PDE12 (NM_177966) Human Tagged Lenti ORF Clone
CAT＃: RC217016L3
- LentiORF®
Lenti ORF clone of Human phosphodiesterase 12 (PDE12), Myc-DDK-tagged
"NM_177966" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7320.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2'-PDE; 2-PDE |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC217016).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_177966 |
| ORF Size | 1827 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177966.4 |
| RefSeq Size | 3923 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 201626 |
| UniProt ID | Q6L8Q7 |
| MW | 67.2 kDa |
| Gene Summary | Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. Degrades triphosphorylated 2-5A to produce AMP and ATP (PubMed:26055709). Also cleaves 3',5'-phosphodiester bond of oligoadenylates (PubMed:21666256, PubMed:30389976, PubMed:26055709). Plays a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme increases cellular 2-5A levels and decreases viral replication in cultured small-airway epithelial cells and Hela cells (PubMed:26055709).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217016 | PDE12 (Myc-DDK-tagged)-Human phosphodiesterase 12 (PDE12) |
CNY 6800.00 |
|
| RC217016L1 | Lenti ORF clone of Human phosphodiesterase 12 (PDE12), Myc-DDK-tagged |
CNY 9200.00 |
|
| RC217016L2 | Lenti ORF clone of Human phosphodiesterase 12 (PDE12), mGFP tagged |
CNY 9200.00 |
|
| RC217016L4 | Lenti ORF clone of Human phosphodiesterase 12 (PDE12), mGFP tagged |
CNY 7320.00 |
|
| RG217016 | PDE12 (tGFP-tagged) - Human phosphodiesterase 12 (PDE12) |
CNY 8400.00 |
|
| SC314189 | PDE12 (untagged)-Human phosphodiesterase 12 (PDE12) |
CNY 6808.00 |
