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RNF22 (TRIM3) (NM_006458) Human Tagged ORF Clone
CAT＃: RG211739
- TrueORF®
TRIM3 (tGFP-tagged) - Human tripartite motif containing 3 (TRIM3), transcript variant 1
ORF Plasmid: DDK
"NM_006458" in other vectors (6)
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CNY 4,370.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BERP; HAC1; RNF22; RNF97 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211739 representing NM_006458
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAGGGAGGACAGCCCTGGCCCAGAGGTCCAGCCAATGGACAAGCAGTTCCTGGTATGCAGCA TCTGCCTGGATCGGTACCAGTGCCCCAAGGTTCTTCCTTGCCTGCACACCTTCTGTGAGAGATGTCTCCA AAACTATATCCCTGCCCAGAGCCTGACGCTATCCTGTCCAGTATGCCGGCAGACGTCCATCCTCCCAGAG CAGGGCGTCTCGGCACTGCAGAACAACTTCTTCATCAGCAGCCTCATGGAGGCAATGCAGCAGGCACCTG ATGGGGCCCACGACCCGGAGGACCCCCACCCCCTCAGTGTAGTGGCTGGCCGCCCTCTCTCCTGCCCCAA CCATGAAGGCAAGACGATGGAGTTTTACTGTGAGGCCTGTGAGACGGCCATGTGTGGTGAGTGCCGCGCC GGGGAGCATCGTGAGCATGGCACAGTGCTGCTGAGGGATGTGGTGGAGCAGCACAAGGCGGCCCTGCAGC GCCAGCTCGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCCGCAGCAATTGCCTTAGTCGGGGGCATCAG CCAGCAGCTGCAGGAGCGCAAGGCAGAGGCCCTGGCCCAGATTAGTGCAGCGTTCGAGGACCTGGAGCAA GCACTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACCTGGAGACCATTTGTGGGGCCAAACAGAAGGTGT TGCAAAGCCAGCTGGACACACTGCGCCAGGGTCAGGAACACATCGGCAGTAGCTGCAGCTTTGCAGAGCA GGCACTGCGCCTGGGCTCGGCCCCGGAGGTGTTGCTGGTGCGCAAGCACATGCGAGAGCGGCTGGCTGCA TTGGCGGCACAGGCCTTCCCGGAGCGGCCACATGAGAATGCACAGCTGGAACTGGTCCTTGAGGTGGACG GTCTGCGGCGATCGGTGCTCAATCTGGGCGCACTGCTCACCACGAGCGCCACTGCACACGAAACGGTGGC CACGGGAGAGGGCCTGCGCCAGGCGCTAGTGGGCCAGCCTGCCTCGCTCACTGTCACTACCAAAGACAAG GACGGGCGGTTGGTGCGCACAGGCAGCGCTGAGCTGCGTGCAGAGATCACCGGCCCGGACGGCACGCGCC TTCCAGTGCCAGTGGTGGACCACAAGAATGGCACATATGAGCTAGTGTACACAGCGCGCACGGAAGGCGA GCTGCTCCTCTCGGTGCTGCTCTACGGACAGCCAGTGCGCGGCAGCCCCTTCCGCGTGCGTGCCCTGCGT CCGGGGGACCTGCCACCTTCCCCGGACGATGTGAAGCGCCGTGTCAAGTCCCCTGGCGGCCCCGGCAGCC ATGTGCGCCAGAAGGCAGTGCGTAGGCCCAGCTCCATGTACAGCACAGGCGGCAAACGAAAGGACAACCC AATTGAGGATGAGCTCGTCTTCCGTGTTGGCAGTCGTGGAAGGGAGAAAGGTGAATTCACCAATTTACAA GGTGTGTCCGCAGCCAGCAGCGGCCGCATCGTGGTAGCAGACAGCAACAACCAGTGTATTCAGGTTTTCT CCAATGAGGGCCAGTTCAAGTTCCGTTTTGGGGTCCGAGGACGCTCACCTGGGCAGCTGCAGCGCCCCAC AGGTGTGGCAGTGGACACCAATGGAGACATAATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTC TCCCCTGAGGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGCCCCAAGGGAGTGGCCGTAG ACCGGAATGGACATATCATTGTGGTCGACAACAAGTCTTGCTGCGTCTTTACCTTCCAGCCCAATGGCAA ACTGGTTGGCCGTTTTGGGGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCCCATTTTGTGGCTGTG AACAACAAGAATGAAATTGTAGTAACGGACTTCCATAACCATTCAGTGAAGGTGTACAGTGCCGATGGAG AGTTCCTCTTCAAGTTTGGCTCCCATGGCGAGGGCAATGGGCAGTTCAATGCCCCCACAGGAGTAGCTGT GGACTCCAATGGAAACATCATTGTGGCTGACTGGGGCAACAGCCGCATCCAGGTATTCGACAGCTCTGGC TCCTTCCTGTCCTATATCAACACATCTGCAGAACCACTGTATGGTCCACAGGGCCTGGCACTGACCTCGG ATGGCCATGTGGTGGTGGCTGATGCTGGCAACCACTGCTTTAAAGCCTATCGCTACCTCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211739 representing NM_006458
