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PNPLA8 (NM_001256008) Human Tagged ORF Clone
CAT＃: RG233133
- TrueORF®
PNPLA8 (tGFP-tagged) - Homo sapiens patatin-like phospholipase domain containing 8 (PNPLA8), transcript variant 3
ORF Plasmid: DDK
"NM_001256008" in other vectors (2)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IPLA2-2; IPLA2G; iPLA2gamma; MMLA; PNPLA-gamma |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233133 representing NM_001256008
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATTAATCTGACTGTAGATATATATATTTACCTCCTTAGTAATGCAAGAAGTGTTTGTGGGAAGC AGAGAAGCAAGCAACTGTATTTCTTGTTCTCACCTAAGCATTACTGGAGGATAAGCCACATCAGTCTACA AAGAGGTTTTCATACAAACATAATAAGATGTAAATGGACCAAAAGTGAAGCACATTCTTGCAGTAAGCAC TGTTACTCTCCAAGCAACCATGGTTTACATATTGGGATTTTGAAACTTAGCACTTCTGCTCCCAAGGGAC TTACAAAAGTGAACATTTGTATGTCCCGTATTAAAAGTACTTTGAACTCTGTTTCAAAGGCTGTTTTTGG CAATCAAAATGAAATGATTTCACGTTTAGCTCAATTTAAGCCAAGTTCCCAAATTTTAAGAAAAGTATCG GATAGTGGCTGGTTAAAACAGAAAAACATCAAACAAGCCATCAAATCTCTGAAAAAATATAGTGACAAAT CAGCAGAAAAGAGTCCTTTTCCAGAAGAGAAAAGTCACATTATAGACAAAGAAGAAGATATAGGTAAACG CAGTCTTTTTCATTACACAAGTTCTATAACCACAAAATTTGGAGACTCATTCTACTTTTTATCAAATCAT ATTAATTCATATTTCAAACGTAAGGAAAAAATGTCTCAACAAAAGGAAAATGAACATTTCCGGGACAAAT CAGAACTTGAAGATAAAAAGGTAGAAGAGGGGAAATTAAGATCTCCAGATCCTGGCATCCTGGCTTATAA GCCAGGCTCAGAATCTGTACATACGGTGGACAAGCCTACAAGTCCTTCTGCGATACCTGATGTTCTTCAA GTTTCAACTAAACAAAGTATTGCTAACTTTCTTTCTCGTCCCACGGAAGGTGTACAAGCTTTAGTAGGTG GTTATATTGGTGGACTTGTCCCCAAATTAAAGTATGATTCAAAGAGTCAGTCAGAAGAACAGGAAGAGCC TGCTAAAACTGATCAGGCTGTCAGCAAAGACAGAAATGCAGAGGAGAAAAAGCGTTTATCTCTTCAGCGA GAAAAGATTATCGCAAGGGTGAGTATTGATAACAGGACCCGGGCATTAGTTCAGGCATTAAGAAGAACAA CTGACCCAAAGCTCTGCATTACTAGGGTTGAAGAACTGACTTTTCATCTTCTAGAATTTCCTGAAGGAAA AGGAGTGGCTGTCAAGGAAAGAATTATTCCATATTTATTACGACTGAGACAAATTAAGGATGAAACTCTT CAGGCTGCAGTTAGAGAAATTTTGGCCCTAATTGGCTATGTGGATCCAGTGAAAGGGAGAGGAATCCGAA TTCTCTCAATTGATGGTGGAGGAACAAGGGGCGTGGTTGCTCTCCAGACCCTACGAAAATTAGTTGAACT TACTCAGAAGCCAGTTCATCAGCTCTTTGATTACATTTGTGGTGTAAGCACAGGTGCCATATTAGCTTTC ATGTTGGGGTTGTTTCATATGCCCTTGGATGAATGTGAGGAACTTTATCGAAAATTAGGATCAGATGTAT TTTCACAAAATGTCATTGTTGGAACAGTAAAAATGAGTTGGAGCCATGCATTTTATGACAGTCAAACATG GGAAAACATTCTTAAGGATAGGATGGGATCTGCACTGATGATTGAAACAGCAAGAAACCCCACATGTCCT AAGGTAGCTGCTGTAAGTACCATAGTAAATAGAGGGATAACACCCAAAGCTTTTGTGTTCAGAAACTATG