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ABCB4 (NM_000443) Human Tagged ORF Clone
CAT＃: RC222574
- TrueORF®
ABCB4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A
ORF Plasmid: tGFP
"NM_000443" in other vectors (6)
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CNY 13,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3; PFIC-3; PGY3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222574 representing NM_000443
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTTGAGGCGGCAAAGAACGGAACAGCCTGGCGCCCCACGAGCGCGGAGGGCGACTTTGAACTGG GCATCAGCAGCAAACAAAAAAGGAAAAAAACGAAGACAGTGAAAATGATTGGAGTATTAACATTGTTTCG ATACTCCGATTGGCAGGATAAATTGTTTATGTCGCTGGGTACCATCATGGCCATAGCTCACGGATCAGGT CTCCCCCTCATGATGATAGTATTTGGAGAGATGACTGACAAATTTGTTGATACTGCAGGAAACTTCTCCT TTCCAGTGAACTTTTCCTTGTCGCTGCTAAATCCAGGCAAAATTCTGGAAGAAGAAATGACTAGATATGC ATATTACTACTCAGGATTGGGTGCTGGAGTTCTTGTTGCTGCCTATATACAAGTTTCATTTTGGACTTTG GCAGCTGGTCGACAGATCAGGAAAATTAGGCAGAAGTTTTTTCATGCTATTCTACGACAGGAAATAGGAT GGTTTGACATCAATGACACCACTGAACTCAATACGCGGCTAACAGATGACATCTCCAAAATCAGTGAAGG AATTGGTGACAAGGTTGGAATGTTCTTTCAAGCAGTAGCCACGTTTTTTGCAGGATTCATAGTGGGATTC ATCAGAGGATGGAAGCTCACCCTTGTGATAATGGCCATCAGCCCTATTCTAGGACTCTCTGCAGCCGTTT GGGCAAAGATACTCTCGGCATTTAGTGACAAAGAACTAGCTGCTTATGCAAAAGCAGGCGCCGTGGCAGA AGAGGCTCTGGGGGCCATCAGGACTGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTGGAAAGGTATCAG AAACATTTAGAAAATGCCAAAGAGATTGGAATTAAAAAAGCTATTTCAGCAAACATTTCCATGGGTATTG CCTTCCTGTTAATATATGCATCATATGCACTGGCCTTCTGGTATGGATCCACTCTAGTCATATCAAAAGA ATATACTATTGGAAATGCAATGACAGTTTTTTTTTCAATCCTAATTGGAGCTTTCAGTGTTGGCCAGGCT GCCCCATGTATTGATGCTTTTGCCAATGCAAGAGGAGCAGCATATGTGATCTTTGATATTATTGATAATA ATCCTAAAATTGACAGTTTTTCAGAGAGAGGACACAAACCAGACAGCATCAAAGGGAATTTGGAGTTCAA TGATGTTCACTTTTCTTACCCTTCTCGAGCTAACGTCAAGATCTTGAAGGGCCTCAACCTGAAGGTGCAG AGTGGGCAGACGGTGGCCCTGGTTGGAAGTAGTGGCTGTGGGAAGAGCACAACGGTCCAGCTGATACAGA GGCTCTATGACCCTGATGAGGGCACAATTAACATTGATGGGCAGGATATTAGGAACTTTAATGTAAACTA TCTGAGGGAAATCATTGGTGTGGTGAGTCAGGAGCCGGTGCTGTTTTCCACCACAATTGCTGAAAATATT TGTTATGGCCGTGGAAATGTAACCATGGATGAGATAAAGAAAGCTGTCAAAGAGGCCAACGCCTATGAGT TTATCATGAAATTACCACAGAAATTTGACACCCTGGTTGGAGAGAGAGGGGCCCAGCTGAGTGGTGGGCA GAAGCAGAGGATCGCCATTGCACGTGCCCTGGTTCGCAACCCCAAGATCCTTCTGCTGGATGAGGCCACG TCAGCATTGGACACAGAAAGTGAAGCTGAGGTACAGGCAGCTCTGGATAAGGCCAGAGAAGGCCGGACCA CCATTGTGATAGCACACCGACTGTCTACGGTCCGAAATGCAGATGTCATCGCTGGGTTTGAGGATGGAGT AATTGTGGAGCAAGGAAGCCACAGCGAACTGATGAAGAAGGAAGGGGTGTACTTCAAACTTGTCAACATG CAGACATCAGGAAGCCAGATCCAGTCAGAAGAATTTGAACTAAATGATGAAAAGGCTGCCACTAGAATGG CCCCAAATGGCTGGAAATCTCGCCTATTTAGGCATTCTACTCAGAAAAACCTTAAAAATTCACAAATGTG TCAGAAGAGCCTTGATGTGGAAACCGATGGACTTGAAGCAAATGTGCCACCAGTGTCCTTTCTGAAGGTC CTGAAACTGAATAAAACAGAATGGCCCTACTTTGTCGTGGGAACAGTATGTGCCATTGCCAATGGGGGGC TTCAGCCGGCATTTTCAGTCATATTCTCAGAGATCATAGCGATTTTTGGACCAGGCGATGATGCAGTGAA GCAGCAGAAGTGCAACATATTCTCTTTGATTTTCTTATTTCTGGGAATTATTTCTTTTTTTACTTTCTTC CTTCAGGGTTTCACGTTTGGGAAAGCTGGCGAGATCCTCACCAGAAGACTGCGGTCAATGGCTTTTAAAG CAATGCTAAGACAGGACATGAGCTGGTTTGATGACCATAAAAACAGTACTGGTGCACTTTCTACAAGACT TGCCACAGATGCTGCCCAAGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAATATAGCT AACCTTGGAACTGGTATTATCATATCATTTATCTACGGTTGGCAGTTAACCCTATTGCTATTAGCAGTTG TTCCAATTATTGCTGTGTCAGGAATTGTTGAAATGAAATTGTTGGCTGGAAATGCCAAAAGAGATAAAAA AGAACTGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTAGGACAGTTGTGTCTTTGACC CAGGAAAGAAAATTTGAATCAATGTATGTTGAAAAATTGTATGGACCTTACAGGAATTCTGTGCAGAAGG