Rims2 (NM_053945) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nim2; Rim2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200223 representing NM_053945 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTCCACTCGGGCCCCGGGGCCGCCCGGCTCCCACCCCGGCGGCCTCTCAGCCTCCTCCGCAGC CCGAGATGCCGGACCTCAGCCACCTCACGGAAGAGGAGAGGAAAATCATCCAGGCTGTCATGGATCGTCA GAAGAAAGAAGAGGAGAAGGAGCAGTCCGTGCTCAAAAAGCTGCATCAACAATTTGAAATGTATAAGGAG CAAGTCAAGAAAATGGGAGAGGAATCACAACAGCAGCAAGAGCAGAAGGGCGACGCCCCGACCTGCGGCA TCTGCCACAAGACAAAATTTGCAGATGGATGCGGCCATAACTGTTCATATTGCCAAACCAAGTTCTGTGC TCGTTGTGGAGGTCGAGTGTCATTACGCTCAAACAAGGTTATGTGGGTGTGTAATTTGTGCCGAAAACAA CAAGAAATCCTCACTAAGTCGGGCGCGTGGTTTTACAATAGTGGGTCTAATACACCGCAGCAGCCTGATC AAAAGGCTCTTCGAGGGCTTCGCAGTGAGGAAGCCCCTCAGGAGAAGAAGGCAAAACTGCATGAGCAGAC GCAGTTCCAGGGACCCCCCGGTGACTCATCAGTACCTGCAGTTGAGAGAGGTCGAGCTCATGGGCTCACA AGACAGGATTCTATTAAGAATGGCTCAGGAATGAAGCACCAGATCGCCAGTGACATGCCTTCAGACAGAA AAAGAAGTCCGTCAGTGTCCAGGGATCAAAATCGAAGATACGACCAAAGTGAAGAAAGAGAGGAATATTC ACAGTATGTTCCTTCAGATAGCACAATGCCTAGATCTCCATCAGATTATGCTGATAGACGATCTCAACGT GAACCTCAATTTTATGAAGAACCTGATCATTTAAATTATAGGGATTCTAACAGGAGAGGCCATAGACATT CCAAAGAGTATATTGTAGACGACGAAGATGTGGAGAGCAGAGATGAATATGAAAGACAAAGGAGAGAGGA GGAGTACCAGGCACGCTACAGAAGTGATCCAAATTTGGCCCGGTATCCGGTAAAGCCACAACCCTATGAA GAGCAAATGCGGATCCACGCTGAAGTGTCCCGGGCACGACACGAGAGAAGGCACAGTGATGTTTCTTTGG CAAATGCTGAACTAGAAGATTCCAGGATTTCTCTGCTAAGGATGGATAGACCATCAAGGCAAAGATCTGT ATCTGAACGTAGAGCTGCAATGGAAAACCAGCGATCGTATTCAATGGAAAGAACTCGAGAAGCTCAGGGA CAAAGTTCTTATCCACAAAGGACCACAAATCATAGTCCTCCTACCCCTCGCAGGAGCCCTATACCGCTCG ATAGACCAGAGCTGAGGCGTGCCGACTCCCTACGGAAACAACACCACTTAGATCCCAGCTCTGCTGTAAG GAAAACGAAGCGAGAAAAAATGGAAACAATGTTGAGGAATGATTCTTTGAGTTCAGACCAGTCTGAGTCA GTGAGGCCGCCCCCACCAAGGCCTCATAAATCCAAGAAAGGAGGTAAAATGCGCCAGGTTTCACTGAGCA GCTCAGAGGAGGAGTTGGCATCCACGCCTGAGTATACAAGCTGTGATGACGTGGAGATTGAAAGCGAGAG CGTAGGTGAGAAAGGAGACATGGAGTACAGCTGGTTGGAGCATGCGTCTTGGCATAGCAGTGAGGCATCC CCAATGTCTTTGCACCCTGTGACCTGGCAGCCATCCAAAGATGGAGATCGCCTAATTGGTCGTATTTTAT TAAATAAGCGTTTAAAAGATGGGAGTGTACCTCGAGATTCAGGAGCAATGCTGGGCTTAAAGGTTGTAGG AGGAAAGATGACTGAATCAGGTCGACTTTGTGCATTTATTACCAAAGTGAAAAAAGGAAGTTTAGCTGAT ACTGTAGGACATCTTAGACCAGGTGATGAAGTCTTGGAATGGAATGGAAGGCTATTGCAAGGAGCCACAT TTGAGGAAGTTTACAACATTATTCTAGAATCCAAGCCTGAACCACAAGTTGAGCTTGTTGTTTCAAGGCC GATTGGAGATATGCCTAGAATACCTGATAGCACTCATGCACAACTGGAATCCAGTTCTAGCTCATTTGAA TCTCAAAAAATGGATCGTCCGTCTATATCGGTTACCTCTCCCATGAGTCCTGGCATGCTGAGGGATGTCC CACAGTTCTTATCTGGACAGCTTTCAAGCCAAAGCCTTAGTAGAAGAACAACGCCTTTTGTTCCTAGGGT TCAGATAAAACTATGGTTTGACAAGGTTGGTCACCAGTTAATAGTTACAATTTTGGGAGCAAAGGATCTC CCTTCCAGGGAAGATGGGAGGCCAAGGAATCCTTATGTTAAAATTTACTTCCTTCCAGACAGAAGTGATA AAAACAAGAGAAGAACAAAAACAGTCAAGAAAACTTTGGAACCCAAATGGAACCAGACTTTCATTTATTC