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ERVWE1 (ERVW-1) (NM_014590) Human Tagged ORF Clone
CAT＃: RC213951
- TrueORF®
ERVW (Myc-DDK-tagged)-Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1
ORF Plasmid: tGFP
"NM_014590" in other vectors (6)
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CNY 6,008.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213951 representing NM_014590
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTCCCTTATCATATTTTTCTCTTTACTGTTCTTTTACCCTCTTTCACTCTCACTGCACCCCCTC CATGCCGCTGTATGACCAGTAGCTCCCCTTACCAAGAGTTTCTATGGAGAATGCAGCGTCCCGGAAATAT TGATGCCCCATCGTATAGGAGTCTTTCTAAGGGAACCCCCACCTTCACTGCCCACACCCATATGCCCCGC AACTGCTATCACTCTGCCACTCTTTGCATGCATGCAAATACTCATTATTGGACAGGAAAAATGATTAATC CTAGTTGTCCTGGAGGACTTGGAGTCACTGTCTGTTGGACTTACTTCACCCAAACTGGTATGTCTGATGG GGGTGGAGTTCAAGATCAGGCAAGAGAAAAACATGTAAAAGAAGTAATCTCCCAACTCACCCGGGTACAT GGCACCTCTAGCCCCTACAAAGGACTAGATCTCTCAAAACTACATGAAACCCTCCGTACCCATACTCGCC TGGTAAGCCTATTTAATACCACCCTCACTGGGCTCCATGAGGTCTCGGCCCAAAACCCTACTAACTGTTG GATATGCCTCCCCCTGAACTTCAGGCCATATGTTTCAATCCCTGTACCTGAACAATGGAACAACTTCAGC ACAGAAATAAACACCACTTCCGTTTTAGTAGGACCTCTTGTTTCCAATCTGGAAATAACCCATACCTCAA ACCTCACCTGTGTAAAATTTAGCAATACTACATACACAACCAACTCCCAATGCATCAGGTGGGTAACTCC TCCCACACAAATAGTCTGCCTACCCTCAGGAATATTTTTTGTCTGTGGTACCTCAGCCTATCGTTGTTTG AATGGCTCTTCAGAATCTATGTGCTTCCTCTCATTCTTAGTGCCCCCTATGACCATCTACACTGAACAAG ATTTATACAGTTATGTCATATCTAAGCCCCGCAACAAAAGAGTACCCATTCTTCCTTTTGTTATAGGAGC AGGAGTGCTAGGTGCACTAGGTACTGGCATTGGCGGTATCACAACCTCTACTCAGTTCTACTACAAACTA TCTCAAGAACTAAATGGGGACATGGAACGGGTCGCCGACTCCCTGGTCACCTTGCAAGATCAACTTAACT CCCTAGCAGCAGTAGTCCTTCAAAATCGAAGAGCTTTAGACTTGCTAACCGCTGAAAGAGGGGGAACCTG TTTATTTTTAGGGGAAGAATGCTGTTATTATGTTAATCAATCCGGAATCGTCACTGAGAAAGTTAAAGAA ATTCGAGATCGAATACAACGTAGAGCAGAGGAGCTTCGAAACACTGGACCCTGGGGCCTCCTCAGCCAAT GGATGCCCTGGATTCTCCCCTTCTTAGGACCTCTAGCAGCTATAATATTGCTACTCCTCTTTGGACCCTG TATCTTTAACCTCCTTGTTAACTTTGTCTCTTCCAGAATCGAAGCTGTAAAACTACAAATGGAGCCCAAG ATGCAGTCCAAGACTAAGATCTACCGCAGACCCCTGGACCGGCCTGCTAGCCCACGATCTGATGTTAATG ACATCAAAGGCACCCCTCCTGAGGAAATCTCAGCTGCACAACCTCTACTACGCCCCAATTCAGCAGGAAG CAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213951 representing NM_014590
Red=Cloning site Green=Tags(s) MALPYHIFLFTVLLPSFTLTAPPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPR NCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVH GTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFS TEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCL NGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKL SQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKE IRDRIQRRAEELRNTGPWGLLSQWMPWILPFLGPLAAIILLLLFGPCIFNLLVNFVSSRIEAVKLQMEPK MQSKTKIYRRPLDRPASPRSDVNDIKGTPPEEISAAQPLLRPNSAGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014590 |
| ORF Size | 1614 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014590.3, NP_055405.3 |
| RefSeq Size | 3044 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 30816 |
| UniProt ID | Q9UQF0 |
| Domains | ENV_polyprotein |
| Protein Families | Transmembrane |
| MW | 59.87 kDa |
| Gene Summary | Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Interferon-Induced Transmembrane Proteins Inhibit Cell Fusion Mediated by Trophoblast Syncytins
,Zani, A;Zhang, L;McMichael, TM;Kenney, AD;Chemudupati, M;Kwiek, JJ;Liu, SL;Yount, JS;,
J. Biol. Chem.
,PubMed ID 31735710
[ERVW-1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213951L1 | Lenti ORF clone of Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213951L2 | Lenti ORF clone of Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1, mGFP tagged |
CNY 8,408.00 |
|
| RC213951L3 | Lenti ORF clone of Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213951L4 | Lenti ORF clone of Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG213951 | ERVW (tGFP-tagged) - Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1 |
CNY 7,608.00 |
|
| SC114945 | ERVW (untagged)-Human endogenous retrovirus group W, member 1 (ERVW-1), transcript variant 1 |
CNY 6,016.00 |
