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DROSHA (NM_013235) Human Tagged ORF Clone
CAT＃: RG213916
- TrueORF®
DROSHA (tGFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 1
ORF Plasmid: DDK
"NM_013235" in other vectors (6)
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CNY 15,010.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213916 representing NM_013235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGGAGTTACGGTTTATCGGTTGTTCCTGAACCTGC TGGATGCACACCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCCCCACCCCTGGAGATT GTGAATCATCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTG ACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCC TGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCATTCAGAAAAC TACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGAACGAGTAGGCTTCGTGACT TATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAAAAGGCCAAAGCTGCTCGGCCTCCGTGGGA ACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGAC AGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCC ACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAA ATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCCATCAAGCCC TGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACAGTCTCCCCGCCTACGAACT TTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCACGAGTATATCTTTGAAGGATTTTCTATGTT TGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAATTAGATTCAACATAGACTACACGATTCAT TTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCA GAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCC AAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTGTCCATGCAC CAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAGGAGATTGCCAATATGCTTC AGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGCAAAGGCATGATTGTTACCAACCCTGGGAC GAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCG ATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGT GGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAA GCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAAGTAACGGTG GAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGTCAGCATGCAATGATGCTAC CTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATGCATTTGGACAAGTTGATAGGATATACTTT CCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCT GATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATC ACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCC AACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAATTTCTGACC AGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCAACCTATCGGACTGCCATTG TTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAACTGGATCCATTTATGCTGTATGCTCACGG GCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCT GTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACC TGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACT TATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATTTTTACTCAT GTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCATCTGACCCTAGGCCACAATC AGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTAGCCACAGAGTACTTATTCATTCATTTCCC AGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTA GCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCA CCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACACTGATAAGGATTTGGAATATGT TCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAATTCATTTTGAATCAGGATTGGAATGAC CCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAAGAGCCAGACATTCCTCTGT ACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACCTACACTGTGGCTGTTTATTTCAAGGGAGA AAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAA AAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAG AAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213916 representing NM_013235
Red=Cloning site Green=Tags(s) MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPN FLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQG TFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPY PLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRS YERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEI VNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSEN YYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDED STCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKP CRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIH FIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMH QILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFP IIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTV ELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNP DHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLT SVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDPFMLYAHGPDLCRESDLRHAMANCFEALIGA VYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTH VRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKV AEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYTDKDLEYVHTFMNVCFFPRLKEFILNQDWND PKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALE KYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013235 |
| ORF Size | 4122 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013235.3 |
| RefSeq Size | 4764 bp |
| RefSeq ORF | 4125 bp |
| Locus ID | 29102 |
| UniProt ID | Q9NRR4 |
| Domains | RIBOc, DSRM |
| Gene Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213916 | DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
CNY 14,104.00 |
|
| RC213916L1 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
CNY 15,580.00 |
|
| RC213916L2 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
CNY 15,580.00 |
|
| RC213916L3 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
CNY 15,580.00 |
|
| RC213916L4 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
CNY 15,580.00 |
|
| SC308939 | DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
CNY 26,510.00 |