CACACATCTATGGAATTACTTTTAGTATCTCACAAGCATTTATGTATTTTTCCTATGCCGGTTGTTTTCG ATTTGGTGCATATCTCATTGTGAATGGACATATGCGCTTCAGAGATGTTATTCTGGTGTTTTCTGCAATT GTATTTGGTGCAGTGGCTCTAGGACATGCCAGTTCATTTGCTCCAGACTATGCTAAAGCTAAGCTGTCTG CAGCCCACTTATTCATGCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGTGAAGAGGGGCTGAAGCC TGATAAATTTGAAGGAAATATAACATTTAATGAAGTCGTGTTCAACTATCCCACCCGAGCAAACGTGCCA GTGCTTCAGGGGCTGAGCCTGGAGGTGAAGAAAGGCCAGACACTAGCCCTGGTGGGCAGCAGTGGCTGTG GGAAGAGCACGGTGGTCCAGCTCCTGGAGCGGTTCTACGACCCCTTGGCGGGGACAGTGCTTCTCGATGG TCAAGAAGCAAAGAAACTCAATGTCCAGTGGCTCAGAGCTCAACTCGGAATCGTGTCTCAGGAGCCTATC CTATTTGACTGCAGCATTGCCGAGAATATTGCCTATGGAGACAACAGCCGGGTTGTATCACAGGATGAAA TTGTGAGTGCAGCCAAAGCTGCCAACATACATCCTTTCATCGAGACGTTACCCCACAAATATGAAACAAG AGTGGGAGATAAGGGGACTCAGCTCTCAGGAGGTCAAAAACAGAGGATTGCTATTGCCCGAGCCCTCATC AGACAACCTCAAATCCTCCTGTTGGATGAAGCTACATCAGCTCTGGATACTGAAAGTGAAAAGGTTGTCC AAGAAGCCCTGGACAAAGCCAGAGAAGGCCGCACCTGCATTGTGATTGCTCACCGCCTGTCCACCATCCA GAATGCAGACTTAATAGTGGTGTTTCAGAATGGGAGAGTCAAGGAGCATGGCACGCATCAGCAGCTGCTG GCACAGAAAGGCATCTATTTTTCAATGGTCAGTGTCCAGGCTGGGACACAGAACTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222574 representing NM_000443
Red=Cloning site Green=Tags(s) MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSG LPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTL AAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGF IRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQ KHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQA APCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQ SGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENI CYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEAT SALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNM QTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLDVETDGLEANVPPVSFLKV LKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFF LQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIA NLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLT QERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAI VFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVP VLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPI LFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALI RQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLL AQKGIYFSMVSVQAGTQNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000443 |
| ORF Size | 3837 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000443.2, NP_000434.1 |
| RefSeq Size | 5764 bp |
| RefSeq ORF | 3840 bp |
| Locus ID | 5244 |
| UniProt ID | P21439 |
| Domains | ABC_membrane, ABC_tran, AAA |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | ABC transporters |
| MW | 140.5 kDa |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222574L1 | Lenti-ORF clone of ABCB4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 14,630.00 |
|
| RC222574L2 | Lenti-ORF clone of ABCB4 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 14,630.00 |
|
| RC222574L3 | Lenti-ORF clone of ABCB4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 14,630.00 |
|
| RC222574L4 | Lenti-ORF clone of ABCB4 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 14,630.00 |
|
| RG222574 | ABCB4 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 13,970.00 |
|
| SC309931 | ABCB4 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A |
CNY 24,700.00 |
