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MACC1 (NM_182762) Human Tagged ORF Clone
CAT＃: RG224774
- TrueORF®
MACC1 (tGFP-tagged) - Human metastasis associated in colon cancer 1 (MACC1)
ORF Plasmid: DDK
"NM_182762" in other vectors (6)
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CNY 10,960.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 7A5; SH3BP4L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224774 representing NM_182762
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAATCACTGAAAGAAAACATTTTCGGTCAGGAAGAATTGCACAAAGTATGTCTGAAGCAAATTTGA TTGACATGGAAGCTGGAAAAGTCTCAAAAAGTTGCAATATTACAGAATGCCAGGACCCAGACTTGCTTCA CAATTGGCCGGATGCTTTCACCCTTCGTGGTAATAATGCTTCCAAAGTTGCAAATCCATTCTGGAATCAA CTGTCTGCTTCTAACCCATTTTTGGATGACATAACTCAACTAAGAAATAACAGGAAGAGAAATAATATTT CCATCTTAAAGGAAGATCCTTTTCTTTTCTGTAGAGAAATAGAAAATGGAAACTCTTTTGATTCCTCCGG TGATGAACTTGATGTGCATCAGTTACTTAGGCAGACTTCCTCAAGAAATTCTGGAAGATCTAAAAGTGTT TCAGAACTTCTGGACATTTTAGACGACACAGCACATGCCCATCAGAGTATACATAACTCTGACCAGATCC TACTACACGACTTAGAGTGGCCTAAAAATGATCGGGAGGCTTATAAAATGGCTTGGTTAAGTCAACGCCA GCTGGCCCGCTCCTGCCTTGATTTGAATACAATTAGTCAGAGCCCTGGATGGGCCCAGACACAACTTGCG GAGGTCACCATAGCTTGCAAAGTAAACCATCAAGGAGGGTCAGTACAATTACCTGAATCAGACATCACTG TTCATGTGCCCCAAGGTCATGTGGCTGTGGGAGAATTCCAAGAGGTGTCTCTAAGGGCTTTCCTTGATCC GCCACACATGCTTAACCATAATCTTTCGTGCACTGTGAGCCCGTTGTTGGAAATCATGTTAGGCAACCTC AATACAATGGAAGCCCTTTTGCTGGAGATGAAAATTGGGGCTGAAGTAAGAAAGGATCCTTTCAGCCAAG TCATGACAGAAATGGTGTGTTTACACAGCTTGGGTAAAGAAGGCCCTTTTAAAGTTTTAAGCAACTGCTA CATTTATAAAGACACCATCCAAGTCAAGCTAATCGACTTGAGTCAGGTAATGTATCTAGTGGTTGCTGCA CAAGCTAAAGCTCTTCCGTCACCAGCTGCCACCATTTGGGATTATATCCACAAAACCACCTCAATTGGAA TTTATGGACCCAAATATATCCATCCCAATTTTACTGTTGTTTTAACAGTTTGTGGACACAATTATATGCC AGGACAGCTTACAATTTCTGATATTAAGAAGGGTGGAAAAAACATATCTCCAGTTGTGTTTCAGCTCTGG GGGAAGCAGTCATTTTTACTTGACAAGCCACAAGATTTAAGTATTTCTATTTTTTCCTGTGATCCTGATT TTGAAGTAAAGACAGAAGGAGAAAGGAAAGAAATTAAACAAAAGCAGTTGGAAGCAGGTGAAGTAGTTCA TCAACAATTTTTATTTTCTTTAGTTGAGCACAGAGAGATGCACTTGTTTGATTTTTGTGTTCAAGTGGAG CCTCCCAATGGTGAACCAGTTGCACAGTTCTCTATCACTACTCCTGATCCAACCCCAAACCTAAAAAGAC TCTTGAATCTGCCAGGCTATTTGCAGAAGAAGGAGGAAATCAAGTCTGCTCCTTTATCACCAAAAATTCT TGTTAAATATCCTACATTTCAAGATAAAACATTGAACTTTAGCAACTATGGGGTAACCCTGAAGGCAGTG CTAAGACAAAGCAAGATTGATTACTTCCTTGAATATTTCAAAGGGGACACAATAGCTCTCCTCGGGGAAG GTAAGGTAAAAGCTATTGGTCAGTCCAAAGTGAAAGAATGGTATGTAGGAGTCCTCAGAGGTAAGATTGG ACTTGTACACTGCAAAAATGTCAAGGTGATTTCAAAGGAGCAAGTAATGTTTATGTCAGATAGTGTCTTT ACAACCAGAAATCTTCTTGAACAGATTGTCCTGCCTTTAAAAAAATTGACTTATATCTACTCAGTTGTAT TAACCTTGGTGTCAGAAAAAGTTTATGATTGGAAAGTTTTAGCTGATGTCCTGGGTTACTCACATCTGTC CCTGGAAGATTTTGATCAAATTCAAGCAGACAAAGAATCAGAGAAAGTTTCTTATGTTATAAAGAAGTTA AAGGAAGATTGCCACACAGAGAGAAATACAAGGAAGTTTCTGTATGAACTTATTGTGGCTCTTCTGAAAA