Estrogen Receptor 1 (ESR1) (NM_000125) Human Tagged ORF Clone

CAT＃: RC213277

TrueORF^®

ESR1 (Myc-DDK-tagged)-Human estrogen receptor 1 (ESR1), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_000125" in other vectors (6)

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CNY 6,648.00

货期*

现货

规格

Cited in 13 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

参与“2024逐梦开学季·科研添动力”活动,可提供10 µL试用规格

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA503759

ESR1 (Estrogen Receptor 1/ER) mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)

CNY 1,999.00
CNY 2,700.00

参与“2024逐梦开学季·科研添动力”活动,可提供10 µL试用规格

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ER; Era; ESR; ESRA; ESTRR; NR3A1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213277 representing NM_000125 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGACCCTCCACACCAAAGCATCCGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGG AGCCCCTGAACCGTCCGCAGCTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAG CAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCGGCCGCCGCCAACGCG CAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGCTCCAACGGCC TGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCT GTCGCCTTTCCTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACG GTGCGCGAGGCCGGCCCGCCGGCATTCTACAGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAA GATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAGGAGACTCGCTACTGTGCAGT GTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAG AGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGA GGAAGAGCTGCCAGGCCTGCCGGCTCCGCAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAA AGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAGAGAGATGATGGGGAGGGCAGGGGTGAAGTG GGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGCTCTAAGAAGA ACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCCATACT CTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCA GACAGGGAGCTGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGGCTTTGTGGATTTGACCCTCCATG ATCAGGTCCACCTTCTAGAATGTGCCTGGCTAGAGATCCTGATGATTGGTCTCGTCTGGCGCTCCATGGA GCACCCAGGGAAGCTACTGTTTGCTCCTAACTTGCTCTTGGACAGGAACCAGGGAAAATGTGTAGAGGGC ATGGTGGAGATCTTCGACATGCTGCTGGCTACATCATCTCGGTTCCGCATGATGAATCTGCAGGGAGAGG AGTTTGTGTGCCTCAAATCTATTATTTTGCTTAATTCTGGAGTGTACACATTTCTGTCCAGCACCCTGAA GTCTCTGGAAGAGAAGGACCATATCCACCGAGTCCTGGACAAGATCACAGACACTTTGATCCACCTGATG GCCAAGGCAGGCCTGACCCTGCAGCAGCAGCACCAGCGGCTGGCCCAGCTCCTCCTCATCCTCTCCCACA TCAGGCACATGAGTAACAAAGGCATGGAGCATCTGTACAGCATGAAGTGCAAGAACGTGGTGCCCCTCTA TGACCTGCTGCTGGAGATGCTGGACGCCCACCGCCTACATGCGCCCACTAGCCGTGGAGGGGCATCCGTG GAGGAGACGGACCAAAGCCACTTGGCCACTGCGGGCTCTACTTCATCGCATTCCTTGCAAAAGTATTACA TCACGGGGGAGGCAGAGGGTTTCCCTGCCACGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC213277 representing NM_000125 Red=Cloning site Green=Tags(s) MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_000125
ORF Size	1785 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000125.2, NP_000116.2
RefSeq Size	6456 bp
RefSeq ORF	1788 bp
Locus ID	2099
UniProt ID	P03372
Domains	HOLI, Oest_recep, zf-C4
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
MW	66 kDa
Gene Summary	This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain. [provided by RefSeq, Jul 2020]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (13)

