TMEM127 (NM_001193304) Human Tagged ORF Clone
CAT#: RG231506
- TrueORF®
TMEM127 (tGFP-tagged) - Homo sapiens transmembrane protein 127 (TMEM127), transcript variant 2
ORF Plasmid: DDK
"NM_001193304" in other vectors (2)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231506 representing NM_001193304
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCCCCCGGAGGCGCAGGGCTGCCCGGCGGGCGCCGGCGGAGGAGCCCGGGAGGCAGCGCTCTGC CCAAGCAGCCGGAGCGTAGCCTGGCCTCGGCCCTGCCTGGCGCCCTGTCTATCACGGCGCTGTGCACTGC CCTCGCCGAGCCCGCCTGGTTGCACATCCACGGAGGCACCTGTTCGCGCCAGGAGCTGGGGGTCTCCGAC GTGTTGGGCTATGTGCACCCGGACCTGCTGAAAGATTTCTGCATGAATCCCCAGACAGTGCTGCTCCTGC GGGTCATCGCCGCCTTCTGTTTCCTGGGCATCCTGTGTAGTCTCTCCGCTTTCCTTCTGGATGTCTTTGG GCCGAAGCATCCTGCTCTGAAGATCACTCGTCGCTATGCCTTCGCCCATATCCTAACGGTTCTGCAGTGT GCCACCGTCATTGGCTTTTCTTATTGGGCTTCTGAACTCATCTTGGCCCAGCAGCAGCAGCATAAGAAGT ACCATGGATCCCAGGTCTATGTCACCTTCGCCGTTAGCTTCTACCTGGTGGCAGGAGCTGGTGGAGCCTC AATCCTGGCCACGGCAGCCAACCTCCTGCGCCACTACCCCACAGAGGAAGAGGAGCAGGCGCTGGAGCTG CTCTCAGAGATGGAAGAGAACGAGCCCTACCCGGCGGAATATGAGGTCATCAACCAGTTCCAGCCACCCC CTGCTTACACACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231506 representing NM_001193304
Red=Cloning site Green=Tags(s) MYAPGGAGLPGGRRRRSPGGSALPKQPERSLASALPGALSITALCTALAEPAWLHIHGGTCSRQELGVSD VLGYVHPDLLKDFCMNPQTVLLLRVIAAFCFLGILCSLSAFLLDVFGPKHPALKITRRYAFAHILTVLQC ATVIGFSYWASELILAQQQQHKKYHGSQVYVTFAVSFYLVAGAGGASILATAANLLRHYPTEEEEQALEL LSEMEENEPYPAEYEVINQFQPPPAYTP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001193304 |
ORF Size | 714 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193304.3 |
RefSeq Size | 4570 bp |
RefSeq ORF | 717 bp |
Locus ID | 55654 |
UniProt ID | O75204 |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a transmembrane protein with 3 predicted transmembrane domains. The protein is associated with a subpopulation of vesicular organelles corresponding to early endosomal structures, with the Golgi, and with lysosomes, and may participate in protein trafficking between these structures. Mutations in this gene and several other genes cause pheochromocytomas. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2010] |
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