTTCGACAAATGGCTGACATCCTGGGCCAAGTACGGGGCACAGGG AATGCATCCCCTAACGCCAGAGCCTCGGTGGCTCAGGATGCAGATAGTGTGCTGCGACCTCTCATGGATT TCCTGGATGGCAACCTCACACTGTTTGCCACTGTGTGTGAGAAGACAGTGCTGAAGCGAGTCCTGAAGGA GCTCTGGCGAGTGGTGATGAACACCATGGAGCGGGTCATCGTCCTGCCCCCACTCATTGACCAGACAGGC ACCCAGCTGATCTTAACTGCTGCCAAGGAGCTGAGCCAGCTTTCCAAACTCAAGGACCACATGGTACGAG AGGAAACACGGAATCTCACTCCAAAGCAGTGTGCTGTCCTCGACTTGGCCTTGGACACCGTCAAGCAATA CTTCCATGCAGGAGGCAATGGTCTGAAGAAAACCTTCCTGGAAAAGAGCCCAGACCTGCAGTCTCTACGT TATGCCCTCTCTCTGTATACACAGACCACAGACACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCC AAGTACATGATGGGAAAGGTATTAGGTTTACTGCTAACGAGGACATTCGGCCAGAAAAGGGGGCTGGTGT GGACGACCCTGTGGGAGAAGTCTCTATTCAGGTGGACTTGTTTACACATCCTGGCACTGGGGAGCATAAG GTCACAGTGAAAGTGGTAGCAGCCAATGATCTCAAGTGGCAGACAGCAGGTATGTTCCGGCCTTTTGTGG AAGTGACCATGGTTGGCCCACACCAAAGCGATAAGAAGAGGAAGTTCACAACCAAGTCTAAAAGTAACAG CTGGACTCCCAAGTACAACGAGACTTTTCATTTCCTCCTGGGAAATGAAGAGGGTCCCGAGGCCTATGAA TTGCAGATATGCGTGAAGGATTACTGCTTTGCCCGGGAGGATCGTGTGATAGGACTGGCAGTGATGCCCC TGAGGGATGTGGCAGCTAAGGGCAGCTGTGCCTGCTGGTGCCCGTTGGGCCGGAAGATCCACATGGATGA GACAGGCATGACCATTCTCCGGATTCTGTCTCAGAGGAGCAATGATGAGGTAGCCCGAGAGTTCGTGAAG CTCAAGTCAGAGTCTCGATCCATAGAGGAAGGGAGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001042579
Insert Size	4869 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001042579.1, NP_001036044.1
RefSeq Size	5119 bp
RefSeq ORF	4869 bp
Locus ID	64830
UniProt ID	Q62769
Gene Summary	brain specific protein that may have a role in neurotransmitter release [RGD, Feb 2006] Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an alternate AUG and result in a shorter isoform (u), compared to isoform b.

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