NFYA (NM_002505) Human Tagged ORF Clone
CAT#: RC213263
NFYA (Myc-DDK-tagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1
ORF Plasmid: tGFP
"NM_002505" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CBF-A; CBF-B; HAP2; NF-YA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213263 representing NM_002505
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGC AGCAGCAGCAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAGGTGGCATCCGCCTCAGGCCA GCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATC ACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTAC CTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGG ACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCT GTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTG AAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCTGT TTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACC AACACAACCAGCAGTGGGCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAG GAGGGATGGTCATGATGGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGC AGAGATGCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAA GCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCATGAGTCTCGGC ACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTTCTCTCCAAAGGAAAAGGATAGTCC CCATATGCAGGATCCAAACCAAGCCGATGAAGAAGCAATGACACAGATCATCCGAGTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213263 representing NM_002505
Red=Cloning site Green=Tags(s) MEQYTANSNSSTEQIVVQAGQIQQQQQGGVTAVQLQTEAQVASASGQQVQTLQVVQGQPLMVQVSGGQLI TSTGQPIMVQAVPGGQGQTIMQVPVSGTQGLQQIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTA VTAGQTQTQQQIAVQGQQVAQTAEGQTIVYQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGANT NTTSSGQGTVTVTLPVAGNVVNSGGMVMMVPGAGSVPAIQRIPLPGAEMLEEEPLYVNAKQYHRILKRRQ ARAKLEAEGKIPKERRKYLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002505 |
ORF Size | 1041 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002505.5 |
RefSeq Size | 2834 bp |
RefSeq ORF | 1044 bp |
Locus ID | 4800 |
UniProt ID | P23511 |
Domains | CBF |
Protein Families | Transcription Factors |
Protein Pathways | Antigen processing and presentation |
MW | 36.7 kDa |
Gene Summary | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213263L1 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC213263L2 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC213263L3 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC213263L4 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG213263 | NFYA (tGFP-tagged) - Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1 |
CNY 5,256.00 |
|
SC118595 | NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1 |
CNY 3,656.00 |