KIF13A (NM_001105568) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA500C11.2; RBKIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226425 representing NM_001105568 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGATACCAAGGTAAAAGTTGCCGTCCGGGTCCGGCCCATGAACCGACGAGAACTGGAACTGAACA CCAAGTGCGTGGTGGAGATGGAAGGGAATCAAACGGTCCTGCACCCTCCTCCTTCTAACACCAAACAGGG AGAAAGGAAACCTCCCAAGGTATTTGCCTTTGATTATTGCTTTTGGTCCATGGATGAATCTAACACTACA AAATACGCTGGTCAAGAAGTGGTTTTCAAGTGCCTTGGGGAAGGAATTCTTGAAAAAGCCTTTCAGGGGT ATAATGCGTGTATTTTTGCATATGGACAGACAGGTTCGGGAAAATCCTTTTCCATGATGGGCCATGCTGA GCAGCTGGGCCTTATTCCAAGGCTCTGCTGTGCTTTATTTAAAAGGATCTCTTTGGAGCAAAATGAGTCA CAGACCTTTAAAGTTGAAGTGTCCTATATGGAAATTTATAATGAGAAAGTTCGGGATCTTTTAGACCCCA AAGGGAGTAGACAGTCTCTTAAAGTTCGAGAACATAAAGTTTTGGGACCATATGTAGATGGTTTATCTCA ACTAGCTGTCACTAGTTTTGAGGATATTGAGTCATTGATGTCTGAGGGAAATAAGTCTCGAACGGTAGCT GCTACCAACATGAACGAAGAAAGCAGCCGCTCCCATGCTGTGTTCAACATCATAATCACACAGACACTTT ATGACCTGCAGTCTGGGAATTCCGGGGAGAAAGTCAGTAAGGTCAGCTTGGTAGACCTGGCGGGTAGCGA AAGAGTATCTAAAACAGGAGCTGCAGGAGAGCGACTGAAAGAAGGCAGCAACATTAACAAATCGCTTACA ACCTTGGGGTTGGTTATATCATCACTGGCTGACCAGGCAGCTGGCAAGGGTAAAAGCAAATTTGTGCCTT ATCGAGATTCAGTCCTCACTTGGCTGCTTAAGGACAACTTGGGGGGCAACAGCCAAACCTCTATGATAGC CACAATCAGCCCAGCCGCAGACAACTATGAAGAGACCCTCTCCACATTAAGATATGCAGACCGAGCCAAA AGGATTGTGAACCATGCTGTTGTGAATGAGGACCCCAACGCAAAAGTGATCCGAGAACTGCGGGAGGAAG TCGAGAAACTGAGAGAGCAGCTCTCTCAGGCAGAGGCCATGAAGGCCCCTGAACTGAAGGAGAAGCTCGA AGAGTCTGAAAAGCTGATAAAAGAACTAACAGTGACTTGGGAAGAGAAGCTGAGAAAAACAGAAGAGATA GCACAGGAAAGACAACGACAACTTGAAAGCATGGGGATTTCCCTGGAGATGTCCGGTATCAAGGTGGGGG ATGACAAATGCTACTTAGTCAATCTGAATGCAGACCCTGCTCTTAACGAACTTCTGGTTTATTATTTAAA GGATCACACCAGGGTGGGTGCAGATACCTCTCAAGATATCCAGCTTTTTGGCATAGGAATTCAGCCTCAG CACTGTGAGATTGACATTGCATCTGATGGAGACGTCACTCTCACTCCAAAAGAAAATGCAAGGTCCTGTG TGAACGGCACCCTTGTGTGCAGTACCACCCAGCTGTGGCATGGTGACCGAATCCTATGGGGAAATAATCA CTTTTTTAGAATAAACTTACCTAAGAGGAAACGTCGAGATTGGTTGAAAGACTTTGAAAAAGAAACGGGC CCGCCAGAGCATGACCTGGATGCAGCCAGTGAGGCTTCCTCTGAACCAGACTATAACTATGAATTTGCAC AGATGGAAGTTATCATGAAAACCCTGAATAGTAATGACCCAGTTCAAAATGTGGTTCAGGTCCTGGAGAA ACAATACCTAGAAGAAAAGAGAAGTGCCCTAGAGGAGCAGCGGCTCATGTATGAGCGGGAACTGGAGCAA CTCCGCCAGCAGCTCTCCCCCGACAGGCAGCCACAGAGTAGCGGCCCTGACCGCCTGGCCTACAGCAGCC AGACAGCGCAGCAGAAGGTGACCCAGTGGGCAGAAGAGAGGGATGAACTCTTCCGACAAAGCCTGGCAAA ACTGCGAGAGCAGCTGGTTAAAGCTAATACCTTGGTGAGGGAAGCAAACTTCCTGGCTGAGGAAATGAGC AAACTCACCGATTACCAAGTGACTCTTCAGATCCCTGCTGCAAACCTCAGTGCCAATAGGAAGAGAGGTG CAATAGTGAGTGAACCAGCTATCCAAGTGAGGAGGAAAGGAAAGAGCACCCAAGTGTGGACCATTGAGAA GCTGGAGAATAAATTAATTGACATGAGAGACCTTTACCAAGAATGGAAGGAAAAAGTTCCTGAGGCAAAG AGACTCTACGGAAAACGAGGTGACCCTTTCTATGAAGCCCAAGAAAATCACAACCTCATCGGGGTGGCGA