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LONP1 (NM_001276480) Human Tagged ORF Clone
CAT＃: RG234853
- TrueORF®
LONP1 (tGFP-tagged) - Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3
ORF Plasmid: DDK
"NM_001276480" in other vectors (2)
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CNY 6,370.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234853 representing NM_001276480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGACCTTGGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGC TGGAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAGCGGGGCAA GAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATGGAGCCCACCCCTGAGCTC CCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCACGAGGACTTCCAGGTCACGGAGGAGGTGA AAGCCCTGACTGCAGAGATCGTGAAGACCATCCGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTC AGTGCTGCAGATGATGCAGGCTGGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCC GCGCTCACCGGGGCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGT ACAAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGGCGGGAGGT GGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTAAAGATCATCAAGAAGGAG CTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAGGAGCTCGTGG TCCCCAAGCACGTCATGGATGTTGTGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTC GGAGTTCAATGTCACCCGCAACTACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAG AACCTGGACCTGGCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAAC GCATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGCTTCTATGG CCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTGAACCGAGAGTACTTCCGC TTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGCCACAGGCGGACCTACGTGGGCGCCATGC CCGGGAAGATCATCCAGTGTTTGAAGAAGACCAAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGA CAAGATCGGCCGAGGCTACCAGGGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAAT GCCAACTTCCTGGACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCA ACGTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGCTACGTGGC CCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGCGCCCTGTGTGGCTTGGATGAG AGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATCAAGCAGTACTGCCGCGAGAGCGGTGTCC GCAACCTGCAGAAGCAAGTGGAGAAGGTGTTACGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGA GTCCGTGGAGGTGACGCCCGAGAACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATG TATGACGTGACACCGCCCGGCGTGGTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTG TGGAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTGGAGGTGAC AGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCCTACACCTTCGCCAGAGCCTTCCTCATG CAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATCCACCTGCATGTGCCCGAGGGCGCCACCC CCAAGGACGGCCCAAGCGCAGGCTGCACCATCGTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGT CCGGCAGAATCTGGCCATGACTGGCGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAG GAGAAGACCATTGCGGCCAAGCGCGCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACT TCTACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGGGAGATCTT CGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTGGCCGTGGAACGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234853 representing NM_001276480
Red=Cloning site Green=Tags(s) MQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPEL PAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGA ALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKE LGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNE NLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFR FSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQN ANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDE SKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERM YDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLM QHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIK EKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001276480 |
| ORF Size | 2289 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001276480.1, NP_001263409.1 |
| RefSeq Size | 2900 bp |
| RefSeq ORF | 2292 bp |
| Locus ID | 9361 |
| UniProt ID | P36776 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013] |
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