TCCTGTTCACCGAAGAGAATTCCGGGAACGAATGCTCGAAATCACCCTTTGGGATCAAGCTCGAGTTCGA GAGGAAGAAAGTGAATTCCTAGGAGAGATTTTAATTGAATTGGAAACAGCTCTGTTAGATGATGAACCAC ACTGGTACAAACTTCAGACCCATGATGTCTCCTCATTGCCACTCCCTCACCCTTCTCCATATATGCCACG GAGACAGCTGCATGGAGAGAGCCCAACACGCAGGTTGCAAAGGTCGAAGAGAATAAGTGACAGCGAAGTA TCCGACTATGACTGCGAGGACGGCGTGGGAGTCGTGTCAGATTACCGACACGATGGCCGTGATCTTCAAA GCTCCACATTATCAGTGCCAGAACAAGTCATGTCATCAAACCATTGCTCACCATCAGGCTCTCCTCATCG AGTAGATGTTATAGGAAGGACTAGGTCATGGTCGCCTAGTGTCCCTCCTCCTCAAAGGAATGTGGAACAG GGGCTTCGAGGGACACGTGCTACTGGCCATTACAATACAATTAGCCGGATGGACAGACACCGTGTCATGG ACGACCATTACTCTTCAGAGAGAGACAGTCATTTTCTCACTCTACCTCGCTCTCGACACAGGCAGACCAG TGAGCATCACCACAGGGATGGAAGGGATTGTGAAGCAGCAGATAGACAGCCATATCACAGATCCAGATCA ACAGAACAACGGCCTCTCCTAGAGCGGACCACCACCCGCTCCAGATCCTCTGAACGTGCTGATACAAACC TCATGAGGTCGATGCCTTCATTAATGACTGGAAGATCTGCCCCTCCTTCACCTGCCTTATCGAGGTCTCA CCCTCGCACTGGCTCTGTCCAGACCAGTCCGTCAAGTACTCCCGTAACAGGACGAAGGGGCCGGCAGCTT CCTCAGCTCCCACCAAAGGGAACACTGGAAAGAATGATCACAGAAGACATGGACTCCACAAGGAAGAGAA ACTCAGGTGCTATGGATATAGAGGAGAGAAATCGCCAAATGAAACTTAACAAATACAAACAGGTAGCCGG ATCAGATCCCAGACTGGAACAAGATTACCATTCGAAGTATCGCTCAGGATGGGATCCACATAGAGGGGCA GATACTGTTTCTACTAAATCCTCGGACAGTGATGTAAGTGATGTATCTGCGGTTTCAAGGACTAGTAGTG CTTCTCGTTTCAGCAGCACAAGCTACATGTCCGTCCAATCAGAACGGCCGAGAGGAAACAGGAAAATCAG TGTCTTTACGTCCAAAATGCAAAGCAGACAGATGGGTGTGTCGGGGAAGAGCATGGCCAAAAGCACCAGC ATCAGTGGAGACATGTGCTCACTGGAGAAGAATGATGGCAGCCAGTCGGACACTGCAGTGGGCGCCCTGG GCACCAGTGGCAAGAAGCGGCGATCTAGCATTGGGGCCAAAATGGTAGCTATTGTTGGTCTCTCACGGAA AAGTCGCAGTGCCTCCCAACTCAGCCAAACCGAAGGAGGAGGTAAAAAGCTACGCAGCACTGTCCAGAGA AGCACGGAGACCGGGCTAGCAGTGGAGATGAGGAACTGGATGACAAGACAGGCCAGCCGGGAATCCACGG ATGGCAGCATGAACAGTTACAGCTCAGAAGGAAATCTGATCTTTCCTGGGGTCCGCCTGGCCTCTGACAG CCAGTTCAGCGATTTCCTTGATGGTCTGGGCCCTGCTCAGCTAGTGGGACGCCAGACCCTGGCGACTCCT GCAATGGGTGACATTCAGGTGGGGATGATGGATAAGAAGGGACAGCTGGAGGTAGAGATCATCCGGGCTC GAGGCCTTGTAGTAAAACCAGGTTCCAAGACACTGCCAGCACCATATGTCAAGGTGTACCTGTTAGACAA CGGAGTCTGCATAGCCAAAAAGAAAACCAAGGTGGCGAGAAAGACGCTGGAGCCCCTGTATCAGCAGCTG TTATCTTTCGAAGAGAGCCCCCAGGGGAAGGTGTTACAGATCATTGTCTGGGGAGATTATGGCCGCATGG ATCACAAATCCTTTATGGGAGTGGCCCAGATACTCTTAGATGAACTGGAACTATCCAACATGGTAATTGG ATGGTTCAAACTCTTCCCTCCTTCCTCCCTAGTAGATCCAACCTTGGCACCTCTGACAAGAAGAGCTTCC CAATCGTCTCTGGAAAGTTCTACTGGACCTTCTTACTCTCGTTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053945
Insert Size	4668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053945.2, NP_446397.1
RefSeq Size	5639 bp
RefSeq ORF	4668 bp
Locus ID	116839
UniProt ID	Q9JIS1
Gene Summary	synaptic protein important for normal neurotransmitter release; binds Rab3a [RGD, Feb 2006]

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