RNF22 (TRIM3) (NM_001248007) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BERP; HAC1; RNF22; RNF97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234595 representing NM_001248007 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACGATGGAGTTTTACTGTGAGGCCTGTGAGACGGCCATGTGTGGTGAGTGCCGCGCCGGGGAGC ATCGTGAGCATGGCACAGTGCTGCTGAGGGATGTGGTGGAGCAGCACAAGGCGGCCCTGCAGCGCCAGCT CGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCCGCAGCAATTGCCTTAGTCGGGGGCATCAGCCAGCAG CTGCAGGAGCGCAAGGCAGAGGCCCTGGCCCAGATCAGTGCAGCGTTCGAGGACCTGGAGCAAGCACTGC AGCAGCGCAAGCAGGCTCTGGTCAGCGACCTGGAGACCATTTGTGGGGCCAAACAGAAGGTGTTGCAAAG CCAGCTGGACACACTGCGCCAGGGTCAGGAACACATCGGCAGTAGCTGCAGCTTTGCAGAGCAGGCACTG CGCCTGGGCTCGGCCCCGGAGGTGTTGCTGGTGCGCAAGCACATGCGAGAGCGGCTGGCTGCATTGGCGG CACAGGCCTTCCCGGAGCGGCCACATGAGAATGCACAGCTGGAACTGGTCCTTGAGGTGGACGGTCTGCG GCGATCGGTGCTCAATCTGGGCGCACTGCTCACCACGAGCGCCACTGCACACGAAACGGTGGCCACGGGA GAGGGCCTGCGCCAGGCGCTAGTGGGCCAGCCTGCCTCGCTCACTGTCACTACCAAAGACAAGGACGGGC GGTTGGTGCGCACAGGCAGCGCTGAGCTGCGTGCAGAGATCACCGGCCCGGACGGCACGCGCCTTCCGGT GCCAGTGGTGGACCACAAGAATGGCACATATGAGCTAGTGTACACAGCGCGCACGGAAGGCGAGCTGCTC CTCTCGGTGCTGCTCTACGGACAGCCAGTGCGCGGCAGCCCCTTCCGCGTGCGTGCCCTGCGTCCGGGGG ACCTGCCACCTTCCCCGGACGATGTGAAGCGCCGTGTCAAGTCCCCTGGCGGCCCCGGCAGCCATGTGCG CCAGAAGGCAGTGCGTAGGCCCAGCTCCATGTACAGCACAGGCGGCAAACGAAAGGACAACCCAATTGAG GATGAGCTCGTCTTCCGTGTTGGCAGTCGTGGAAGGGAGAAAGGTGAATTCACCAATTTACAAGGTGTGT CCGCAGCCAGCAGCGGCCGCATCGTGGTAGCAGACAGCAACAACCAGTGTATTCAGGTTTTCTCCAATGA GGGCCAGTTCAAGTTCCGTTTTGGGGTCCGAGGACGCTCACCTGGGCAGCTGCAGCGCCCCACAGGTGTG GCAGTGGACACCAATGGAGACATAATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTCTCCCCTG AGGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGCCCCAAGGGAGTGGCCGTAGACCGGAA TGGACATATCATTGTGGTCGACAACAAGTCTTGCTGCGTCTTTACCTTCCAGCCCAATGGCAAACTGGTT GGCCGTTTTGGGGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCCCATTTTGTGGCTGTGAACAACA AGAATGAAATTGTAGTAACGGACTTCCATAACCATTCAGTGAAGGTGTACAGTGCCGATGGAGAGTTCCT CTTCAAGTTTGGCTCCCATGGCGAGGGCAATGGGCAGTTCAATGCCCCCACAGGAGTAGCTGTGGACTCC AATGGAAACATCATTGTGGCTGACTGGGGCAACAGCCGCATCCAGGTATTCGACAGCTCTGGCTCCTTCC TGTCCTATATCAACACATCTGCAGAACCACTGTATGGTCCACAGGGCCTGGCACTGACCTCGGATGGCCA TGTGGTGGTGGCTGATGCTGGCAACCACTGCTTTAAAGCCTATCGCTACCTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234595 representing NM_001248007 Red=Cloning site Green=Tags(s) MKTMEFYCEACETAMCGECRAGEHREHGTVLLRDVVEQHKAALQRQLEAVRGRLPQLSAAIALVGGISQQ LQERKAEALAQISAAFEDLEQALQQRKQALVSDLETICGAKQKVLQSQLDTLRQGQEHIGSSCSFAEQAL RLGSAPEVLLVRKHMRERLAALAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHETVATG EGLRQALVGQPASLTVTTKDKDGRLVRTGSAELRAEITGPDGTRLPVPVVDHKNGTYELVYTARTEGELL LSVLLYGQPVRGSPFRVRALRPGDLPPSPDDVKRRVKSPGGPGSHVRQKAVRRPSSMYSTGGKRKDNPIE DELVFRVGSRGREKGEFTNLQGVSAASSGRIVVADSNNQCIQVFSNEGQFKFRFGVRGRSPGQLQRPTGV AVDTNGDIIVADYDNRWVSIFSPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKSCCVFTFQPNGKLV GRFGGRGATDRHFAGPHFVAVNNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDS NGNIIVADWGNSRIQVFDSSGSFLSYINTSAEPLYGPQGLALTSDGHVVVADAGNHCFKAYRYLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001248007
ORF Size	1875 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001248007.1, NP_001234936.1
RefSeq Size	2730 bp
RefSeq ORF	1878 bp
Locus ID	10612
UniProt ID	O75382
MW	68.2 kDa
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.