CRMP1 (NM_001288662) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238917 representing NM_001288662 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGACCACTGGAGCCCATAGTGACCGACTCCTCATCAAAGGTGGACGGATCATCAACGATGACCAAT CCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGAACTTAATCGTTCCTGG TGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATTGATGTCAACACGTACCTG CAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGGGCGGCACTGGTGGGCG GGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTGACCTCTTTCGAGAAGTG GCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGACATCACAAGCTGGTACGAT GGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCCTTCCAAGTCTACATGGCCT ATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACCTTCCTTAAGGGCCTGGGAGC TGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAGCGGATCCTGGAGATGGGC ATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCGAGGCGGTGTTCCGGGCCA TCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAGCAAGAGTGCAGCCGACAT CATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCCGCCAGCCTGGGGACCGAT GGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTCGTGACTTCCCCTCCCCTGAGCCCGG ACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGGGACTTGCAGGTCACAGGCAGCGGCCA CTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAACTTTACCCTGATCCCCGAGGGTGTCAAC GGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGCTACTGGCAAAATGGATGAGAACCAGT TTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAGGAAAGGGCGGATTGCCGT GGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATAACAGCCAAAAGTCACAAG TCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCACGGCTCCCCACTAGTGGTCATCAGCCAGG GCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAACAAGGGCATGGGCCGCTTCATTCCGCGGAAGGC GTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGGAATAAGGTTTTTGGATTGCAAGGGGTTTCCAGG GGCATGTATGACGGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAACTCCCGCTCCTTCAGCCA AATCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTCCAACTTCAGCTTATCAGG TGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCGCCCCCTGGTGGCCGCTCC AACATCACCAGCCTCGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238917 representing NM_001288662 Red=Cloning site Green=Tags(s) MRTTGAHSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYL QKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYD GVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMG ITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTD GTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVN GIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHK SAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSR GMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRS NITSLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288662
ORF Size	1698 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288662.1, NP_001275591.1
RefSeq Size	3101 bp
RefSeq ORF	1701 bp
Locus ID	1400
UniProt ID	Q14194
MW	61.9 kDa
Gene Summary	This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.