MYO5A (NM_001142495) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GS1; MYH12; MYO5; MYR12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227187 representing NM_001142495 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGTCGGAGCTCTACACAAAGTTTGCCAGGGTTTGGATACCTGATCCAGAGGAAGTCTGGAAGT CAGCAGAGCTGCTCAAAGATTATAAGCCAGGAGATAAAGTCCTCCTGCTTCACCTCGAGGAAGGAAAGGA TTTGGAATACCATCTAGATCCAAAGACCAAGGAGCTGCCTCACTTACGAAATCCTGACATACTTGTTGGT GAAAATGACCTCACAGCCCTCAGCTATCTTCATGAGCCTGCTGTGCTCCATAATCTCAGAGTCCGCTTTA TTGATTCCAAACTTATTTATACGTATTGTGGTATAGTCCTAGTAGCTATAAATCCCTATGAACAGCTGCC TATTTATGGAGAAGATATTATTAATGCATACAGTGGTCAGAACATGGGTGATATGGATCCACATATCTTT GCAGTAGCTGAAGAAGCTTACAAGCAAATGGCCAGAGATGAACGAAATCAGTCCATCATCGTAAGTGGAG AGTCTGGGGCAGGAAAAACAGTCTCAGCTAAGTATGCCATGCGATACTTTGCAACTGTGAGTGGTTCTGC CAGTGAGGCCAATGTGGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCCATTGGAAATGCTAAA ACAACCAGGAATGATAATAGCAGCCGTTTTGGGAAGTATATTGAGATTGGTTTTGATAAGAGATATCGAA TCATTGGTGCCAATATGAGAACTTATCTTTTAGAGAAATCCAGAGTGGTATTCCAGGCAGAAGAGGAGAG AAACTATCATATCTTCTATCAGCTTTGTGCCTCAGCAAAGTTACCTGAATTTAAAATGCTACGATTAGGA AATGCAGATAACTTTAATTACACAAAACAAGGAGGCAGTCCTGTGATTGAAGGAGTGGATGATGCAAAGG AGATGGCACATACTAGGCAGGCCTGCACTTTGCTAGGAATTAGTGAATCTCATCAAATGGGAATTTTCCG AATACTTGCTGGCATCCTTCACTTAGGCAATGTTGGATTTACATCCCGAGATGCAGACAGCTGCACAATA CCTCCCAAGCATGAACCTCTCTGCATCTTCTGTGACCTCATGGGTGTGGACTATGAGGAGATGTGTCACT GGCTCTGCCATCGGAAACTGGCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCAC GAATGCCCGCGATGCTTTGGCCAAGCACATCTATGCCAAGCTCTTTAACTGGATTGTAGATAATGTCAAT CAGGCTCTCCATTCTGCTGTCAAACAGCACTCTTTTATTGGTGTGCTAGACATTTACGGATTTGAAACAT TTGAGATAAATAGTTTTGAACAGTTTTGCATAAATTATGCAAATGAAAAACTACAGCAACAATTCAATAT GCATGTCTTCAAATTGGAGCAAGAAGAATATATGAAGGAACAAATTCCATGGACACTCATAGATTTTTAT GATAATCAGCCTTGTATTAATCTTATAGAATCAAAACTAGGCATTCTAGATTTACTGGATGAGGAATGCA AGATGCCTAAAGGCACAGATGACACCTGGGCCCAAAAATTGTACAACACACATTTGAACAAATGTGCACT CTTTGAAAAGCCTCGTCTATCAAACAAAGCTTTCATCATCCAACATTTTGCTGACAAAGTGGAATACCAG TGTGAAGGATTTCTCGAAAAGAATAAAGACACCGTTTTTGAAGAACAAATTAAAGTTCTTAAATCAAGCA AGTTTAAGATGCTACCAGAACTATTTCAAGATGATGAGAAGGCCATCAGTCCAACTTCAGCCACCTCCTC AGGGCGCACACCCCTCACACGAACTCCTGCAAAGCCCACCAAAGGCAGACCAGGCCAAATGGCCAAAGAG CACAAGAAAACAGTGGGGCATCAGTTCAGAAACTCCCTGCACCTGCTTATGGAGACACTCAATGCCACTA CCCCTCACTATGTGCGCTGTATCAAGCCTAATGACTTCAAGTTCCCATTCACGTTTGATGAGAAGAGGGC AGTGCAGCAGCTGAGAGCATGTGGTGTCCTGGAAACCATCCGAATCAGTGCGGCCGGTTTCCCCTCACGG TGGACTTACCAAGAATTTTTCAGCCGCTACCGTGTCCTAATGAAGCAGAAAGATGTGCTGAGTGACAGAA AGCAAACATGCAAGAATGTGTTAGAGAAACTGATACTGGACAAGGACAAATACCAGTTTGGTAAGACAAA GATCTTTTTCCGTGCCGGTCAAGTGGCCTATCTAGAAAAATTGAGAGCTGACAAACTGAGAGCTGCCTGC