Wls (NM_199408) Rat Untagged Clone
CAT＃: RN200060
Wls (untagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug)
CNY 6940.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gpr177 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200060 representing NM_199408
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGCAATTATAGAAAACATGAGCACCAAGAAGCTCTGCATTGTTGGAGGGATTCTTCTGGTTT TCCAAATCGTTGCCTTTCTGGTGGGAGGCTTGATCGCTCCAGCACCCACAACGGCAGTGTCCTACGTGGC AGCAAAATGTGTGGATGTCCGGAAGAACCACCATAAAACAAGATGGCTGATGCCCTGGGGACCAAACAAG TGTAACAAGATCAATGACTTCGAAGAAGCAATTCCAAGGGAAATTGAAGCGAATGACATTGTGTTTTCTG TACACATTCCCCTCCCTTCTATGGAGATGAGCCCATGGTTCCAGTTTATGCTGTTTATCCTGCAGATAGA CATTGCTTTCAAGCTAAACAACCAAATCAGAGAAAATGCAGAAGTTTCCATGGATGTTTCCCTGGGTTAC CGTGATGATATGTTTTCTGAGTGGACTGAAATGGCGCACGAAAGAGTACCACGTAAACTCAGATGCACTT TCACATCCCCCAAGACCCCAGAGCATGAAGGTCGTCATTATGAATGTGATGTCCTTCCTTTCATGGAAAT TGGGTCAGTGGCTCATAAGTATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAGAAGAAAATCAAT GTTGGAATTGGGGAAATAAAGGACATTCGGTTGGTGGGAATCCACCAAAATGGAGGTTTCACTAAGGTAT GGTTTGCTATGAAGACCTTCCTCACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGAAGGATCAC CATGATGTCCCGACCTCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATC AATATCCCTGTGGAATGGTTTTCCATTGGATTTGATTGGACCTGGATGCTGTTATTTGGTGACATACGAC AGGGCATCTTCTATGCAATGCTTCTTTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACA TGAGCGGAATCACATTGCAGGGTATTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCTTTCTGCCTCTTC ATATTTGACATGTGTGAGAGAGGAGTGCAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTG GAACAGAACTGGCTATGGCTTTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTACTTCCTGTTTCTGTG TTTCATGGTATTTCAAGTATTCAGAAACATCAGTGGGAAACAGTCTAGCCTCCCAGCCATGAGCAAAGTC CGGAGGCTGCACTATGAGGGTCTGATTTTCAGGTTCAAGTTCCTCATGCTGATCACCTTGGCTTGTGCTG CCATGACTGTTATCTTCTTCATTGTTAGTCAGGTGACAGAAGGCCATTGGAAATGGGGTGGGGTCACAGT TCAAGTGAGCAGTGCTTTCTTCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTC TTGTATGCACCATCCCATAAGAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGG AAGAACTGCAGCTCACTACCACAATCACCCATGTAGATGGACCGACTGAGATCTACAAGTTGACCCGTAA AGAAGCACAGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199408 |
| Insert Size | 1626 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199408.2, NP_955440.1 |
| RefSeq Size | 2899 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 362065 |
| UniProt ID | Q6P689 |
| Gene Summary | Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200060 | Wls (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) |
CNY 6040.00 |
|
| RR200060L3 | Lenti ORF clone of Wls (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) |
CNY 8840.00 |
|
| RR200060L4 | Lenti ORF clone of Wls (mGFP-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) |
CNY 9690.00 |
