CRMP1 (NM_001288661) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238931 representing NM_001288661 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAAATGAACAACGAAGTGGTGGACAAGAGTGACCGACTCCTCATCAAAGGTGGACGGATCATCA ACGATGACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGAACTT AATCGTTCCTGGTGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATTGATGTC AACACGTACCTGCAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGGGCGG CACTGGTGGGCGGGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTGACCTC TTTCGAGAAGTGGCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGACATCACA AGCTGGTACGATGGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCCTTCCAAG TCTACATGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACCTTCCTTAA GGGCCTGGGAGCTGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAGCGGATC CTGGAGATGGGCATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCGAGGCGG TGTTCCGGGCCATCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAGCAAGAG TGCAGCCGACATCATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCCGCCAGC CTGGGGACCGATGGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTCGTGACTTCCCCTC CCCTGAGCCCGGACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGGGACTTGCAGGTCAC AGGCAGCGGCCACTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAACTTTACCCTGATCCCC GAGGGTGTCAACGGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGCTACTGGCAAAATGG ATGAGAACCAGTTTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAGGAAAGG GCGGATTGCCGTGGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATAACAGCC AAAAGTCACAAGTCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCACGGCTCCCCACTAGTGG TCATCAGCCAGGGCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAACAAGGGCATGGGCCGCTTCAT TCCGCGGAAGGCGTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGGAATAAGGTTTTTGGATTGCAA GGGGTTTCCAGGGGCATGTATGACGGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAACTCCCG CTCCTTCAGCCAAATCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTCCAACTT CAGCTTATCAGGTGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCGCCCCCT GGTGGCCGCTCCAACATCACCAGCCTCGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238931 representing NM_001288661 Red=Cloning site Green=Tags(s) MQKMNNEVVDKSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDV NTYLQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDIT SWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRI LEMGITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAAS LGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIP EGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITA KSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQ GVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPP GGRSNITSLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288661
ORF Size	1710 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288661.2
RefSeq Size	3278 bp
RefSeq ORF	1713 bp
Locus ID	1400
UniProt ID	Q14194
MW	62.5 kDa
Gene Summary	This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

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