The use of this cDNA Clones has been cited in the following citations:
The crosstalk between IGF-1R and ER-α in the proliferation and anti-inflammation of nucleus pulposus cells ,Chen, RS;Zhang, XB;Zhu, XT;Wang, CS;, Eur Rev Med Pharmacol Sci ,PubMed ID 32572901 [Estrogen Receptor 1]
Receptor-binding affinities of bisphenol A and its next-generation analogs for human nuclear receptors ,Liu, X;Sakai, H;Nishigori, M;Suyama, K;Nawaji, T;Ikeda, S;Nishigouchi, M;Okada, H;Matsushima, A;Nose, T;Shimohigashi, M;Shimohigashi, Y;, Toxicol. Appl. Pharmacol. ,PubMed ID 31195007 [Estrogen Receptor 1]
ERα upregulates the expression of long non-coding RNA LINC00472 which suppresses the phosphorylation of NF-κB in breast cancer ,Wang, Z;Katsaros, D;Biglia, N;Shen, Y;Loo, L;Yu, X;Lin, H;Fu, Y;Chu, WM;Fei, P;Ni, Y;Jia, W;Deng, X;Qian, B;Yu, H;, Breast Cancer Res. Treat. ,PubMed ID 30830488 [Estrogen Receptor 1]
Polyfluorinated iodine alkanes regulated distinct breast cancer cell progression through binding with estrogen receptor alpha or beta isoforms ,Song, W;Liu, QS;Sun, Z;Yang, X;Zhou, Q;Jiang, G;, Environ. Pollut. ,PubMed ID 29665550 [Estrogen Receptor 1]
Estrogen receptor regulates hormone-induced growth arrest in a luminal A like breast cancer model ,Haddon, L;Hu, S;Jabbari, H;Loney, B;Lichtensztejn, Z;Fahlman, R;Hitt, M;McManus, K;Dabbs, K;Mackey, J;Hugh, J;, bioRxiv [Estrogen Receptor 1]
ER alpha binding by transcription factors NFIB and YBX1 enables FGFR2 signalling to modulate estrogen responsiveness in breast cancer ,Campbell, TM;Castro, MA;deOliveira, KG;Ponder, BA;Meyer, KB;, Cancer Res.2017 ,PubMed ID 29180470 [Estrogen Receptor 1]
RIZ1 is regulated by estrogen and suppresses tumor progression in endometrial cancer ,Yang, T;Ren, C;Jiang, A;Yu, Z;Li, G;Wang, G;Zhang, Q;, Biochem. Biophys. Res. Commun. ,PubMed ID 28528974 [Estrogen Receptor 1]
Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer ,Hamilton, DH;Griner, LM;Keller, JM;Hu, X;Southall, N;Marugan, J;David, JM;Ferrer, M;Palena, C;, Clin. Cancer Res ,PubMed ID 27267852 [Estrogen Receptor 1]
Synthesis and structure-activity relationships of 1-benzylindane derivatives as selective agonists for estrogen receptor beta ,Yonekubo, S;Fushimi, N;Miyagi, T;Nakanishi, O;Katsuno, K;Ozawa, M;Handa, C;Furuya, N;Muranaka, H;, Bioorg. Med. Chem. ,PubMed ID 27692995 [Estrogen Receptor 1]
Let-7c blocks estrogen-activated Wnt signaling in induction of self-renewal of breast cancer stem cells ,Sun, X;Xu, C;Tang, SC;Wang, J;Wang, H;Wang, P;Du, N;Qin, S;Li, G;Xu, S;Tao, Z;Liu, D;Ren, H;, Cancer Gene Ther. ,PubMed ID 26987290 [Estrogen Receptor 1]
Estrogen receptor alpha inhibits RLR-mediated immune response via ubiquitinating TRAF3 ,Wang, C;Huang, Y;Sheng, J;Huang, H;Zhou, J;, Cell. Signal. ,PubMed ID 26186972 [Estrogen Receptor 1]
Estrogen receptor a enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner ,Cao, P;Feng, F;Dong, G;Yu, C;Feng, S;Song, E;Shi, G;Liang, Y;Liang, G;, BMC Cancer ,PubMed ID 26122040 [Estrogen Receptor 1]
?9-Tetrahydrocannabinol Disrupts Estrogen-Signaling through Up-Regulation of Estrogen Receptor β (ERβ) ,null, Chemical research in toxicology ,PubMed ID 23718638 [Estrogen Receptor 1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC213277L1	Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,048.00
RC213277L2	Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,048.00
RC213277L3	Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,048.00
RC213277L4	Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,048.00
RG213277	ESR1 (tGFP-tagged) - Human estrogen receptor 1 (ESR1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
SC125287	ESR1 (untagged)-Human estrogen receptor 1 (ESR1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,656.00