United States
Germany
Japan
United Kingdom
China
PDE5A (NM_033430) Human Tagged ORF Clone
CAT＃: RG218974
- TrueORF®
PDE5A (tGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2
ORF Plasmid: DDK
"NM_033430" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,704.00
CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CGB-PDE; CN5A; PDE5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218974 representing NM_033430
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCCCTTTGGAGACAAAACAAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCC CTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGC AGATAACAGTGCCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCC ATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTC TAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATAT TTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCT GACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTG ATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCAT TGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAAT GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGG AAGAGGTTGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGA TGAAAAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACT TCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAAT CATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCAT TTTCATAGTGGATGAAGATTGCTCCGATTCTTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAA AAATCATCTGATACATTAACAAGGGAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA AAAATACTATGGAACCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAA TACAGGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAAT AAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACC GAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTA TGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCA GCAGAGGAAGAAACAAGAGAGCTACAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAA TTACTGACTTTAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTT TACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTT AAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGT TTGCTGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGC TGCACTAAGCCACGATTTGGATCACCGTGGTGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTT GCCCAGCTTTACTGCCATTCAATCATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTC CAGGCAATCAGATTCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGC TATTTTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAAT CAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTT CTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCA AGGAGACAGAGAGAGAAAAGAACTCAACATAGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAA ATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGT CAGAGGACTGTTTCCCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACA GCAGGAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218974 representing NM_033430
Red=Cloning site Green=Tags(s) MLPFGDKTREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRP IVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISA DRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFN AEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYET SLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELE KSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKN KVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASA AEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSV KKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPL AQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKN QFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNK IPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033430 |
| ORF Size | 2499 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033430.3 |
| RefSeq Size | 6836 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 8654 |
| UniProt ID | O76074 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218974 | PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2 |
CNY 6,104.00 |
|
| RC218974L1 | Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, Myc-DDK-tagged |
CNY 8,504.00 |
|
| RC218974L2 | Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, mGFP tagged |
CNY 10,640.00 |
|
| RC218974L3 | Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, Myc-DDK-tagged |
CNY 10,640.00 |
|
| RC218974L4 | Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, mGFP tagged |
CNY 10,640.00 |
|
| SC120663 | PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2 |
CNY 6,112.00 |
