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KCNJ5 (NM_000890) Human Tagged ORF Clone
CAT＃: RC210184
KCNJ5 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5)
ORF Plasmid: tGFP
"NM_000890" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIR; GIRK4; KATP1; KIR3.4; LQT13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210184 representing NM_000890
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCGATTCTAGGAATGCCATGAACCAGGACATGGAGATTGGAGTCACTCCCTGGGACCCCAAGA AGATTCCAAAACAGGCCCGCGATTATGTCCCCATTGCCACAGACCGTACGCGCCTGCTGGCCGAGGGCAA GAAACCACGCCAGCGCTACATGGAGAAGAGCGGCAAGTGCAATGTGCACCACGGCAACGTCCAGGAGACC TACCGGTACCTGAGTGACCTCTTCACCACCCTGGTGGACCTCAAGTGGCGCTTCAACTTGCTCGTCTTCA CCATGGTTTACACTGTCACCTGGCTGTTCTTCGGCTTCATTTGGTGGCTCATTGCTTATATCCGGGGTGA CCTGGACCATGTTGGCGACCAAGAGTGGATTCCTTGTGTTGAAAACCTCAGTGGCTTCGTGTCCGCTTTC CTGTTCTCCATTGAGACCGAAACAACCATTGGGTATGGCTTCCGAGTCATCACAGAGAAGTGTCCAGAGG GGATTATACTCCTCTTGGTCCAGGCCATCCTGGGCTCCATCGTCAATGCCTTCATGGTGGGGTGCATGTT TGTCAAGATCAGCCAGCCCAAGAAGAGAGCGGAGACCCTCATGTTTTCCAACAACGCAGTCATCTCCATG CGGGACGAGAAGCTGTGCCTCATGTTCCGGGTGGGCGACCTCCGCAACTCCCACATCGTGGAGGCCTCCA TCCGGGCCAAGCTCATCAAGTCCCGGCAGACCAAAGAGGGGGAGTTCATCCCCCTGAACCAGACAGACAT CAACGTGGGCTTTGACACGGGCGACGACCGCCTCTTCCTGGTGTCTCCTCTGATCATCTCCCACGAGATC AACGAGAAGAGCCCTTTCTGGGAGATGTCTCAGGCTCAGCTGCATCAGGAAGAGTTTGAAGTTGTGGTCA TTCTAGAAGGGATGGTGGAAGCCACAGGCATGACCTGCCAAGCCCGGAGCTCCTACATGGATACAGAGGT GCTCTGGGGCCACCGATTCACACCAGTCCTCACCTTGGAAAAGGGCTTCTATGAGGTGGACTACAACACC TTCCATGATACCTATGAGACCAACACACCCAGCTGCTGTGCCAAGGAGCTGGCAGAAATGAAGAGGGAAG GCCGGCTCCTCCAGTACCTCCCCAGCCCCCCACTGCTGGGGGGCTGTGCTGAGGCAGGGCTGGATGCAGA GGCTGAGCAGAATGAAGAAGATGAGCCCAAGGGGCTGGGTGGGTCCAGGGAGGCCAGGGGCTCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210184 representing NM_000890
Red=Cloning site Green=Tags(s) MAGDSRNAMNQDMEIGVTPWDPKKIPKQARDYVPIATDRTRLLAEGKKPRQRYMEKSGKCNVHHGNVQET YRYLSDLFTTLVDLKWRFNLLVFTMVYTVTWLFFGFIWWLIAYIRGDLDHVGDQEWIPCVENLSGFVSAF LFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISM RDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEI NEKSPFWEMSQAQLHQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNT FHDTYETNTPSCCAKELAEMKREGRLLQYLPSPPLLGGCAEAGLDAEAEQNEEDEPKGLGGSREARGSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000890 |
| ORF Size | 1257 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000890.5 |
| RefSeq Size | 2912 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 3762 |
| UniProt ID | P48544 |
| Domains | IRK |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 47.5 kDa |
| Gene Summary | This gene encodes an integral membrane protein which belongs to one of seven subfamilies of inward-rectifier potassium channel proteins called potassium channel subfamily J. The encoded protein is a subunit of the potassium channel which is homotetrameric. It is controlled by G-proteins and has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Naturally occurring mutations in this gene are associated with aldosterone-producing adenomas. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210184L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210184L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5), mGFP tagged |
CNY 5,890.00 |
|
| RC210184L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210184L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5), mGFP tagged |
CNY 5,890.00 |
|
| RG210184 | KCNJ5 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5) |
CNY 7,088.00 |
|
| SC119590 | KCNJ5 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5) |
CNY 2,950.00 |
