COG5 (NM_181733) Human Tagged ORF Clone
CAT＃: RG220704
- TrueORF®
COG5 (tGFP-tagged) - Human component of oligomeric golgi complex 5 (COG5), transcript variant 2
ORF Plasmid: DDK
"NM_181733" in other vectors (4)
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CNY 9690.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDG2I; GOLTC1; GTC90 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220704 representing NM_181733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGGTGGGCGGGCGGCGCCGGGATTCTGCGTCACCACCTGGGCGGAGCCGTTCTGCTGCTGACG ACATCAACCCGGCACCTGCCAACATGGAAGGTGGCGGCGGCAGCGTCGCTGTAGCTGGCCTCGGAGCTCG AGGCTCTGGAGCGGCTGCAGCTACAGTCCGGGAACTTCTGCAGGACGGGTGTTATAGTGACTTTTTAAAC GAAGACTTTGATGTAAAGACTTATACTTCTCAATCTATTCATCAAGCTGTAATTGCTGAACAACTAGCAA AACTTGCCCAAGGAATCAGTCAGTTGGACAGAGAACTACACTTACAGGTTGTTGCAAGACATGAAGATTT ACTGGCACAAGCAACTGGGATTGAGTCGTTGGAAGGTGTTCTTCAGATGATGCAGACGAGAATTGGGGCT TTACAGGGAGCTGTTGATAGGATAAAAGCAAAAATTGTTGAACCATACAATAAGATAGTTGCCCGGACTG CACAACTAGCAAGACTTCAGGTTGCCTGTGATTTGCTTCGGAGGATTATTCGTATCTTGAATCTCAGTAA GAGACTCCAAGGACAACTGCAAGGGGGAAGTAGAGAGATAACAAAAGCTGCTCAGAGTCTCAATGAACTT GATTATCTTTCTCAAGGAATAGATCTTTCTGGAATAGAAGTGATAGAAAATGATCTACTTTTTATTGCAA GAGCCCGACTTGAAGTGGAAAATCAAGCTAAGCGCCTACTAGAGCAGGGTTTGGAGACTCAGAATCCAAC TCAAGTCGGAACAGCTCTTCAGGTTTTCTATAATCTTGGAACTTTGAAGGATACTATTACCAGTGTTGTG GATGGATATTGTGCTACTTTAGAAGAAAATATCAACAGTGCATTAGACATAAAAGTTTTGACTCAGCCTT CCCAGTCAGCTGTGAGAGGGGGACCTGGACGATCTACCATGCCAACCCCAGGAAATACTGCAGCTTTGCG TGCCTCATTCTGGACCAATATGGAGAAACTTATGGATCATATTTATGCTGTTTGTGGACAGGTACAACAT CTACAAAAAGTATTGGCCAAGAAGAGAGATCCTGTTTCTCACATTTGTTTCATTGAAGAAATAGTTAAGG ATGGACAACCGGAAATTTTCTACACATTTTGGAATTCAGTTACTCAGGCACTTTCTTCTCAATTTCATAT GGCAACAAACTCTTCGATGTTTTTGAAGCAGGCATTTGAAGGAGAATACCCTAAATTATTACGTCTTTAT AATGACTTATGGAAGCGTCTTCAACAATACAGTCAGCATATCCAAGGGAATTTTAATGCAAGTGGAACTA CAGACCTCTATGTTGACCTACAACACATGGAAGATGATGCACAAGATATATTCATACCAAAAAAGCCAGA TTATGATCCAGAAAAGGCTTTGAAAGACTCACTACAACCCTATGAGGCTGCTTATCTATCAAAATCCTTA TCTCGACTCTTCGATCCTATCAACTTGGTTTTTCCCCCGGGTGGTCGTAATCCTCCTTCCTCTGATGAAC TTGATGGTATTATTAAAACTATAGCAAGTGAACTAAATGTTGCTGCTGTTGATACAAACCTCACATTAGC TGTGTCAAAAAATGTGGCAAAGACCATCCAGTTATACAGTGTAAAATCAGAGCAGCTTCTCTCCACACAA GGAGATGCAAGTCAGGTGATTGGGCCTCTTACTGAAGGACAGAGAAGAAATGTGGCAGTAGTGAATTCAT TGTATAAGTTGCACCAATCAGTAACAAAGGCTATTCATGCTCTTATGGAAAATGCTGTGCAACCCTTACT CACTTCTGTGGGAGATGCTATAGAGGCCATAATCATCACCATGCATCAAGAAGACTTTTCTGGGTCATTA TCCAGCTCAGGAAAACCTGATGTTCCTTGTTCTCTGTACATGAAGGAGCTACAAGGTTTCATTGCCAGAG TTATGAGTGACTATTTTAAACACTTTGAATGCTTGGATTTTGTCTTTGACAACACTGAGGCTATTGCCCA AAGAGCTGTTGAACTTTTTATCCGCCATGCCAGTCTCATAAGACCTCTTGGTGAAGGTGGGAAAATGCGA