ATCCGGATCCAGAAGACCATCCGAGGGTGGCTGCTGAGAAAGAAGTACCTACGCATGCGGAAGGCAGCCA TCACCATGCAGAGATACGTGCGGGGCTACCAGGCCCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC AGCAACCATCATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC ACTATTGTTCTTCAGTCTTACTTGCGAGGCTTCTTGGCCAGAAATAGGTATCGCAAGATACTCCGTGAGC ACAAAGCAGTCATCATTCAGAAGCGAGTCCGGGGCTGGCTGGCCCGCACACACTACAAGAGGAGCATGCA TGCCATCATCTACCTTCAGTGCTGCTTCAGGCGGATGATGGCCAAGCGTGAGCTAAAGAAGCTCAAAATC GAGGCTCGCTCAGTGGAGCGCTATAAGAAGCTGCACATCGGCATGGAGAACAAGATCATGCAGCTGCAGC GCAAAGTTGATGAGCAGAACAAAGACTACAAATGCCTTGTGGAGAAACTAACCAATCTGGAAGGAATATA CAACTCTGAGACTGAGAAACTACGAAGTGACTTAGAACGTCTTCAACTAAGTGAAGAGGAAGCGAAAGTT GCCACTGGGCGGGTCCTTAGTCTGCAGGAAGAAATTGCCAAGCTCCGGAAAGACCTGGAGCAAACTCGTT CAGAGAAAAAATGCATTGAGGAACATGCAGATCGATACAAACAAGAAACAGAGCAGCTGGTATCAAATCT GAAGGAAGAAAATACTTTGCTGAAGCAAGAAAAAGAAGCCCTCAATCACCGCATCGTGCAGCAGGCTAAG GAGATGACAGAAACTATGGAGAAGAAGTTAGTAGAAGAAACGAAACAACTGGAACTCGACCTTAATGATG AAAGGCTGAGATATCAGAACCTTCTGAATGAGTTCAGTCGCCTGGAAGAAAGATATGATGACCTCAAGGA AGAGATGACCCTTATGGTGCATGTGCCTAAGCCTGGACACAAGAGAACAGACTCCACCCACAGCAGCAAC GAGTCTGAATATATCTTTAGCTCTGAAATTGCAGAAATGGAAGACATTCCATCAAGGACAGAGGAACCAA GTGAGAAGAAGGTACCTCTGGACATGTCATTGTTCCTTAAGCTCCAGAAGCGGGTCACAGAGCTGGAGCA GGAGAAGCAGGTGATGCAGGATGAGCTGGACCGCAAGGAGGAGCAGGTGCTCCGCAGCAAGGCCAAGGAA GAAGAAAGACCACAAATTAGAGGTGCAGAACTGGAATATGAGTCACTCAAGCGTCAAGAACTAGAATCAG AAAACAAAAAACTGAAGAATGAGCTAAATGAGTTGCGCAAGGCCCTCAGTGAGAAAAGTGCCCCAGAGGT GACCGCCCCAGGTGCACCTGCCTACCGTGTCCTCATGGAGCAGCTGACCTCTGTGAGCGAGGAGCTTGAT GTCCGCAAGGAGGAAGTCCTCATCTTAAGGTCTCAACTGGTGAGCCAGAAAGAGGCCATCCAACCCAAGG ATGACAAGAATACAATGACAGATTCCACAATACTTTTGGAAGATGTACAAAAAATGAAAGATAAAGGTGA AATAGCACAAGCATACATTGGTTTGAAAGAAACAAATAGGCTCCTGGAATCCCAGCTGCAGTCACAGAAG AGGAGCCATGAGAATGAGGCCGAGGCCCTCCGTGGGGAGATCCAGAGCCTGAAGGAGGAGAACAACCGAC AGCAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCAGAGGCCCGCATTGAGGCCAGCCTGCAGCACGA GATCACCCGGCTGACCAACGAAAACTTGGATTTGATGGAACAACTTGAAAAACAGGATAAGACGGTCCGT AAACTGAAAAAACAACTGAAAGTATTTGCCAAAAAAATTGGCGAACTAGAAGTGGGCCAGATGGAGAACA TATCCCCAGGACAGATCATTGATGAACCCATCCGACCAGTCAACATTCCCAGGAAAGAAAAGGATTTCCA AGGGATGCTGGAATACAAGAAGGAGGATGAGCAAAAACTTGTTAAGAACCTGATTCTGGAACTGAAGCCA CGTGGTGTAGCAGTCAATTTGATTCCAGGATTACCGGCATATATCCTGTTCATGTGTGTTCGACATGCTG ACTACCTGAATGATGATCAGAAAGTAAGGTCGTTGCTAACATCAACAATTAACAGCATCAAAAAAGTATT GAAGAAAAGAGGTGATGATTTTGAAACCGTCTCCTTCTGGCTCTCTAACACATGCCGATTTTTGCACTGC TTGAAACAGTACAGTGGAGAAGAGGGCTTTATGAAGCACAACACATCTCGCCAGAATGAACACTGCCTCA CCAATTTTGACCTGGCTGAGTATCGGCAGGTGCTGAGTGACTTGGCCATTCAGATCTACCAGCAGCTCGT GCGGGTGTTAGAGAACATCCTTCAGCCAATGATTGTCTCAGGCATGCTGGAACATGAAACGATTCAGGGC