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ANO6 (NM_001142680) Human Tagged ORF Clone
CAT＃: RG226670
- TrueORF®
ANO6 (tGFP-tagged) - Human anoctamin 6 (ANO6), transcript variant 4
ORF Plasmid: DDK
"NM_001142680" in other vectors (4)
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CNY 8,408.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC104751; TMEM16F |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226670 representing NM_001142680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAAGATGAGCAGGAATGTTTTGCTACAAATGGAGGAGGAGGAGGACGACGACGATGGGGATATCG TGTTGGAAAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTCAGCATGATTTTCGAAC CCCGGAGTTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGGCCAGCGAAGAATTGAC TTTGTTCTAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACAAATGAAAAACAAAGGA GGAAAAGACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAGCAACAAGATCAGTATT GGATGACAAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTACGTATGCTGAGATAATG CACATCAAATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTTGGTACACTCAACTGGT TTACCAAAGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTTTCACTGCCCCATTTGA GAAGAACCGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCCAGCCACCAGAAGCCGC ATTGTTTACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGCAAGTTTGGGATCAACA GACTTGTAAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAATTCCGCCGTCAGTCAGA GGATCCCAGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCCTCGAAGCATATACAAA AAGCAGCCCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTACTTTGCTTGGCTGGGCT ATTACACTCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCTATGGATATCTTAATCA AGATAACTGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGATCATAATGTGTCCTCAG TGTGATAGGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAGAAATTGTGCATCTTCG ACAGTTTTGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTGTTTTTGGAGTTTTGGAA GCGACGCCAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAGGAAGAACAAGCCCGACCA GAATACGAAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAAGAAGAACGCATTCCCTTTA CTGCCTGGGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTTTTCTGGATCCTATTGATCAT CGCTTCAGTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTATTTTCTGCAAAACTTCCCAAG AACATTAATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACAGCCACGTCCATCACGGCCTCCA TCATCAGCTTTATAATTATCATGATTCTGAACACCATATATGAAAAAGTGGCAATTATGATTACTAACTT CGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATGAAGATGTTCTTATTCCAGTTTGTC AACTACTACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAATTTGTAGGCTATCCAGGAGACCCAG TTTATTGGTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGTGGCTGTCTTCTTGAACTGACAACTCA GCTGACAATAATCATGGGAGGAAAAGCAATCTGGAATAACATACAAGAAGTATTATTGCCCTGGATCATG AATCTAATTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAGATAACCCCACGATGGGAACAGGACTACC ATCTGCAGCCTATGGGCAAACTGGGATTATTTTATGAATATCTTGAAATGATTATTCAGTTTGGGTTCGT CACCTTATTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTGGCTCTCGTGAACAATATATTGGAAATAAGA GTGGACGCATGGAAACTGACCACCCAGTTTAGACGCCTGGTACCAGAGAAAGCCCAAGACATTGGAGCAT GGCAGCCCATCATGCAAGGAATAGCAATTCTGGCTGTGGTGACCAATGCCATGATCATAGCTTTCACGTC GGACATGATCCCCCGCCTAGTGTACTACTGGTCCTTCTCCGTCCCTCCCTACGGGGACCACACTTCCTAC ACCATGGAAGGGTACATCAACAACACTCTCTCCATCTTCAAAGTCGCAGACTTCAAAAACAAAAGCAAGG GAAACCCGTACTCTGACCTGGGTAACCATACCACATGCAGGTATCGTGATTTCCGATACCCACCTGGACA CCCCCAGGAGTATAAACACAACATCTACTATTGGCATGTGATTGCAGCCAAGCTGGCTTTTATCATTGTC ATGGAGTATCTCGCTTTGTTGCCCAGGCTGGGACACAGTGGCATGATCTTGGCTCACTGCAACCTCCGCC TCCCAGTTGACTGCTGTATGTGTTATAGGTTTGTCGATGAAATTAGACTTTTGGAACAACTAACTTCTGA CTTTATTGACTCCCTCTATTATATATTTTCTATTTCAATAATATCTATTTTCTTTTCTGTTACTTTCTTT TTCCTTCTACTTTCTTTGGGGCCAACTCCGTGTTTTTCTGTATCGAATTTCTTAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226670 representing NM_001142680
Red=Cloning site Green=Tags(s) MKKMSRNVLLQMEEEEDDDDGDIVLENLGQTIVPDLGSLESQHDFRTPEFEEFNGKPDSLFFNDGQRRID FVLVYEDESRKETNKKGTNEKQRRKRQAYESNLICHGLQLEATRSVLDDKLVFVKVHAPWEVLCTYAEIM HIKLPLKPNDLKNRSSAFGTLNWFTKVLSVDESIIKPEQEFFTAPFEKNRMNDFYIVDRDAFFNPATRSR IVYFILSRVKYQVINNVSKFGINRLVNSGIYKAAFPLHDCKFRRQSEDPSCPNERYLLYREWAHPRSIYK KQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLNQDNCTWSKEVCHPDIGGKIIMCPQ CDRLCPFWKLNITCESSKKLCIFDSFGTLVFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQARP EYEARCTHVVINEITQEEERIPFTAWGKCIRITLCASAVFFWILLIIASVIGIIVYRLSVFIVFSAKLPK NINGTDPIQKYLTPQTATSITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQFV NYYSSCFYIAFFKGKFVGYPGDPVYWLGKYRNEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWIM NLIGRFHRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEIR VDAWKLTTQFRRLVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSVPPYGDHTSY TMEGYINNTLSIFKVADFKNKSKGNPYSDLGNHTTCRYRDFRYPPGHPQEYKHNIYYWHVIAAKLAFIIV MEYLALLPRLGHSGMILAHCNLRLPVDCCMCYRFVDEIRLLEQLTSDFIDSLYYIFSISIISIFFSVTFF FLLLSLGPTPCFSVSNFLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142680 |
| ORF Size | 2787 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142680.1, NP_001136152.1 |
| RefSeq Size | 3448 bp |
| RefSeq ORF | 2789 bp |
| Locus ID | 196527 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226670 | ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 4 |
CNY 6,808.00 |
|
| RC226670L3 | Lenti ORF clone of Human anoctamin 6 (ANO6), transcript variant 4, Myc-DDK-tagged |
CNY 11,210.00 |
|
| RC226670L4 | Lenti ORF clone of Human anoctamin 6 (ANO6), transcript variant 4, mGFP tagged |
CNY 11,210.00 |
|
| SC326290 | ANO6 (untagged)-Human anoctamin 6 (ANO6), transcript variant 4 |
CNY 17,860.00 |
