POM121 (NM_001257190) Human Tagged ORF Clone
CAT＃: RG235113
- TrueORF®
POM121 (tGFP-tagged) - Homo sapiens POM121 transmembrane nucleoporin (POM121), transcript variant 1
ORF Plasmid: DDK
"NM_001257190" in other vectors (2)
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CNY 9648.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | P145; POM121A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001257190 |
| ORF Size | 2997 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001257190.2, NP_001244119.1 |
| RefSeq Size | 7041 bp |
| RefSeq ORF | 3000 bp |
| Locus ID | 9883 |
| UniProt ID | Q96HA1 |
| Gene Summary | This gene encodes a transmembrane protein that localizes to the inner nuclear membrane and forms a core component of the nuclear pore complex, which mediates transport to and from the nucleus. The encoded protein may anchor this complex to the nuclear envelope. There are multiple related genes and pseudogenes for this gene on chromosomes 5, 7, 15, and 22. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD
,Coyne, AN;Zaepfel, BL;Hayes, L;Fitchman, B;Salzberg, Y;Luo, EC;Bowen, K;Trost, H;Aigner, S;Rigo, F;Yeo, GW;Harel, A;Svendsen, CN;Sareen, D;Rothstein, JD;,
Neuron
,PubMed ID 32673563
[POM121]
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Documents
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Resources
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