GTCATTTTCCTGGAATCAACTCTCATTATTTGGGAGGCTGTCAGTATAAAATGTGGCAGGCCATTAGAGC CTCATCTGCTGCTCCAGGCTACTTTGCAGAATATGCATTGGGAAATGATCTTCATCAAGATGGAGGTTTG CTTCTGAATAACCCTTCGGCATTAGCTATGCATGAGTGTAAATGTCTTTGGCCAGATGTGCCGTTAGAGT GCATAGTATCCCTGGGCACTGGACGTTATGAGAGTGATGTGAGAAACACGGTAACATACACAAGCTTGAA AACTAAACTTTCTAATGTTATCAACAGTGCTACAGATACAGAAGAAGTCCATATAATGCTTGATGGCCTG TTACCTCCTGACACCTATTTTAGATTCAATCCTGTAATGTGTGAAAACATACCTCTAGATGAAAGTCGAA ATGAAAAGCTGGATCAGCTGCAGTTGGAAGGGTTGAAATACATAGAAAGAAATGAACAAAAAATGAAAAA AGTTGCAAAAATATTAAGTCAAGAAAAAACAACTCTGCAGAAAATTAATGATTGGATAAAATTAAAAACT GATATGTATGAAGGACTTCCATTCTTTTCAAAATTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233133 representing NM_001256008
Red=Cloning site Green=Tags(s) MSINLTVDIYIYLLSNARSVCGKQRSKQLYFLFSPKHYWRISHISLQRGFHTNIIRCKWTKSEAHSCSKH CYSPSNHGLHIGILKLSTSAPKGLTKVNICMSRIKSTLNSVSKAVFGNQNEMISRLAQFKPSSQILRKVS DSGWLKQKNIKQAIKSLKKYSDKSAEKSPFPEEKSHIIDKEEDIGKRSLFHYTSSITTKFGDSFYFLSNH INSYFKRKEKMSQQKENEHFRDKSELEDKKVEEGKLRSPDPGILAYKPGSESVHTVDKPTSPSAIPDVLQ VSTKQSIANFLSRPTEGVQALVGGYIGGLVPKLKYDSKSQSEEQEEPAKTDQAVSKDRNAEEKKRLSLQR EKIIARVSIDNRTRALVQALRRTTDPKLCITRVEELTFHLLEFPEGKGVAVKERIIPYLLRLRQIKDETL QAAVREILALIGYVDPVKGRGIRILSIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAF MLGLFHMPLDECEELYRKLGSDVFSQNVIVGTVKMSWSHAFYDSQTWENILKDRMGSALMIETARNPTCP KVAAVSTIVNRGITPKAFVFRNYGHFPGINSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGL LLNNPSALAMHECKCLWPDVPLECIVSLGTGRYESDVRNTVTYTSLKTKLSNVINSATDTEEVHIMLDGL LPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGLKYIERNEQKMKKVAKILSQEKTTLQKINDWIKLKT DMYEGLPFFSKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256008 |
| ORF Size | 2346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256008.3 |
| RefSeq Size | 4701 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 50640 |
| UniProt ID | Q9NP80 |
| Gene Summary | This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. Mutations in this gene have been associated with mitochondrial myopathy with lactic acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC233133 | PNPLA8 (Myc-DDK tagged) - Homo sapiens patatin-like phospholipase domain containing 8 (PNPLA8), transcript variant 3 |
CNY 5,728.00 |
|
| SC330346 | PNPLA8 (untagged) - Homo sapiens patatin-like phospholipase domain containing 8 (PNPLA8), transcript variant 3 |
CNY 7,410.00 |