GTGTCTGGGGTGAAGCCCACAGGGTTGAGAAAGCGAACCTCCAGTATCGCCGATGAGGGCACCTACACAC TGGACTCCATCCTCCGGCAGCTCAACTCCTTCCACTCGGTCATGTGTCAGCATGGCATGGACCCTGAACT GATCAAGCAGGTGGTCAAGCAGATGTTCTACATCATAGGGGCCATCACCCTGAACAACCTTCTCCTGCGG AAGGACATGTGCTCCTGGAGTAAAGGCATGCAGATCAGGTACAATGTCAGTCAACTGGAAGAATGGCTGC GTGACAAGAATCTGATGAATAGTGGGGCTAAAGAAACCCTGGAACCTCTCATTCAGGCTGCTCAACTTTT GCAAGTGAAAAAGAAAACAGATGATGATGCAGAAGCCATTTGTTCTATGTGCAATGCTTTAACTACTGCC CAGATTGTGAAAGTGTTGAATTTGTATACTCCAGTTAATGAGTTTGAAGAAAGAGTCTCTGTGTCGTTCA TTCGTACTATACAGATGCGTTTACGAGACAGGAAAGACTCTCCCCAGCTGCTCATGGATGCTAAACACAT CTTTCCTGTCACCTTTCCTTTCAACCCATCTTCCCTCGCACTAGAAACCATCCAGATTCCAGCCAGCCTC GGCCTGGGCTTCATTTCACGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227187 representing NM_001142495 Red=Cloning site Green=Tags(s) MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVG ENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIF AVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAK TTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLG NADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTI PPKHEPLCIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVN QALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFY DNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQ CEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKE HKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSR WTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAAC IRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAA TIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKI EARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKV ATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAK EMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSN ESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKE EERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELD VRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQK RSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVR KLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKP RGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHC LKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQG VSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLR KDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTA QIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASL GLGFISRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001142495
ORF Size	5484 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001142495.1, NP_001135967.1
RefSeq ORF	5487 bp
Locus ID	4644
MW	212.1 kDa
Gene Summary	This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

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