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GPRC5B (NM_016235) Human Tagged ORF Clone
CAT＃: RG205201
- TrueORF®
GPRC5B (tGFP-tagged) - Human G protein-coupled receptor, family C, group 5, member B (GPRC5B)
ORF Plasmid: DDK
"NM_016235" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RAIG-2; RAIG2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205201 representing NM_016235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGTGGCATCAGAGAGAAAGATGAGAGCTCACCAGGTGCTCACCTTCCTCCTGCTCTTCGTGATCA CCTCGGTGGCCTCTGAAAACGCCAGCACATCCCGAGGCTGTGGGCTGGACCTCCTCCCTCAGTACGTGTC CCTGTGCGACCTGGACGCCATCTGGGGCATTGTGGTGGAGGCGGTGGCCGGGGCGGGCGCCCTGATCACA CTGCTCCTGATGCTCATCCTCCTGGTGCGGCTGCCCTTCATCAAGGAGAAGGAGAAGAAGAGCCCTGTGG GCCTCCACTTTCTGTTCCTCCTGGGGACCCTGGGCCTCTTTGGGCTGACGTTTGCCTTCATCATCCAGGA GGACGAGACCATCTGCTCTGTCCGCCGCTTCCTCTGGGGCGTCCTCTTTGCGCTCTGCTTCTCCTGCCTG CTGAGCCAGGCATGGCGCGTGCGGAGGCTGGTGCGGCATGGCACGGGCCCCGCGGGCTGGCAGCTGGTGG GCCTGGCGCTGTGCCTGATGCTGGTGCAAGTCATCATCGCTGTGGAGTGGCTGGTGCTCACCGTGCTGCG TGACACAAGGCCAGCCTGCGCCTACGAGCCCATGGACTTTGTGATGGCCCTCATCTACGACATGGTACTG CTTGTGGTCACCCTGGGGCTGGCCCTCTTCACTCTGTGCGGCAAGTTCAAGAGGTGGAAGCTGAACGGGG CCTTCCTCCTCATCACAGCCTTCCTCTCTGTGCTCATCTGGGTGGCCTGGATGACCATGTACCTCTTCGG CAATGTCAAGCTGCAGCAGGGGGATGCCTGGAACGACCCCACCTTGGCCATCACGCTGGCGGCCAGCGGC TGGGTCTTCGTCATCTTCCACGCCATCCCTGAGATCCACTGCACCCTTCTGCCAGCCCTGCAGGAGAACA CGCCCAACTACTTCGACACGTCGCAGCCCAGGATGCGGGAGACGGCCTTCGAGGAGGACGTGCAGCTGCC GCGGGCCTATATGGAGAACAAGGCCTTCTCCATGGATGAACACAATGCAGCTCTCCGAACAGCAGGATTT CCCAACGGCAGCTTGGGAAAAAGACCCAGTGGCAGCTTGGGGAAAAGACCCAGCGCTCCGTTTAGAAGCA ACGTGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACCATCCCAACTGCTCCGCCAAGTCA CACAGGAAGACACCTTTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205201 representing NM_016235
Red=Cloning site Green=Tags(s) MFVASERKMRAHQVLTFLLLFVITSVASENASTSRGCGLDLLPQYVSLCDLDAIWGIVVEAVAGAGALIT LLLMLILLVRLPFIKEKEKKSPVGLHFLFLLGTLGLFGLTFAFIIQEDETICSVRRFLWGVLFALCFSCL LSQAWRVRRLVRHGTGPAGWQLVGLALCLMLVQVIIAVEWLVLTVLRDTRPACAYEPMDFVMALIYDMVL LVVTLGLALFTLCGKFKRWKLNGAFLLITAFLSVLIWVAWMTMYLFGNVKLQQGDAWNDPTLAITLAASG WVFVIFHAIPEIHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGF PNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAPPSHTGRHLW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016235 |
| ORF Size | 1209 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016235.3 |
| RefSeq Size | 2870 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 51704 |
| UniProt ID | Q9NZH0 |
| Domains | 7tm_3 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205201 | GPRC5B (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member B (GPRC5B) |
CNY 3,656.00 |
|
| RC205201L1 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member B (GPRC5B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205201L2 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member B (GPRC5B), mGFP tagged |
CNY 5,890.00 |
|
| RC205201L3 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member B (GPRC5B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205201L4 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member B (GPRC5B), mGFP tagged |
CNY 5,890.00 |
|
| SC114355 | GPRC5B (untagged)-Human G protein-coupled receptor, family C, group 5, member B (GPRC5B) |
CNY 2,950.00 |
