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DHX32 (NM_018180) Human Tagged ORF Clone
CAT＃: RC209736
DHX32 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32)
ORF Plasmid: tGFP
"NM_018180" in other vectors (4)
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CNY 5,440.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DDX32; DHLP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209736 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAGAAGGGCTGGAGTGTCCAAACTCTTCCTCTGAAAAACGCTATTTTCCTGAATCCCTGGATT CCAGCGATGGGGATGAGGAAGAGGTTTTGGCCTGTGAGGATTTGGAACTTAACCCCTTTGATGGATTGCC ATATTCATCACGTTATTATAAACTTCTGAAAGAAAGAGAAGATCTTCCTATATGGAAAGAAAAATACTCC TTTATGGAGAACCTGCTTCAAAATCAAATCGTGATTGTTTCAGGAGATGCTAAATGTGGTAAGAGCGCTC AGGTTCCTCAGTGGTGTGCTGAATATTGTCTTTCCATCCACTACCAGCACGGGGGCGTGATATGCACACA GGTCCACAAGCAGACTGTGGTCCAGCTCGCCCTGCGGGTGGCGGATGAAATGGATGTTAACATTGGTCAT GAGGTTGGCTACGTGATCCCTTTCGAGAACTGCTGTACCAACGAAACAATCCTGAGGTATTGTACTGATG ATATGCTGCAAAGAGAAATGATGTCCAATCCTTTTTTGGGTAGCTATGGGGTCATCATCTTAGATGATAT TCATGAAAGAAGCATTGCAACTGATGTGTTACTTGGACTTCTTAAAGATGTTTTACTAGCAAGACCAGAA CTGAAGCTCATAATTAACTCCTCACCTCACCTGATCAGCAAACTCAATTCTTATTATGGAAACGTGCCTG TCATAGAAGTGAAAAATAAACACCCTGTGGAGGTTGTGTACCTTAGTGAGGCTCAAAAGGATTCTTTTGA GTCTATTTTACGCCTTATCTTTGAAATTCACCACTCGGGTGAGAAAGGTGACATTGTAGTCTTTCTGGCC TGTGAACAAGATATTGAGAAAGTCTGTGAAACTGTCTATCAAGGATCTAACCTAAACCCAGATCTTGGAG AACTGGTGGTTGTTCCTTTGTATCCAAAAGAGAAATGTTCATTGTTCAAGCCACTCGATGAAACAGAAAA AAGATGCCAAGTTTATCAAAGAAGAGTGGTGTTAACTACTAGCTCTGGAGAGTTTTTGATCTGGAGCAAC TCAGTCAGATTTGTTATCGATGTGGGTGTGGAAAGAAGAAAGGTGTACAACCCGAGAATAAGAGCAAACT CGCTCGTCATGCAGCCCATCAGCCAGAGCCAGGCAGAGATACGCAAGCAGATTCTTGGCTCATCTTCTTC AGGAAAATTTTTCTGCCTGTACACTGAAGAATTTGCCTCCAAAGACATGACGCCACTGAAGCCAGCAGAA ATGCAGGAAGCCAACCTAACAAGCATGGTGCTTTTTATGAAGAGGATAGACATTGCGGGCCTAGGCCACT GTGACTTCATGAACAGACCAGCACCAGAAAGTTTGATGCAGGCATTGGAAGACTTAGATTATCTGGCAGC ACTGGATAATGATGGAAATCTTTCTGAATTTGGAATCATCATGTCAGAGTTTCCTCTTGATCCACAACTC TCGAAGTCTATCTTAGCGTCCTGTGAATTTGACTGTGTAGATGAAGTGCTAACAATCGCGGCCATGGTAA CAGCTCCAAATTGCTTTTCACATGTGCCACATGGAGCTGAAGAGGCTGCCTTGACTTGTTGGAAGACATT TTTACATCCCGAAGGAGATCACTTTACCCTCATCAGCATTTACAAGGCTTACCAAGACACAACTCTGAAT TCTAGCAGTGAGTACTGTGTGGAAAAGTGGTGTCGTGATTACTTCCTCAACTGTTCAGCACTCAGAATGG CAGATGTTATTCGAGCTGAACTCTTAGAAATTATCAAGCGAATCGAGCTTCCCTATGCAGAACCTGCTTT TGGCTCCAAGGAAAACACTCTAAACATAAAGAAAGCTCTTCTGTCCGGTTACTTTATGCAGATTGCTCGG GATGTTGATGGATCAGGTAACTACTTAATGCTGACACATAAGCAGGTTGCTCAGCTGCATCCCCTGTCTG GTTACTCAATCACCAAGAAGATGCCAGAGTGGGTCCTCTTCCATAAATTCAGCATTTCTGAGAACAACTA CATCAGGATTACCTCAGAAATCTCTCCTGAACTATTTATGCAGCTGGTACCACAATACTATTTCAGTAAT CTGCCTCCTAGTGAAAGTAAGGACATTCTACAGCAAGTAGTGGATCACCTATCCCCTGTGTCAACAATGA ATAAGGAACAGCAAATGTGTGAGACGTGCCCTGAAACTGAACAGAGATGCACTCTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209736 protein sequence
Red=Cloning site Green=Tags(s) MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYS FMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGH EVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPE LKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLA CEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSN SVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAE MQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQL SKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLN SSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIAR DVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSN LPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018180 |
| ORF Size | 2229 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018180.3 |
| RefSeq Size | 3070 bp |
| RefSeq ORF | 2232 bp |
| Locus ID | 55760 |
| UniProt ID | Q7L7V1 |
| Domains | HA2 |
| MW | 84.4 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209736L3 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209736L4 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32), mGFP tagged |
CNY 7,700.00 |
|
| RG209736 | DHX32 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32) |
CNY 6,370.00 |
|
| SC310133 | DHX32 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 32 (DHX32) |
CNY 7,220.00 |
