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Calpain 10 (CAPN10) (NM_023083) Human Tagged ORF Clone
CAT＃: RG202556
- TrueORF®
CAPN10 (tGFP-tagged) - Human calpain 10 (CAPN10), transcript variant 1
ORF Plasmid: DDK
"NM_023083" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CANP10; NIDDM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202556 representing NM_023083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCCGCCGCGGACT CCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGACATCACGTGGAGGCGGCCCCA GGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCACGGGAAGGGCAGGTGAAGCAGGGGCTGCTG GGGGATTGCTGGTTCCTGTGTGCCTGCGCCGCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTC CTCCGGGACAGCCGAGCTGGGCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGG ACGCTGGGTGGAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGGTCCTACGAGC ACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGCCTGGCAGAAAGATGGAACCT GAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCAGGCCGCTGGGAGCACAGGACTTGTCGGCAG CTGCTCCACCTGAAGGACCAGTGTCTGATCAGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGC TGGGGGAGTTCCATGCCTTCATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCT GCTGCTGCGGATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAGTTCTGGGTGG AGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCGGTCACGGAGGCCGGCCACCT GCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGGGCGCTGCCTGGGGCCTGGGTCAAGGGCCAG TCAGCAGGAGGCTGCCGGAACAACAGCGGCTTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAAC CGAGTGAGGTGTACATTGCCGTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCG GGCACTGGTGGGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATGCCCCCCGTGG CTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAGCTCTCACCGGGCTACTACCT GGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAGTTCCTGCTCCGAGTCTTCTCTACCGGGCGA GTCTCCCTTAGCGCCATCAGGGCAGTGGCCAAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGT GGGGGACCGTGCAGCTACGGGGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTC ATACCCCACCAACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTCCAGGTCCCAG AGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTGCTGAGCTGCGTGCCACATCG CTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCAGGCACCTACAAGGTTGTGCCCTCCACCTAC CTGCCGGACACAGAGGGGGCCTTCACAGTGACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCC AGGAGATGCTGGGCCAGTTCCTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202556 representing NM_023083
Red=Cloning site Green=Tags(s) MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLL GDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRC QREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQ LLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEG WSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQ SAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAV GLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGR VSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRIT LHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTY LPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023083 |
| ORF Size | 2016 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023083.1, NP_075571.1 |
| RefSeq Size | 2626 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 11132 |
| UniProt ID | Q9HC96 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202556 | CAPN10 (Myc-DDK-tagged)-Human calpain 10 (CAPN10), transcript variant 1 |
CNY 3,990.00 |
|
| RC202556L3 | Lenti ORF clone of Human calpain 10 (CAPN10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202556L4 | Lenti ORF clone of Human calpain 10 (CAPN10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC124129 | CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 1 |
CNY 2,950.00 |
|
| SC320709 | CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 1 |
CNY 2,950.00 |
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