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PODN (NM_153703) Human Tagged ORF Clone
CAT＃: RG205567
- TrueORF®
PODN (tGFP-tagged) - Human podocan (PODN), transcript variant 1
ORF Plasmid: DDK
"NM_153703" in other vectors (5)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PCAN; SLRR5A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205567 representing NM_153703
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGAGCCCGAGCCCGCGGAGCGCAGCTGAGACTGGGGGAGCGCGTTCGGCCTGTGGGGCGCCGCT CGGCGCCGGGGCGCAGCAGGTTCCATCAGCCCTGGCGCCCAGGCGCATCTGACTCGGCACCCCCTGCAGG CACCATGGCCCAGAGCCGGGTGCTGCTGCTCCTGCTGCTGCTGCCGCCACAGCTGCACCTGGGACCTGTG CTTGCCGTGAGGGCCCCAGGATTTGGCCGAAGTGGCGGCCACAGCCTGAGCCCCGAAGAGAACGAATTTG CGGAGGAGGAGCCGGTGCTGGTACTGAGCCCTGAGGAGCCCGGGCCTGGCCCAGCCGCGGTCAGCTGCCC CCGAGACTGTGCCTGTTCCCAGGAGGGCGTCGTGGACTGTGGCGGTATTGACCTGCGTGAGTTCCCGGGG GACCTGCCTGAGCACACCAACCACCTATCTCTGCAGAACAACCAGCTGGAAAAGATCTACCCTGAGGAGC TCTCCCGGCTGCACCGGCTGGAGACACTGAACCTGCAAAACAACCGCCTGACTTCCCGAGGGCTCCCAGA GAAGGCGTTTGAGCATCTGACCAACCTCAATTACCTGTACTTGGCCAATAACAAGCTGACCTTGGCACCC CGCTTCCTGCCAAACGCCCTGATCAGTGTGGACTTTGCTGCCAACTATCTCACCAAGATCTATGGGCTCA CCTTTGGCCAGAAGCCAAACTTGAGGTCTGTGTACCTGCACAACAACAAGCTGGCAGACGCCGGGCTGCC GGACAACATGTTCAACGGCTCCAGCAACGTCGAGGTCCTCATCCTGTCCAGCAACTTCCTGCGCCACGTG CCCAAGCACCTGCCGCCTGCCCTGTACAAGCTGCACCTCAAGAACAACAAGCTGGAGAAGATCCCCCCGG GGGCCTTCAGCGAGCTGAGCAGCCTGCGCGAGCTATACCTGCAGAACAACTACCTGACTGACGAGGGCCT GGACAACGAGACCTTCTGGAAGCTCTCCAGCCTGGAGTACCTGGATCTGTCCAGCAACAACCTGTCTCGG GTCCCAGCTGGGCTGCCGCGCAGCCTGGTGCTGCTGCACTTGGAGAAGAACGCCATCCGGAGCGTGGACG CGAATGTGCTGACCCCCATCCGCAGCCTGGAGTACCTGCTGCTGCACAGCAACCAGCTGCGGGAGCAGGG CATCCACCCACTGGCCTTCCAGGGCCTCAAGCGGTTGCACACGGTGCACCTGTACAACAACGCGCTGGAG CGCGTGCCCAGTGGCCTGCCTCGCCGCGTGCGCACCCTCATGATCCTGCACAACCAGATCACAGGCATTG GCCGCGAAGACTTTGCCACCACCTACTTCCTGGAGGAGCTCAACCTCAGCTACAACCGCATCACCAGCCC ACAGGTGCACCGCGACGCCTTCCGCAAGCTGCGCCTGCTGCGCTCGCTGGACCTGTCGGGCAACCGGCTG CACACGCTGCCACCTGGGCTGCCTCGAAATGTCCATGTGCTGAAGGTCAAGCGCAATGAGCTGGCTGCCT TGGCACGAGGGGCGCTGGCGGGCATGGCTCAGCTGCGTGAGCTGTACCTCACCAGCAACCGACTGCGCAG CCGAGCCCTGGGCCCCCGTGCCTGGGTGGACCTCGCCCATCTGCAGCTGCTGGACATCGCCGGGAATCAG CTCACAGAGATCCCCGAGGGGCTCCCCGAGTCACTTGAGTACCTGTACCTGCAGAACAACAAGATTAGTG CGGTGCCCGCCAATGCCTTCGACTCCACGCCCAACCTCAAGGGGATCTTTCTCAGGTTTAACAAGCTGGC TGTGGGCTCCGTGGTGGACAGTGCCTTCCGGAGGCTGAAGCACCTGCAGGTCTTGGACATTGAAGGCAAC TTAGAGTTTGGTGACATTTCCAAGGACCGTGGCCGCTTGGGGAAGGAAAAGGAGGAGGAGGAAGAGGAGG AGGAGGAGGAAGAGGAAACAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205567 representing NM_153703
Red=Cloning site Green=Tags(s) MEGARARGAQLRLGERVRPVGRRSAPGRSRFHQPWRPGASDSAPPAGTMAQSRVLLLLLLLPPQLHLGPV LAVRAPGFGRSGGHSLSPEENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPG DLPEHTNHLSLQNNQLEKIYPEELSRLHRLETLNLQNNRLTSRGLPEKAFEHLTNLNYLYLANNKLTLAP RFLPNALISVDFAANYLTKIYGLTFGQKPNLRSVYLHNNKLADAGLPDNMFNGSSNVEVLILSSNFLRHV PKHLPPALYKLHLKNNKLEKIPPGAFSELSSLRELYLQNNYLTDEGLDNETFWKLSSLEYLDLSSNNLSR VPAGLPRSLVLLHLEKNAIRSVDANVLTPIRSLEYLLLHSNQLREQGIHPLAFQGLKRLHTVHLYNNALE RVPSGLPRRVRTLMILHNQITGIGREDFATTYFLEELNLSYNRITSPQVHRDAFRKLRLLRSLDLSGNRL HTLPPGLPRNVHVLKVKRNELAALARGALAGMAQLRELYLTSNRLRSRALGPRAWVDLAHLQLLDIAGNQ LTEIPEGLPESLEYLYLQNNKISAVPANAFDSTPNLKGIFLRFNKLAVGSVVDSAFRRLKHLQVLDIEGN LEFGDISKDRGRLGKEKEEEEEEEEEEEETR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153703 |
| ORF Size | 1983 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153703.3, NP_714914.2 |
| RefSeq Size | 3019 bp |
| RefSeq ORF | 1842 bp |
| Locus ID | 127435 |
| UniProt ID | Q7Z5L7 |
| Domains | LRR, LRR_TYP, LRR_BAC, LRR_PS |
| Protein Families | Secreted Protein |
| Gene Summary | The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205567 | PODN (Myc-DDK-tagged)-Human podocan (PODN), transcript variant 1 |
CNY 4,920.00 |
|
| RC205567L3 | Lenti ORF clone of Human podocan (PODN), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC205567L4 | Lenti ORF clone of Human podocan (PODN), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| SC100964 | PODN (untagged)-Human podocan (PODN), transcript variant 1 |
CNY 7,384.00 |
|
| SC318121 | PODN (untagged)-Human podocan (PODN), transcript variant 1 |
CNY 10,550.00 |