CTTGCTGCTGATTTTGCACAGATGGAGTTGGCTGTGGGTCCATTCTGTAGACGAGTATCTGATTTAGGAA AGTCCTATCGGATGCTGAGATCATTCAGACCTCTGCTCTTCCAGGCAAGTGAACATGTAGCCAGTAGTCC TGCATTGGGGGATGTGATTCCGTTCAGCATCATTATTCAGTTTTTGTTCACGAGAGCACCCGCTGAACTG AAATCTCCTTTCCAGAGGGCAGAGTGGTCCCACACACGCTTCTCTCAGTGGCTGGATGACCATCCATCTG AAAAGGACAGGCTCCTCCTCATCAGGGGAGCCCTGGAAGCTTATGTTCAATCAGTGAGAAGTAGAGAAGG CAAAGAATTTGCACCAGTTTATCCCATAATGGTTCAGCTGCTTCAAAAGGCTATGTCTGCTCTTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220704 representing NM_181733
Red=Cloning site Green=Tags(s) MGWVGGRRRDSASPPGRSRSAADDINPAPANMEGGGGSVAVAGLGARGSGAAAATVRELLQDGCYSDFLN EDFDVKTYTSQSIHQAVIAEQLAKLAQGISQLDRELHLQVVARHEDLLAQATGIESLEGVLQMMQTRIGA LQGAVDRIKAKIVEPYNKIVARTAQLARLQVACDLLRRIIRILNLSKRLQGQLQGGSREITKAAQSLNEL DYLSQGIDLSGIEVIENDLLFIARARLEVENQAKRLLEQGLETQNPTQVGTALQVFYNLGTLKDTITSVV DGYCATLEENINSALDIKVLTQPSQSAVRGGPGRSTMPTPGNTAALRASFWTNMEKLMDHIYAVCGQVQH LQKVLAKKRDPVSHICFIEEIVKDGQPEIFYTFWNSVTQALSSQFHMATNSSMFLKQAFEGEYPKLLRLY NDLWKRLQQYSQHIQGNFNASGTTDLYVDLQHMEDDAQDIFIPKKPDYDPEKALKDSLQPYEAAYLSKSL SRLFDPINLVFPPGGRNPPSSDELDGIIKTIASELNVAAVDTNLTLAVSKNVAKTIQLYSVKSEQLLSTQ GDASQVIGPLTEGQRRNVAVVNSLYKLHQSVTKAIHALMENAVQPLLTSVGDAIEAIIITMHQEDFSGSL SSSGKPDVPCSLYMKELQGFIARVMSDYFKHFECLDFVFDNTEAIAQRAVELFIRHASLIRPLGEGGKMR LAADFAQMELAVGPFCRRVSDLGKSYRMLRSFRPLLFQASEHVASSPALGDVIPFSIIIQFLFTRAPAEL KSPFQRAEWSHTRFSQWLDDHPSEKDRLLLIRGALEAYVQSVRSREGKEFAPVYPIMVQLLQKAMSALQ TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_181733 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181733.2, NP_859422.2 |
| RefSeq Size | 4817 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 10466 |
| UniProt ID | Q9UP83 |
| Gene Summary | The protein encoded by this gene is one of eight proteins (Cog1-8) which form a Golgi-localized complex (COG) required for normal Golgi morphology and function. The encoded protein is organized with conserved oligomeric Golgi complex components 6, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants. Mutations in this gene result in congenital disorder of glycosylation type 2I.[provided by RefSeq, Jan 2011] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220704 | COG5 (Myc-DDK-tagged)-Human component of oligomeric golgi complex 5 (COG5), transcript variant 2 |
CNY 8840.00 |
|
| RC220704L3 | Lenti-ORF clone of COG5 (Myc-DDK-tagged)-Human component of oligomeric golgi complex 5 (COG5), transcript variant 2 |
CNY 10740.00 |
|
| RC220704L4 | Lenti-ORF clone of COG5 (mGFP-tagged)-Human component of oligomeric golgi complex 5 (COG5), transcript variant 2 |
CNY 10740.00 |
|
| SC309612 | COG5 (untagged)-Human component of oligomeric golgi complex 5 (COG5), transcript variant 2 |
CNY 16250.00 |
