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HAAO (NM_012205) Human Tagged ORF Clone
CAT＃: RC206273
HAAO (Myc-DDK-tagged)-Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO)
ORF Plasmid: tGFP
"NM_012205" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3-HAO; h3HAO; HAO; VCRL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206273 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCCGCCTGGGAGTGAGGGCCTGGGTGAAGGAGAACCGGGGCTCCTTCCAGCCCCCGGTCTGCA ACAAGCTCATGCACCAGGAGCAGCTCAAAGTCATGTTCATCGGAGGCCCCAACACCAGGAAGGACTATCA CATCGAAGAGGGTGAAGAGGTATTTTACCAGCTGGAGGGAGACATGGTTCTCCGAGTCCTGGAGCAAGGG AAACACCGGGATGTGGTCATTCGGCAGGGAGAGATATTCCTCCTGCCTGCCAGGGTGCCCCACTCACCAC AGAGGTTTGCCAACACCGTGGGGCTGGTGGTTGAGCGAAGGCGGCTGGAGACCGAGCTAGATGGGCTCAG GTACTATGTGGGCGACACCATGGACGTTCTGTTTGAGAAGTGGTTCTACTGCAAGGACCTCGGCACGCAG TTGGCCCCCATCATCCAGGAGTTCTTCAGCTCTGAGCAGTACAGAACAGGAAAGCCCATCCCTGACCAGC TGCTCAAGGAGCCACCATTCCCTCTAAGCACACGATCCATCATGGAGCCCATGTCCCTGGATGCCTGGCT GGACAGCCACCACAGGGAGCTGCAGGCAGGCACACCACTCAGCCTGTTTGGGGACACCTATGAGACCCAG GTGATCGCCTATGGGCAAGGCAGCAGCGAAGGCCTGAGACAGAATGTGGACGTGTGGCTGTGGCAGCTGG AGGGCTCCTCGGTGGTGACAATGGGGGGACGGCGCCTGAGCCTGGCCCCTGATGACAGCCTCCTGGTGCT AGCTGGGACCTCGTATGCCTGGGAGCGAACACAAGGCTCTGTGGCCCTGTCTGTGACCCAGGACCCTGCC TGCAAGAAGCCCCTGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206273 protein sequence
Red=Cloning site Green=Tags(s) MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQG KHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQ LAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQ VIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPA CKKPLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012205 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012205.3 |
| RefSeq Size | 1301 bp |
| RefSeq ORF | 861 bp |
| Locus ID | 23498 |
| UniProt ID | P46952 |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
| MW | 32.6 kDa |
| Gene Summary | 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206273L1 | Lenti ORF clone of Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206273L2 | Lenti ORF clone of Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO), mGFP tagged |
CNY 5,890.00 |
|
| RC206273L3 | Lenti ORF clone of Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206273L4 | Lenti ORF clone of Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO), mGFP tagged |
CNY 5,890.00 |
|
| RG206273 | HAAO (tGFP-tagged) - Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO) |
CNY 4,000.00 |
|
| SC310572 | HAAO (untagged)-Human 3-hydroxyanthranilate 3,4-dioxygenase (HAAO) |
CNY 2,640.00 |