TGGATTGCCAAGAGTTAGTCGCACGTCTCATCCAAGAAGCTGCTGTTCTGACTTCAGCTGTCAAGCTTGG AAAAGGCTGGAGGGAACTAGCTGAAAAGTTAGTACGACTCACAAAGCAACAAATGGAGGCATATGAAATT CCTCATCGAGGAAACACTGGAGATGTTGCTGTTGAGATGATGTGGAAACCTGCCTATGATTTTCTGTATA CCTGGAGTGCTCACTATGGAAATAACTACACAGATGTGTTACAAGACCTTCAGTCAGCTTTGGACAGAAT GAAAAACCCTGTGACTAAACACTGGAGAGAATTAACTGGAGTTTTAATACTAGTAAATTCTTTGGAGGTT TTGAGAGTAACTGCATTCTCCACTTCTGAGGAAGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224774 representing NM_182762
Red=Cloning site Green=Tags(s) MLITERKHFRSGRIAQSMSEANLIDMEAGKVSKSCNITECQDPDLLHNWPDAFTLRGNNASKVANPFWNQ LSASNPFLDDITQLRNNRKRNNISILKEDPFLFCREIENGNSFDSSGDELDVHQLLRQTSSRNSGRSKSV SELLDILDDTAHAHQSIHNSDQILLHDLEWPKNDREAYKMAWLSQRQLARSCLDLNTISQSPGWAQTQLA EVTIACKVNHQGGSVQLPESDITVHVPQGHVAVGEFQEVSLRAFLDPPHMLNHNLSCTVSPLLEIMLGNL NTMEALLLEMKIGAEVRKDPFSQVMTEMVCLHSLGKEGPFKVLSNCYIYKDTIQVKLIDLSQVMYLVVAA QAKALPSPAATIWDYIHKTTSIGIYGPKYIHPNFTVVLTVCGHNYMPGQLTISDIKKGGKNISPVVFQLW GKQSFLLDKPQDLSISIFSCDPDFEVKTEGERKEIKQKQLEAGEVVHQQFLFSLVEHREMHLFDFCVQVE PPNGEPVAQFSITTPDPTPNLKRLLNLPGYLQKKEEIKSAPLSPKILVKYPTFQDKTLNFSNYGVTLKAV LRQSKIDYFLEYFKGDTIALLGEGKVKAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMFMSDSVF TTRNLLEQIVLPLKKLTYIYSVVLTLVSEKVYDWKVLADVLGYSHLSLEDFDQIQADKESEKVSYVIKKL KEDCHTERNTRKFLYELIVALLKMDCQELVARLIQEAAVLTSAVKLGKGWRELAEKLVRLTKQQMEAYEI PHRGNTGDVAVEMMWKPAYDFLYTWSAHYGNNYTDVLQDLQSALDRMKNPVTKHWRELTGVLILVNSLEV LRVTAFSTSEEV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182762 |
| ORF Size | 2556 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182762.2, NP_877439.2 |
| RefSeq Size | 3188 bp |
| RefSeq ORF | 2559 bp |
| Locus ID | 346389 |
| UniProt ID | Q6ZN28 |
| Gene Summary | MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).[supplied by OMIM, Mar 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The newly identified MEK1 tyrosine phosphorylation target MACC1 is druggable by approved MEK1 inhibitors to restrict colorectal cancer metastasis
,null,
Oncogene
,PubMed ID 34247190
[MACC1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224774 | MACC1 (Myc-DDK-tagged)-Human metastasis associated in colon cancer 1 (MACC1) |
CNY 9,360.00 |
|
| RC224774L1 | Lenti ORF clone of Human metastasis associated in colon cancer 1 (MACC1), Myc-DDK-tagged |
CNY 11,760.00 |
|
| RC224774L2 | Lenti ORF clone of Human metastasis associated in colon cancer 1 (MACC1), mGFP tagged |
CNY 11,760.00 |
|
| RC224774L3 | Lenti ORF clone of Human metastasis associated in colon cancer 1 (MACC1), Myc-DDK-tagged |
CNY 11,760.00 |
|
| RC224774L4 | Lenti ORF clone of Human metastasis associated in colon cancer 1 (MACC1), mGFP tagged |
CNY 11,760.00 |
|
| SC307508 | MACC1 (untagged)-Human metastasis associated in colon cancer 1 (MACC1) |
CNY 9,368.00 |
