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YTHDC1 (NM_133370) Human Tagged ORF Clone
CAT＃: RC208796
- TrueORF®
YTHDC1 (Myc-DDK-tagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_133370" in other vectors (5)
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CNY 3,990.00
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CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | YT521; YT521-B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208796 ORF sequence, codon optimized.
Due to the complexity of NM_133370, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGATTCAAGAGAGGAAAAAGATGGGGAACTTAACGTTCTGGACGACATACTGACGGAGGTAC CCGAGCAAGACGACGAACTGTACAACCCTGAGTCCGAGCAGGACAAAAATGAAAAGAAGGGGTCCAAAAG GAAGTCTGACAGAATGGAGTCCACCGATACCAAAAGACAGAAGCCTAGTGTGCACTCTAGACAACTGGTA TCCAAGCCCCTGAGCTCATCTGTTTCAAACAATAAAAGAATCGTGTCCACTAAGGGAAAGAGTGCCACCG AGTACAAAAACGAAGAGTATCAGCGGAGTGAGCGGAATAAGCGGCTCGACGCTGACAGAAAGATTAGACT GTCCAGCAGCGCTAGCAGAGAACCTTACAAAAATCAGCCTGAAAAAACATGCGTGAGGAAGCGGGACCCG GAGCGGAGAGCGAAAAGTCCCACCCCAGATGGATCAGAGCGCATAGGCCTCGAGGTTGACAGAAGGGCCT CTCGGAGCTCACAGTCATCCAAAGAGGAAGTCAATTCCGAGGAATACGGGAGCGACCACGAGACTGGTTC CTCCGGCTCCTCTGACGAGCAGGGAAACAACACCGAGAACGAGGAGGAGGGGGTGGAGGAGGACGTTGAG GAAGATGAAGAGGTCGAGGAGGATGCAGAGGAAGACGAAGAAGTGGACGAAGACGGCGAGGAGGAGGAAG AAGAAGAAGAGGAGGAGGAAGAAGAAGAGGAAGAGGAAGAGGAAGAATATGAGCAGGATGAGCGCGACCA GAAGGAAGAAGGCAACGATTACGATACAAGATCAGAAGCATCCGATAGTGGGTCCGAGAGTGTCTCCTTC ACCGACGGCAGCGTGCGCTCCGGTTCTGGCACAGACGGATCTGACGAGAAGAAGAAGGAGCGAAAAAGAG CTCGCGGTATCAGCCCTATCGTGTTTGACCGCTCTGGAAGCAGCGCTAGTGAGAGTTATGCCGACCAGAC CTCAAAGCTGAAGTATGTCCTTCAGGATGCCCGCTTTTTTCTCATTAAGTCTAATAATCATGAAAACGTT TCCCTGGCGAAGGCAAAAGGAGTGTGGTCTACCCTGCCCGTGAATGAGAAAAAACTGAATCTCGCTTTCC GATCCGCCCGCAGCGTCATTCTGATATTCAGCGTTCGGGAGTCAGGTAAATTCCAAGGTTTCGCTAGACT TAGCTCCGAGTCTCACCATGGCGGCAGCCCTATCCATTGGGTGTTGCCCGCTGGAATGTCTGCTAAGATG TTGGGGGGTGTATTTAAGATCGATTGGATTTGCAGGCGGGAGCTCCCATTCACCAAATCAGCACATTTGA CTAATCCATGGAATGAACACAAACCCGTGAAGATAGGCCGGGACGGTCAAGAGATTGAACTGGAATGCGG TACTCAACTGTGCCTTCTGTTCCCTCCAGACGAGTCAATCGATTTGTATCAGGTAATCCACAAGATGAGG CATAAGAGACGAATGCACTCCCAGCCAAGATCCCGAGGTAGGCCTTCTCGAAGAGAACCAGTCAGAGATG TTGGTCGACGGCGCCCGGAGGACTACGACATCCATAACTCTCGGAAGAAGCCTCGCATCGACTACCCCCC CGAATTCCATCAGCGGCCAGGGTATCTGAAAGACCCACGATATCAGGAGGTGGACAGAAGATTCTCAGGT GTACGCCGCGATGTTTTTTTGAACGGAAGCTACAACGACTACGTACGCGAGTTCCACAATATGGGTCCTC CCCCTCCTTGGCAGGGGATGCCACCGTACCCAGGTATGGAACAACCACCACATCATCCCTACTATCAACA CCATGCTCCGCCACCTCAGGCCCATCCTCCATACTCTGGACATCATCCAGTACCTCACGAAGCGAGGTAT CGAGACAAGAGAGTCCACGACTACGACATGAGGGTCGATGACTTTCTCAGAAGGACACAAGCTGTGGTGA GCGGACGAAGAAGTCGGCCACGCGAGCGAGACAGAGAAAGGGAAAGGGACCGCCCACGGGACAACCGCCG GGACCGGGAACGCGACCGGGGTAGGGATAGGGAAAGGGAAAGGGAAAGATTGTGTGATCGGGACCGCGAT AGGGGGGAGCGGGGAAGATACAGGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208796 representing NM_133370
Red=Cloning site Green=Tags(s) MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSDRMESTDTKRQKPSVHSRQLV SKPLSSSVSNNKRIVSTKGKSATEYKNEEYQRSERNKRLDADRKIRLSSSASREPYKNQPEKTCVRKRDP ERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEVNSEEYGSDHETGSSGSSDEQGNNTENEEEGVEEDVE EDEEVEEDAEEDEEVDEDGEEEEEEEEEEEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSF TDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYADQTSKLKYVLQDARFFLIKSNNHENV SLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKM LGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMR HKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSG VRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARY RDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRD RGERGRYRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133370 |
| ORF Size | 2127 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133370.2, NM_133370.3, NP_588611.2 |
| RefSeq Size | 6216 bp |
| RefSeq ORF | 2130 bp |
| Locus ID | 91746 |
| UniProt ID | Q96MU7 |
| Domains | YTH |
| MW | 82.7 kDa |
| Gene Summary | Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552, PubMed:28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). Also recognizes and binds m6A on other RNA molecules (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208796L3 | Lenti-ORF clone of YTHDC1 (Myc-DDK-tagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 2. Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RC208796L4 | Lenti-ORF clone of YTHDC1 (mGFP-tagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 2. Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RG208796 | YTHDC1 (tGFP-tagged) - Human YTH domain containing 1 (YTHDC1), transcript variant 2. Note: ORF is codon optimized |
CNY 4,370.00 |
|
| SC111488 | YTHDC1 (untagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 2 |
CNY 7,220.00 |
|
| SC318191 | YTHDC1 (untagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 2 |
CNY 7,220.00 |
