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THOC5 (NM_003678) Human Tagged ORF Clone
CAT＃: RG223375
- TrueORF®
THOC5 (tGFP-tagged) - Human THO complex 5 (THOC5), transcript variant 4
ORF Plasmid: DDK
"NM_003678" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C22orf19; Fmip; fSAP79; PK1.3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223375 representing NM_003678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCAGAATCGAGCAAAAAACGGAAGCCCAAAGTGATCCGAAGCGATGGAGCCCCAGCTGAAGGAA AGCGGAATCGATCTGACACCGAGCAGGAAGGTAAATACTACAGTGAGGAGGCCGAGGTGGATCTGCGGGA CCCTGGCAGAGACTATGAGTTATACAAGTACACCTGCCAGGAGCTACAGAGGCTGATGGCTGAGATCCAA GACCTGAAGAGCAGGGGTGGCAAGGATGTGGCAATAGAAATAGAAGAACGGAGGATCCAGAGCTGTGTGC ATTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCACATCAGGTTGAAGAAAGGAAGAGATCAGACCCA CGAGGCTAAGCAGAAAGTAGATGCCTATCATCTGCAGCTCCAGAACCTGTTGTATGAGGTGATGCACCTA CAGAAGGAGATCACCAAATGTTTGGAGTTTAAGTCAAAGCATGAAGAAATTGATCTGGTCAGTTTAGAGG AGTTTTATAAGGAGGCTCCACCAGATATCAGCAAGGCCGAAGTCACCATGGGAGACCCTCACCAGCAAAC ACTGGCACGTCTGGACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAGAAGTACCGAGAGTGCCTATCT AACAAGGAGAAGATTCTCAAGGAGATTGAGGTGAAGAAGGAGTACCTGAGCAGCCTCCAGCCCCGCCTCA ACAGCATCATGCAGGCTTCCCTTCCGGTGCAGGAGTACCTGTTTATGCCATTCGACCAGGCTCACAAGCA GTATGAGACAGCCAGACACCTGCCGCCTCCCCTCTATGTCCTCTTTGTTCAGGCCACTGCGTATGGGCAG GCCTGTGATAAGACGTTATCTGTGGCAATCGAAGGCAGTGTGGATGAAGCCAAGGCTCTGTTCAAACCTC CAGAGGACTCCCAAGATGACGAGAGTGACTCAGATGCCGAGGAGGAGCAGACTACGAAGCGCCGGAGACC CACACTGGGGGTTCAGTTGGACGACAAACGCAAGGAGATGCTGAAGAGGCACCCACTGTCTGTCATGCTC GACCTGAAGTGCAAAGATGACAGTGTGCTTCACCTGACTTTCTACTACCTCATGAACCTCAACATCATGA CAGTAAAAGCCAAAGTGACAACTGCCATGGAGCTGATCACCCCCATCAGTGCAGGTGACTTGCTGTCTCC TGACTCAGTCCTGAGTTGCTTGTATCCTGGGGATCATGGAAAGAAAACTCCGAATCCAGCCAATCAGTAT CAGTTTGATAAAGTTGGCATCCTGACTTTGAGCGACTATGTACTTGAGCTAGGTCACCCCTATTTGTGGG TGCAGAAGCTGGGTGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCAAACAGTGATTGCTGACCACTCGCT GAGCGCCAGCCACATGGAGACCACCATGAAACTTCTGAAGACCAGGGTGCAGTCCCGCCTGGCCCTCCAC AAACAGTTTGCATCCCTAGAACATGGCATTGTGCCAGTTACCAGTGATTGCCAGTACCTCTTCCCTGCCA AGGTTGTCTCTCGCCTGGTGAAATGGGTGACAGTTGCCCATGAGGATTACATGGAGCTGCACTTCACCAA AGACATTGTGGATGCGGGACTGGCTGGGGACACCAATCTCTACTACATGGCGCTCATCGAAAGGGGCACA GCCAAACTGCAGGCCGCTGTGGTGTTGAACCCTGGCTACTCCTCCATCCCACCTGTTTTCCAGCTCTGTT TGAACTGGAAAGGGGAGAAAACCAACAGCAACGATGACAACATTCGGGCCATGGAGGGCGAAGTCAATGT GTGCTACAAGGAGCTGTGTGGCCCTTGGCCCAGCCACCAGCTGTTGACCAACCAGCTGCAGCGGCTGTGT GTGCTGCTGGATGTTTACCTGGAGACCGAGAGCCATGACGACAGTGTGGAGGGGCCCAAGGAATTTCCCC AGGAGAAGATGTGTCTGCGGCTCTTCAGGGGTCCTAGCAGGATGAAGCCATTTAAATACAACCATCCTCA GGGATTCTTCAGCCATCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223375 representing NM_003678
Red=Cloning site Green=Tags(s) MSSESSKKRKPKVIRSDGAPAEGKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQELQRLMAEIQ DLKSRGGKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYHLQLQNLLYEVMHL QKEITKCLEFKSKHEEIDLVSLEEFYKEAPPDISKAEVTMGDPHQQTLARLDWELEQRKRLAEKYRECLS NKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQ ACDKTLSVAIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVML DLKCKDDSVLHLTFYYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQY QFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSASHMETTMKLLKTRVQSRLALH KQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLC VLLDVYLETESHDDSVEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003678 |
| ORF Size | 2049 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003678.5 |
| RefSeq Size | 2447 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 8563 |
| UniProt ID | Q13769 |
| Gene Summary | Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223375 | THOC5 (Myc-DDK-tagged)-Human THO complex 5 (THOC5), transcript variant 4 |
CNY 3,990.00 |
|
| RC223375L3 | Lenti-ORF clone of THOC5 (Myc-DDK-tagged)-Human THO complex 5 (THOC5), transcript variant 4 |
CNY 5,890.00 |
|
| RC223375L4 | Lenti-ORF clone of THOC5 (mGFP-tagged)-Human THO complex 5 (THOC5), transcript variant 4 |
CNY 5,890.00 |
|
| SC117857 | THOC5 (untagged)-Human THO complex 5 (THOC5), transcript variant 4 |
CNY 2,950.00 |
