Nrap (NM_001113743) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200098 representing NM_001113743 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATGCAGGCCTGCTCTAGGTGTGGCTATGGGGTTTACCCTGCTGAGAAGGTCAGCTGCATAGACC AGACATGGCACAAAGCATGTTTTCACTGCGAGGTCTGCAAGATGATGCTTTCTGTTAATAACTTCGTGAG TCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACGTTCACTAGTGTCTACCGCACA CCTTTAAATCTGACCTTGAAGAATTCTGTGGCAGCCATGGGTGGGACCGACGGCAAAGAAGACGGCGAAC AGTTTAAATCCGTTCTCCATTGGGACATGAAGTCCAAGGCCGGAGCGGGGGCGGCTAGCCGGCTGATGAA CGAGAGAGATTACTGGCCAGGGTATGGAGAAGGGAACACTTGGTGTCCAGGAGCTCTTCCAGACCCTGAG ATTGTAAGGATGGTTGAAGCTCGACAGTCTCTCCGTGAGGGGTACACAGAAGATGGTGAGCAACCGCAAG GCAAAGGGAGCTTCCCAGCCATGATCACCCCTGCCTACCAACGGGCCAAGGCAGCCAACCAGCTGGCCAG CCAGGTGCAATACAAGAGAGGCCACGATGAACGGGTCTCCACGTTCACTCCGGTGGCAGACACTCCCGAG CTGCTACGGGCCAATGCTGGGGGACAGCTTCAAAATGATGTGAGATACACGGAAGACGGAGGGCAGCAGA GAGGGAAAGGCAGTTTCCCTGCTATGATCACACCAGCGTATCAGATAGCCAAAAGAGCCGCCGAGCTGGC CAGCGATGTGAGGTACCATCAACAATATCACAGAGAAATGAAGGGAGTGGCTAGTCCTGTCGGAGCTGAG GGCACGATGACAAAGGACTCTATAGACCGATGTGGCCAGACTTACTCAGAAGAGTGTGAGGAACATAGGG GAAAGGGCAGCTTCCCGGCCATGATCACCCCAGCTTATCAGAACGCCAAGAAGGCCAACGAACTTGCTAG TGACATAAAGTACAGGCAGGACTTCCATAAGATGAAGGGCGCGGCACATTTTCACTCCCTTGCAGCCCAG GACAACTTGGTTCTCCAGCGGGCTCAGAATGTGAGCAAGCTTGTGAGTGAGGTGGAGTACAAGAAGGGTC TGGAGAATAGTAAAGGTCACAGTATCAACTACTGTGAGACACCCCAGTTCAGGAATGTGAGCAAGATCTC AAAATTCACCAGCGATAATAAGTATAAAGAAAACTATCAGACTCACCTGAGAGGCCACTATGAAGGAGTC GGCATGGACAGACGCATGCTCCACGCTCTCAAAGTCGGCAGCCTGGCCAGCAATGTTGCCTACAAGGCTG ACTATAAACACGACGTTGTGGATTACAACTACCCAGCCACTGCCACTCCGTTCTACCAAACCACGATGAA GCTGGCTCCCCTGAAGGATGTCAACTACAGGCAGAACATCGACAGACTGAAGTTCAGCTCGGTGACAAAC ACGCCACAGATTGTTCAAGCTAAAATCAACGCCCAGCAGCTGAGCCATGTGAATTACCGAGCTGATTACG AGAGAAACAAGCTGAACTACACATTGCCCCAGGACGCTCCTCAGATGCTGAAGGCGAAGGCCAACGCGCA ACTGTTCAGCGAGGTGAAGTACAAAGAAGGCTGGCAGAAGACAAAGGGGAAAGGATTTGAGATGAAGCTG GACGCCATGTCTCTGTTGGCCGCCAAAGCCTCGGGGGAGCTTGCTAGCAGCATTAAATACAAAGAAGAGT ATGAGAAAACAAAGGGCAGAGTCCTGGGAACCACCGACTCCAAGCTTCTGCATTCTCTGCAGGTGGCCAA GATGAGCAGCGAGGTGGAATATAAGAAAGGGTTTGAGGAGAGTAAAACCCACTTCCACTTGCCCATGGAC ATGGTGAACCTCAGGCATGCTAAGAAGGCCCAGGCCCTCGCCAGCGACCTCGACTACAGAAAGAAACTGC ATGACTACACAGTGCTGCCTGAAGATCTGAAAACTCAGTGGGCCAAGAAAGCCCACGGGCTCCAGAGTGA GCTGCAGTACAAAGCTGACCTGGCATGGATGAAGGGTGTCGGGTGGCTGACGGAGGGGAGTCTCAACCTG GAGCAGGCGAAGAAGGCTGGACAGCTCGTCAGCGAGAAACACTACCGGCAGAAGGTTGACGAGCTAAAGT TCACCAGTGTGACTGACAGCTCCCAGATGGAGCACGCCAAGAAGAGCCAGGAGCTGCAGAATGCGGTGGC CTACAAGGCAGGAAACGAGCAGTCCGTCCATCAGTACACCATCAGCAAAGACGAACCCTTCTTCCTACGA GCCCGGGCCAATGCTGCTCAACTCAGCGAGACCCTGTACAAGAGCAGCTGGGAAAAGCAGAAGGCAAAAG GATTTGAGCTGAGACTTGACTCCTTGACATTCCTGACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAGGT GAAGTACAAGGAGGACTATGAAAGGTCCAGAGGGAAACTCATCGGGGCGAAGAGCGCACAGGGGGATTCG CAGATGAGCCACTCGCTGCAGATGTCTAAGCTACAGAGTGAACTGGAGTACAAGAAGGGCTTTGAAGATA CCAGATCTCAGTACCACGTCTCCCTCGACATGATTCACCTCGCCCATGCTCGCCAGGCGCAGCACTTAGC