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SIK3 (NM_001281749) Human Tagged ORF Clone
CAT＃: RG240079
- TrueORF®
SIK3 (tGFP-tagged) - Human SIK family kinase 3 (SIK3), transcript variant 3
ORF Plasmid: DDK
"NM_001281749" in other vectors (2)
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CNY 12,920.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | L19; QSK; SEMDK; SIK-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240079 representing NM_001281749.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGGGACTGGGGGAGCCGGGCCCGCG GGCCGCCTGCTGCCTCCGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGC CAGCCGCGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTACGAGATCGAC CGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTT GCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAA ATTATGAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATT TATCTGGTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCA GAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACATT GTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGATTTT GGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCA CCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTC TACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTG AGTGGAAAGTTCCGCATCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTG TTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGGACGCC GATCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTG AATGAGGATGTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGGCGGAGCAG GCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTGGAG CCGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTAT TTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAG CTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTGAACTTC ATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGCAGTCTCTC CTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGATGGAGGA GCCAACATCCAACTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATG ACACCAGCAGTGCCAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAGGAAGCT GTGCAGAGGTACTTGGCAAATAGGTCCAAAAGACATACACTGGCCATGACCAACCCTACAGCTGAGATC CCACCGGACCTACAACGGCAGCTAGGACAGCAGCCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTA CCTGATCAGCATCGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCT GTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAAC AACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATTGAT GAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACCAGCAGGAGCAGCACCATCAAATTCTC CAGCAACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAAT CAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAACCAC CCCAACAACCATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCT CACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAACCT GAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAG CAGGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCC AGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGTACCAACCTGATG GCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTCACAGT GCACATCAGCAGCCGCCACACTATACCACGTCGGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCA GACTATACAAGACACCAGCAGGTACCCCACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACC GGCCACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGGCAGCAGCAACAACGG CAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATACCAGGAACTGTTCAGGCACATGAACCAAGGGGAT GCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGCATGACAGAGCGCCAGGCTTTATCTTATCAAAAT GCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTACAAATCAGGGCACAAGAATGTGTCTCA CAGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATG GAGGAGGACTGCTCGTGTGAGGGGGCCAAGGATGGCTTCCAAGACAGTAAGAGTTCAAGTACATTGACC AAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTGGACCTGGGGACCCTGAATCTTTGCTAGGA ACTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAA AATAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGAAGTTCTCCAGGACAAGCAGTG GAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCCTCCGTCCATGAGCACCACAGGCCCCGG GCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTATGTACAGCTGGATAACTTGCCAGGA ATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATGTCGGATGCAGTTCTCAGTCAGTCTTCG CTCATGGGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAG CACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCTACGTGTATTACAGAC ATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAGGCAGGCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240079
Blue=ORF Red=Cloning site Green=Tag(s) MAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEID RTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMI YLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADF GFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVL SGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPL NEDVLLAMEDMGLDKEQTLQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRY LSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSL LQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEA VQRYLANRSKRHTLAMTNPTAEIPPDLQRQLGQQPFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSP VRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQIL QQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQP HGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGS SGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSAHQQPPHYTTSALQQALLSPTPP DYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGD AGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESM EEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSK NKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPG MSLVAGKALSSARMSDAVLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITD ILLSYKHPEVSFSMEQAGV TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001281749 |
| ORF Size | 3783 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001281749.3 |
| RefSeq Size | 6039 bp |
| RefSeq ORF | 3786 bp |
| Locus ID | 23387 |
| UniProt ID | Q9Y2K2 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 139 kDa |
| Gene Summary | Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed:30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
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