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PDE5A (NM_033437) Human Tagged ORF Clone
CAT＃: RC216759
- TrueORF®
PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3
ORF Plasmid: tGFP
"NM_033437" in other vectors (4)
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CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGB-PDE; CN5A; PDE5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216759 representing NM_033437
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACA CCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGCCCCTGGAACACCAACCAG GAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTG AGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAG GGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATG TCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAA GACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAG TTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCC CTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAG ACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGTGTAGCCCAGGCCATCA ACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTGCTGCTTATTTGGCATT TTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTG CTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTG CCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTC TTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGGGAACAT GATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAATACTATGGAACCACTTAATATCCCAG ATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAAACCAGCAGTGCATTAG AAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAAT AAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTG TCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCA AATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTACAGTCG TTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGCTTCAGTGACTTTGAGC TGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACCTTGTGCAGAACTTCCA GATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTAT CATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGTTTGCTGCTCTAAAAGCAGGCAAAATTCAGA ACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGG TGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATCATGGAA CACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATTCTCAGTGGCCTCTCCA TTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAGACCTAGCACTGTACAT TAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTCAATTTGGAAGATCCTCATCAAAAG GAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAAC AACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACAT AGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTCATAGAT GCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTCCCTTTGCTAGATGGCT GCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGCTGATTAATGGGGAAAG CGGCCAGGCCAAGCGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216759 representing NM_033437
Red=Cloning site Green=Tags(s) MVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTV SFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCE DSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYK TQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQV LLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREH DANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVN KMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQS LAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAY HNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIME HHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQK ELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFID AICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033437 |
| ORF Size | 2469 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033437.3, NP_246273.2 |
| RefSeq Size | 6788 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 8654 |
| UniProt ID | O76074 |
| Domains | PDEase, GAF |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 93.6 kDa |
| Gene Summary | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216759L3 | Lenti-ORF clone of PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CNY 10,070.00 |
|
| RC216759L4 | Lenti-ORF clone of PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CNY 10,070.00 |
|
| RG216759 | PDE5A (tGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CNY 9,030.00 |
|
| SC100003 | PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3 |
CNY 6,032.00 |
