QPRT (NM_014298) Human Tagged ORF Clone
CAT＃: RC202960
QPRT (Myc-DDK-tagged)-Human quinolinate phosphoribosyltransferase (QPRT)
ORF Plasmid: tGFP
"NM_014298" in other vectors (7)
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CNY 3600.00
| Cited in 1 publication. |
CNY 1999.00
CNY 2700.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-90n; QPRTase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202960 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCTGAAGGCCTGGCGCTGCTGCTGCCGCCCGTCACCCTGGCAGCCCTGGTGGACAGCTGGCTCC GAGAGGACTGCCCAGGGCTCAACTACGCAGCCTTGGTCAGCGGGGCAGGCCCCTCGCAGGCGGCGCTGTG GGCCAAATCCCCTGGGATACTGGCAGGGCAGCCTTTCTTCGATGCCATATTTACCCAACTCAACTGCCAA GTCTCCTGGTTCCTCCCCGAGGGATCGAAGCTGGTGCCGGTGGCCAGAGTGGCCGAGGTCCGGGGCCCTG CCCACTGCCTGCTGCTGGGGGAACGGGTGGCCCTCAACACGCTGGCCCGCTGCAGTGGCATTGCCAGTGC TGCCGCCGCTGCAGTGGAGGCCGCCAGGGGGGCCGGCTGGACTGGGCACGTGGCAGGCACGAGGAAGACC ACGCCAGGCTTCCGGCTGGTGGAGAAGTATGGGCTCCTGGTGGGCGGGGCCGCCTCGCACCGCTACGACC TGGGAGGGCTGGTGATGGTGAAGGATAACCATGTGGTGGCCGCCGGTGGCGTGGAGAAGGCGGTGCGGGC GGCCAGACAGGCGGCTGACTTCGCTCTGAAGGTGGAAGTGGAATGCAGCAGCCTGCAGGAGGCCGTGCAG GCAGCTGAGGCTGGTGCCGACCTTGTCCTGCTGGACAACTTCAAGCCAGAGGAGCTGCACCCCACGGCCA CCGTGCTGAAGGCCCAGTTCCCGAGTGTGGCTGTGGAAGCCAGTGGGGGCATCACCCTGGACAACCTCCC CCAGTTCTGCGGGCCGCACATAGACGTCATCTCCATGGGGATGCTGACCCAGGCGGCCCCAGCCCTTGAT TTCTCCCTCAAGCTGTTTGCCAAAGAGGTGGCTCCAGTGCCCAAAATCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202960 protein sequence
Red=Cloning site Green=Tags(s) MDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGILAGQPFFDAIFTQLNCQ VSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKT TPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQ AAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALD FSLKLFAKEVAPVPKIH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014298 |
| ORF Size | 891 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014298.6 |
| RefSeq Size | 1575 bp |
| RefSeq ORF | 894 bp |
| Locus ID | 23475 |
| UniProt ID | Q15274 |
| Domains | QRPTase |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
| MW | 30.8 kDa |
| Gene Summary | This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Macrophage de novo NAD+ synthesis specifies immune function in aging and inflammation
,Minhas, PS;Liu, L;Moon, PK;Joshi, AU;Dove, C;Mhatre, S;Contrepois, K;Wang, Q;Lee, BA;Coronado, M;Bernstein, D;Snyder, MP;Migaud, M;Majeti, R;Mochly-Rosen, D;Rabinowitz, JD;Andreasson, KI;,
Nat. Immunol.
,PubMed ID 30478397
[QPRT]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202960L1 | Lenti ORF clone of Human quinolinate phosphoribosyltransferase (QPRT), Myc-DDK-tagged |
CNY 6000.00 |
|
| RC202960L2 | Lenti ORF clone of Human quinolinate phosphoribosyltransferase (QPRT), mGFP tagged |
CNY 5890.00 |
|
| RC202960L3 | Lenti ORF clone of Human quinolinate phosphoribosyltransferase (QPRT), Myc-DDK-tagged |
CNY 6000.00 |
|
| RC202960L4 | Lenti ORF clone of Human quinolinate phosphoribosyltransferase (QPRT), mGFP tagged |
CNY 6000.00 |
|
| RG202960 | QPRT (tGFP-tagged) - Human quinolinate phosphoribosyltransferase (QPRT) |
CNY 5200.00 |
|
| SC310560 | QPRT (untagged)-Human quinolinate phosphoribosyltransferase (QPRT) |
CNY 6270.00 |
|
| SC319149 | QPRT (untagged)-Human quinolinate phosphoribosyltransferase (QPRT) |
CNY 3600.00 |
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