Red=Cloning site Green=Tags(s) MAKREDSPGPEVQPMDKQFLVCSICLDRYQCPKVLPCLHTFCERCLQNYIPAQSLTLSCPVCRQTSILPE QGVSALQNNFFISSLMEAMQQAPDGAHDPEDPHPLSVVAGRPLSCPNHEGKTMEFYCEACETAMCGECRA GEHREHGTVLLRDVVEQHKAALQRQLEAVRGRLPQLSAAIALVGGISQQLQERKAEALAQISAAFEDLEQ ALQQRKQALVSDLETICGAKQKVLQSQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAA LAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHETVATGEGLRQALVGQPASLTVTTKDK DGRLVRTGSAELRAEITGPDGTRLPVPVVDHKNGTYELVYTARTEGELLLSVLLYGQPVRGSPFRVRALR PGDLPPSPDDVKRRVKSPGGPGSHVRQKAVRRPSSMYSTGGKRKDNPIEDELVFRVGSRGREKGEFTNLQ GVSAASSGRIVVADSNNQCIQVFSNEGQFKFRFGVRGRSPGQLQRPTGVAVDTNGDIIVADYDNRWVSIF SPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKSCCVFTFQPNGKLVGRFGGRGATDRHFAGPHFVAV NNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDSSG SFLSYINTSAEPLYGPQGLALTSDGHVVVADAGNHCFKAYRYLQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006458 |
| ORF Size | 2232 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006458.2, NP_006449.2 |
| RefSeq Size | 3059 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 10612 |
| UniProt ID | O75382 |
| Domains | zf-B_box, NHL, Filamin, RING, BBC |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Drosophila Brat and human ortholog TRIM3 maintain stem cell equilibrium and suppress brain tumorigenesis by attenuating Notch nuclear transport
,null,
Cancer research
,PubMed ID 26893479
[TRIM3]
|
|
Human Brat ortholog TRIM3 is a tumor suppressor that regulates asymmetric cell division in glioblastoma
,Chen, G;Kong, J;Tucker-Burden, C;Anand, M;Rong, Y;Rahman, F;Moreno, CS;Van Meir, EG;Hadjipanayis, CG;Brat, DJ;,
Cancer Res.
,PubMed ID 24947043
[TRIM3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211739 | TRIM3 (Myc-DDK-tagged)-Human tripartite motif containing 3 (TRIM3), transcript variant 1 |
CNY 3,990.00 |
|
| RC211739L1 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211739L2 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC211739L3 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211739L4 | Lenti ORF clone of Human tripartite motif containing 3 (TRIM3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC110176 | TRIM3 (untagged)-Human tripartite motif containing 3 (TRIM3), transcript variant 1 |
CNY 5,456.00 |
