PSMD2 (NM_001278708) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	P97; RPN1; S2; TRAP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239543 representing NM_001278708 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGAGTGGGGAGCGTGAGTGCCTCAAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCAT GGGGTCATGAGTATGTCAGGCATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGA GAAGGTCCAGCGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGACATTGATGAAA ATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTGCCTGAGCCTGAGAACTCAGC CCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGCCGCTTCCCTGAAGCTCTGAGATTGGCATTG ATGCTCAATGACATGGAGTTGGTAGAAGACATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGA TGGCATTCATGCTAGGCCGGCATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCT GACAGAGATCATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGCAGTGGCTCTC AGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTTGTGAATGCAGCTTTTGGCCA AGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTACAAGAACAAGGACCACGGAATGTTGAGTGCA GCTGCATCTCTTGGGATGATTCTGCTGTGGGATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGT ACTCCTCTGAGGACTACATTAAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAA TGAGTGTGACCCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTGCTGCTGCCTG TGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTAGCCTGTGGAATGATAGCAGT AGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAGACCATCATGGAGAAGTCAGAGACTGAGCTC AAGGATACTTATGCTCGTTGGCTTCCTCTTGGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCG AGGCAATCCTGGCTGCACTGGAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGT GTGTGCATATGCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAGGAAGCCCCTG CTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATTGCTATGGGGGAGGAGATTGG TGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGATATGGGGAGCCTACACTCCGGAGGGCTGTA CCTTTAGCACTGGCCCTCATCTCTGTTTCAAATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCT CTCATGATGCTGATCCAGAAGTTTCCTATAACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAA TAATGCCCGTCTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCCTACCACAGCG ACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCTCTTTCCTGGATGTTCG AAACATTATTCTAGGCAAATCACACTATGTATTGTATGGGCTGGTGGCTGCCATGCAGCCCCGAATGCTG GTTACGTTTGATGAGGAGCTGCGGCCATTGCCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGG GCCAGGCTGGCAAGCCGAAGACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGG GGAACGGGCAGAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT CGGAAGAACCCCAATTATGATCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239543 representing NM_001278708 Red=Cloning site Green=Tags(s) MTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNA EHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLAL MLNDMELVEDIFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIM EPKVPDDIYKTHLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSA AASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLG SIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETEL KDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHF DSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAV PLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLF MVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRML VTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVIL RKNPNYDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278708
ORF Size	2334 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278708.2
RefSeq Size	2655 bp
RefSeq ORF	2337 bp
Locus ID	5708
UniProt ID	Q13200
Protein Families	Druggable Genome
Protein Pathways	Proteasome
MW	86.1 kDa
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

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