ATGTATTCTTGGAATGCCTCTTCTGTGATGTGAAACTTCAGTATGCAGTCCCTATCATCAGCCAGCAGGG GGAGGTTGCAGGGCGTCTCCACGTGGAAGTGATGCGTGTTACAGGAGCTGTTCCAGAGCGTGTGGTGGAG GATGACTCTTCGGAGAATTCCAGTGAAAGTGGGAGCCTTGAAGTCGTAGACAGCAGCGGGGAAATCATTC ACCGAGTCAAAAAGCTGACATGTCGGGTAAAAATTAAAGAAGCAACGGGGCTGCCCTTAAACCTCTCAAA TTTTGTCTTCTGTCAATACACATTCTGGGACCAGTGTGAGTCTACGGTGGCTGCCCCGGTGGTGGACCCC GAGGTGCCTTCACCACAGTCCAAGGATGCCCAGTACACAGTGACCTTCTCCCACTGTAAGGACTATGTGG TGAATGTAACAGAAGAATTTCTGGAGTTCATTTCAGATGGAGCACTGGCCATTGAAGTATGGGGCCACCG GTGTGCTGGAAATGGCAGCTCCATCTGGGAGGTCGATTCTCTTCATGCTAAGACAAGAACACTGCATGAC AGGTGGAATGAAGTAACGCGAAGAATAGAAATGTGGATCTCCATATTAGAATTGAATGAGTTAGGAGAGT ATGCTGCAGTGGAACTTCATCAGGCAAAAGATGTCAACACAGGAGGCATCTTTCAACTTAGACAGGGTCA TTCCCGTAGAGTACAAGTCACGGTGAAACCTGTGCAGCATTCAGGGACACTGCCACTTATGGTTGAAGCC ATCCTGTCAGTATCCATCGGCTGTGTAACTGCCAGGTCCACCAAACTCCAAAGAGGGCTGGACAGTTACC AGGAAGAAGACTTAAACTGCGTAAGGGAGAGGTGGTCAGATGCACTCATTAAACGACGAGAATACCTGGA TGAACAGATAAAAAAAGTCAGCAATAAAACAGAGAAAACAGAGGACGATGTGGAGCGGGAAGCCCAGCTT GTGGAGCAGTGGGTAGGGCTGACTGAGGAAAGGAATGCTGTGCTGGTGCCAGCCCCAGGCAGTGGGATTC CTGGGGCACCTGCCGACTGGATCCCACCTCCTGGAATGGAAACCCACATACCAGTTCTCTTCCTCGATTT GAATGCGGATGACCTCAGTGCCAATGAGCAGCTTGTTGGCCCCCATGCATCCGGCGTGAACTCCATCCTG CCCAAGGAGCATGGCAGCCAGTTTTTCTACCTGCCCATCATAAAGCACAGTGATGATGAGGTTTCAGCCA CAGCCTCTTGGGATTCCTCGGTGCATGATTCTGTTCACTTGAATAGGGTCACACCACAGAATGAAAGGAT TTACCTAATTGTGAAAACCACAGTTCAACTCAGCCACCCTGCTGCTATGGAGTTAGTATTACGAAAACGA ATTGCAGCCAATATTTACAACAAACAGAGTTTCACGCAGAGTTTGAAGAGGAGAATATCCCTGAAAAATA TATTTTATTCCTGTGGTGTAACCTATGAAATAGTATCCAATATACCAAAGGCAACTGAGGAGATAGAGGA CCGGGAAACGCTGGCTCTCCTGGCAGCAAGGAGTGAAAACGAAGGCACATCAGATGGGGAGACGTACATT GAGAAGTACACTCGAGGCGTGCTGCAGGTGGAAAACATTCTGAGTCTTGAACGGCTCCGGCAGGCCGTCA CAGTCAAAGAAGCACTTTCCACCAAAGCCCGGCACATTCGGAGGAGCCTCAGTACACCAAATGTTCATAA TGTCTCTTCCAGCCGACCGGACCTTTCTGGCTTTGATGAAGATGACAAGGGTTGGCCAGAGAACCAGTTG GACATGTCTGACTATAGCTCCAGTTACCAAGATGTAGCATGTTATGGAACTTTACCCAGGGATTCTCCTC GAAGGAATAAAGAAGGTTGTACATCAGAGACTCCTCATGCCTTAACCGTCAGCCCTTTTAAAGCATTCTC TCCTCAGCCACCAAAGTTTTTCAAGCCCCTAATGCCTGTAAAAGAGGAGCATAAGAAAAGGATAGCCCTG GAAGCAAGGCCTCTTCTAAGCCAGGAGGACTCTGAGGAGGAAGAAAATGAGCTGGAAGCTATTAACAGGA AGCTAATAAGTTCACAGCCTTATGTACCTGTGGAGTTTGCTGACTTCAGTGTTTACAATGCCAGCTTGGA GAACAGGGAATGGTTTTCCTCTAAAGTAGATCTGTCAAACTCACGGGTCTTGGAGAAAGAAGTGTCCCGT AGCCCTACCACCAGCAGTATTACCAGTGGCTACTTTTCCCACAGTGCCTCCAATGCCACCCTGTCTGACA TGGTGGTCCCTTCTAGTGACAGCTCAGACCAGCTGGCCATTCAGACGAAGGATGCAGACTCCACCGAGCA CTCCACACCATCGCTTGTGCATGATTTCAGGCCGTCCTCAAACAAAGAGTTGACAGAAGTCGAAAAAGGC TTGGTAAAGGACAAGATAATTGTGGTGCCACTCAAGGAAAACAGTGCCTTAGCCAAAGGGAGCCCATCAT CCCAGAGCATCCCTGAGAAAAACTCCAAATCACTGTGCAGGACTGGCTCATGTTCAGAACTAGATGCCTG