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ATP2B2 (NM_001683) Human Tagged ORF Clone
CAT＃: RC223934
- TrueORF®
ATP2B2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2
ORF Plasmid: tGFP
"NM_001683" in other vectors (4)
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CNY 11,970.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PMCA2; PMCA2a; PMCA2i |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223934 representing NM_001683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT TCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATCAA GGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCGGGC ACCGCTCCAGACCTGGAAAAGAGAAAGCAAATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCAAAAA CCTTCCTGCAGCTCGTGTGGGAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCCATCAT CTCCCTGGGGCTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAGGGTGGG GCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCAGTTATCTGTG TGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGGGGCCTGCAGAGCCGCATCGA GCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCTGAGATCGTGGTT GGGGACATAGCCCAGGTCAAATATGGTGACCTCCTCCCTGCCGACGGCCTCTTCATCCAGGGCAATGACC TCAAGATTGATGAAAGCTCCCTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGACAAGGACCCCAT GCTGCTGTCAGGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTGGGTGTGAACTCT CAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA AACAACAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCCGAGGGCGGCGACGCTGACGA CAGGAAGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTGCTGCAGGGCAAGCTCACCAAGCTGGCTGTG CAGATCGGGAAGGCGGGCTTGGTGATGTCAGCCATCACGGTGATCATCCTGGTGCTCTACTTCACTGTGG ACACCTTCGTGGTCAACAAGAAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTGCAGTACTTTGTCAA GTTCTTCATCATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCTCTGGCCGTCACCATC TCGTTGGCCTATTCGGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGA CCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACCAATCGCATGACAGTGGT ACAGGCCTATGTCGGCGACGTCCACTATAAAGAGATCCCCGACCCCAGCTCCATCAACACCAAGACCATG GAGCTGCTGATCAATGCCATCGCCATCAACAGCGCCTACACCACCAAGATTCTGCCCCCAGAGAAGGAGG GCGCCCTGCCTCGGCAGGTGGGCAACAAGACGGAGTGCGGCCTGCTGGGCTTCGTGCTGGACCTGAAGCA GGACTACGAGCCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAAGTGTACACCTTCAACTCCGTG CGCAAGTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGGGCTTCTG AGATCGTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAGCCTCGTGTCTTCCGGCCCCGCGA CCGGGACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCTTGCGATGGGCTCCGCACTATCTGCGTGGCC TACCGCGACTTCCCCAGCAGCCCGGAGCCGGACTGGGACAATGAGAATGACATCCTCAACGAACTCACCT GCATCTGCGTGGTGGGCATCGAGGACCCGGTGCGGCCAGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG GGCAGGCATCACGGTCCGCATGGTCACTGGCGACAATATCAACACGGCTCGGGCCATCGCCATCAAGTGT GGCATCATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGCAAGGAGTTCAACAGGAGGATCCGCAACG AGAAGGGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGGCCAAAGCTGCGGGTGCTGGCTCGCTCCTC CCCAACGGACAAGCATACCCTGGTTAAAGGCATCATCGACAGCACACACACTGAGCAGCGGCAGGTGGTG GCCGTGACGGGGGACGGGACCAACGACGGGCCTGCACTCAAGAAGGCCGACGTGGGCTTCGCCATGGGCA TCGCAGGCACTGACGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGACGACAATTTCAGCAGCATCGT CAAGGCAGTGATGTGGGGCCGCAACGTCTATGACAGCATCTCCAAATTCTTGCAGTTCCAGCTCACCGTC AACGTGGTGGCCGTGATTGTGGCCTTCACAGGCGCCTGCATCACGCAGGACTCCCCTCTGAAGGCCGTGC AGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCGCTGGCACTGGCCACTGAGCCGCCCACGGA GACCCTGCTGCTGAGGAAGCCGTACGGCCGCAACAAGCCGCTCATCTCCAGGACCATGATGAAGAACATC CTGGGCCATGCTGTCTACCAGCTTGCCCTCATCTTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGA TCGACAGCGGGAGGAACGCGCCCCTGCATTCGCCACCCTCAGAACATTACACCATCATCTTCAACACCTT CGTCATGATGCAGCTCTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGC ATCTTCCGGAACCCCATCTTCTGCACCATCGTGCTGGGCACCTTTGCCATCCAGATAGTGATCGTGCAGT TTGGAGGGAAGCCATTCAGCTGCTCTCCACTGCAGCTGGACCAGTGGATGTGGTGCATATTCATTGGGTT AGGAGAGCTCGTTTGGGGCCAGGTCATCGCCACCATCCCGACCAGCAGACTCAAGTTCCTCAAGGAGGCA GGCAGGCTCACACAGAAGGAGGAGATCCCGGAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACG CGGAGCGGGAGCTGCGGCGGGGCCAGATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCG CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCAT AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATTGATGACACCG ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCATCCCTCAACAAGAACAACAGCGC CATCGACAGTGGGATCAACCTGACGACCGACACAAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC ATCCACAGCCTGGAGACGTCGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223934 representing NM_001683
Red=Cloning site Green=Tags(s) MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPG TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGG AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV GDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNS QTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAV QIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTI SLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTM ELLINAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSV RKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVA YRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKC GIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVV AVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTV NVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNI LGHAVYQLALIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDG IFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEA GRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIH NFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSP IHSLETSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001683 |
| ORF Size | 3594 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001683.5 |
| RefSeq Size | 8827 bp |
| RefSeq ORF | 3597 bp |
| Locus ID | 491 |
| Domains | E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway |
| MW | 132.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223934L3 | Lenti-ORF clone of ATP2B2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2 |
CNY 13,870.00 |
|
| RC223934L4 | Lenti-ORF clone of ATP2B2 (mGFP-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2 |
CNY 13,870.00 |
|
| RG223934 | ATP2B2 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2 |
CNY 13,210.00 |
|
| SC119107 | ATP2B2 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2 |
CNY 8,352.00 |