CACAGATGTAGGCTACAGGACGGCGTCGCACTGTTTCACAGCTCTGCCCACAGACATGAAGGTGGAGTGG GCCAAGAAGGCCTACAGCCTGCAGAGTGATAACCAATACAGGGCAGACATGAAGTGGATGAAAGGCACAG GCTGGGTGGCCACCGGGTCATTAAATGTGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAA GTACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTCCAGGCCAGG ATTAGTTATACCCAAGCAGTGGATAGACTATACAGGGAGCAAGGAGAGAACTTAAAGCACCATTACACGC AGACTGTAGACTTGCCCGAGGTCCTGCTGGCCAAGCTGAACGCCATGAACATCAGCGAGACTCGCTATAA GGAGTCTTGGAGCCGGCTTCGAGACGGCGGGTATAAGCTGAGGCTGGATGCCCTTCCGTTCCAGGCAGCA AAGGCGTCCAGTGAAGTTATAAGTGACTACAAATACAAAGAAGCATTTGAGAGAATGAAAGGGCAGCTGC TCGGCTCCCGGAGCTTGGAAGATGACCTCAGCCTTGCACATTCAGTACACGCGACCTCACTGCAGAGTGA TGTGAATTATAAGAAAGGCTTTGAACACTCAAAGGCTCACTTCCATCTGCCGCTGGATATGGTAACCTTG GTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAACCAGGACTACAGACACCCGCTCCCCCAACACACAT CCTTGGCAGAAGACCTGAGGCTGCGCTGTGCCAAGAAAGCTCACAAGTTACAGAGTGAGAATCTGTACCG ATCGGACTTGAACTTCATGCGTGGCGTTCCCTGTGTCATTCCTGGAGCACTGGAGATTGAAGGAAGAAAG AAAGCCTCTGAGCTCATCAGTGAGAGTAAATACCGCCAGCATCCTGGGTCATTCAAGTACACAGCTGTGA CAGACACTCCCAACCTTCTGCATGCCAAGTTCAGCAACCAGATCACCAATGAGCGTCTCTATAAAGCAGC TGGTGAGGATGCGCGGCACCAATACACGATGACCCTTGGTCTGCCTGAATTCATCCGAGCCAAAACAAAC GCAGCCAACCTGAGCGAGGCAAAATACAAGGAGTCTTGGCATAATCTTCGTGCTCAAGGCTACAAGCTAA CGATAGACGCCCTCCCTTTCCAGGCTGCGCGGGCCTCTGGAGACATAGCCAGTGACTTTCTCTACAGGCA CGAGTTTGTGAAGGAGCGCGGGCAGCTCATTGGGGTGCGGAGTGTGAGTGACGACCCACGGCTGCGGCAC TGCCAACGGATGGGCCAGCTGCAGAGTGAGAATTGGTACAGGAAGGAGGCGGCCAGCAGCCAAGCTCAGT ACCACCTGCCCATGGATATGGTGCCCCTGGTCCATGCCAGGAAGGCCCAGGCCCTGGCTAGTGACCACGA CTACCGGACACAGTGCCATGAGTTCACCGCGCTGCCTGAGGATCTGAAGATGGCCTGGGCCAAGAAAGCG CATGCCCTGCAGAGTGAGTTTCGCTATAAAGCGGACTTGATGGGCATGAAAGGAAGTGGATGGCTGGCTC TGCAATCTCCACAGATAGAGAGTGCAAAGAAGGCTGGAGAGCTCATCAGTGAGACCAAGTACCGTGAAAA ACCAGACAGCATCAAGTTCACTACGGTGGTTGATTCTCCAGACCTCGTTCACGCCAGGAAGAGCTACGAG CACTGTAACGAGCGCCTGTACAGGTTGGGAGATGCAGACTCTCTACATAGGTACACCCCAATCGCAGACC ATCCCGATTTCACCCGAGCCCGCTTGAACGCAATGCATCTGAGTGACAAAGTCTACAGAAATTCCTGGGA GAAGAACCGGGCTGGCGGCTATGACTTCAGGCTGGATGCTATCCCGTTCCAGACTGCACGGGCATCCAGG GATATTGCCAGTGATTTCCGGTACAAAGAGGCATTTCTGAGGGACCGGGGCCTACAGATTGGGTATCGCA GCGTCAATGACGACCCAAGGATGAGACATTTCCTCCGAGTCGGCAGGCTCCAAAGTGACAATGAGTACAG AAAGGACTTCGCCAAAGGTCGCTCCCAGTTCCACAGTCGTCCTGACCAACCTGGCTTCCTCCAGGCCAAG AGGAGCCAGCAACTGGCCAGTGATGTGCTCTACAGGCAGCCACTGCCCCAGCACACCAGCGACCCAGAAG AGCTGGGCCTGAAGCACGCCCGGAAGGCTCATCAGCTACAGAGTGATGTCAAGTACAAATCTGACTTGAA CCTGACTCGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGTCGGGCTGCGGAA CTGGCCAACAGGAGGGGTCTGGGGATCCAGGGGGCTTCTGTGGAAACAGAGGCAGTGGAGCTTGGAGATC ATCGGAGCAGGGGAGTGAACCCTGATGCCTCGGAGATCCTACATATCAACAGGAAGAAGACTCTGCTGAT GTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001113743
Insert Size	5184 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001113743.1, NP_001107215.1
RefSeq Size	5483 bp
RefSeq ORF	5184 bp
Locus ID	307982

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