CCCCAGCAAAATTAGCCAGCCAGCCAGGGGATTCTGCCCCAGGGAGGTGACGGTAGAACACACCACCAAC ATCCTTGAAGACCATTCTTTCACAGAATTTATGGGAGTGTCAGAGGGAAAAGATTTTGATGGTTTGACAG ATTCTTCTGCTGGAGAGCTTTCCAGTAGGAGGAGTCTACCAAATAAAACAGGCGGCAAGACTGTCTCCGA TGGGCTCCACCACCCCAGCCAGCTGCATTCCAAGTTAGAGAATGACCAGGGAGGCACAACCTCACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226425 representing NM_001105568 Red=Cloning site Green=Tags(s) MSDTKVKVAVRVRPMNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTT KYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGHAEQLGLIPRLCCALFKRISLEQNES QTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVA ATNMNEESSRSHAVFNIIITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLT TLGLVISSLADQAAGKGKSKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAK RIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEI AQERQRQLESMGISLEMSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPQ HCEIDIASDGDVTLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETG PPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQ LRQQLSPDRQPQSSGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMS KLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKVPEAK RLYGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERVVE DDSSENSSESGSLEVVDSSGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCESTVAAPVVDP EVPSPQSKDAQYTVTFSHCKDYVVNVTEEFLEFISDGALAIEVWGHRCAGNGSSIWEVDSLHAKTRTLHD RWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGIFQLRQGHSRRVQVTVKPVQHSGTLPLMVEA ILSVSIGCVTARSTKLQRGLDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKTEKTEDDVEREAQL VEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSIL PKEHGSQFFYLPIIKHSDDEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKR IAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALLAARSENEGTSDGETYI EKYTRGVLQVENILSLERLRQAVTVKEALSTKARHIRRSLSTPNVHNVSSSRPDLSGFDEDDKGWPENQL DMSDYSSSYQDVACYGTLPRDSPRRNKEGCTSETPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIAL EARPLLSQEDSEEEENELEAINRKLISSQPYVPVEFADFSVYNASLENREWFSSKVDLSNSRVLEKEVSR SPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLAIQTKDADSTEHSTPSLVHDFRPSSNKELTEVEKG LVKDKIIVVPLKENSALAKGSPSSQSIPEKNSKSLCRTGSCSELDACPSKISQPARGFCPREVTVEHTTN ILEDHSFTEFMGVSEGKDFDGLTDSSAGELSSRRSLPNKTGGKTVSDGLHHPSQLHSKLENDQGGTTSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105568
ORF Size	5247 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105568.3
RefSeq Size	7101 bp
RefSeq ORF	5250 bp
Locus ID	63971
UniProt ID	Q9H1H9
Protein Families	Druggable Genome
MW	196.1 kDa
Gene Summary	This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]

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