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RAPGEF2 (NM_014247) Human Tagged Lenti ORF Clone
CAT＃: RC222055L4
- LentiORF®
Lenti-ORF clone of RAPGEF2 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2)
"NM_014247" in other vectors (4)
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CNY 12,730.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | CNrasGEF; NRAPGEP; nRap GEP; PDZ-GEF1; PDZGEF1; RA-GEF; RA-GEF-1; RAGEF; Rap-GEP |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC222055).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014247 |
| ORF Size | 4497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014247.1, NP_055062.1 |
| RefSeq Size | 6568 bp |
| RefSeq ORF | 4500 bp |
| Locus ID | 9693 |
| UniProt ID | Q9Y4G8 |
| Domains | RA, cNMP, RasGEFN, PDZ, RasGEF |
| Protein Pathways | MAPK signaling pathway |
| MW | 167.2 kDa |
| Gene Summary | Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222055 | RAPGEF2 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2) |
CNY 10,256.00 |
|
| RC222055L3 | Lenti-ORF clone of RAPGEF2 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2) |
CNY 12,730.00 |
|
| RG222055 | RAPGEF2 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2) |
CNY 11,880.00 |
|
| SC315368 | RAPGEF2 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2) |
CNY 28,880.00 |
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