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UBA2 (NM_005499) Human Tagged ORF Clone
CAT＃: RC201288
UBA2 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 2 (UBA2)
ORF Plasmid: tGFP
"NM_005499" in other vectors (6)
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CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARX; HRIHFB2115; SAE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201288 representing NM_005499
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGGGGGCCGGGTGCTGGTGGTGG GGGCGGGCGGCATCGGCTGCGAGCTCCTCAAGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGAT TGATCTGGATACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACATGTTGGAAGA TCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTTACCCGAAAGCTAATATCGTTGCCTACCATG ACAGCATCATGAACCCTGACTATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATGTTCCTCTTATTGAAAGTGGA ACAGCTGGGTATCTTGGACAAGTAACTACTATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTA AGCCGACCCAGAGAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATACATTGCATCGT TTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAAGATGCTGATCAAGAAGTATCTCCTGACAGA GCTGACCCTGAAGCTGCCTGGGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGATCCAGTTAAACTTTTTACCAA GCTTTTTAAAGATGACATCAGGTATCTGTTGACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTT CCGTTGGACTGGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAGAATGAACCCC AGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAGCTATGCACGTCTTTTTTCAAAGAGCATCGA GACTTTGAGAGTTCATTTAGCAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAGTATGAATATGAAGAGTAGAT TTGATATCAAATCAATGGCAGGGAACATTATTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTT GATAGTATTGGAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTTTTTGAATAAA CAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCACTGGATCCTCCCAACCCCAATTGTTATGTAT GTGCCAGCAAGCCAGAGGTGACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAGATGGGAAAGGAACAATCCTA ATATCTTCCGAAGAGGGAGAGACGGAAGCTAATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATG GCAGCCGGCTTCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTCATAGTGAAGA CCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCCCCGGAAAAAGTGGGGCCCAAACAAGCTGAA GATGCTGCCAAAAGCATAACCAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC AAGATGACGTTCTCATAGTTGATTCGGATGAAGAAGATTCTTCAAATAATGCCGACGTCAGTGAAGAAGA GAGAAGCCGCAAGAGGAAATTAGATGAGAAAGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAG GAAGAGCTTGATGATGTCATAGCATTAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201288 representing NM_005499
Red=Cloning site Green=Tags(s) MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGR SKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESG TAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDR ADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPV PLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPS AMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNK QPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTIL ISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAE DAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQK EELDDVIALD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005499 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005499.1 |
| RefSeq Size | 2682 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 10054 |
| UniProt ID | Q9UBT2 |
| Domains | UBACT, ThiF |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 71 kDa |
| Gene Summary | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Sumoylation of SAE2 C Terminus Regulates SAE Nuclear Localization
,Khue Truong, Terry D. Lee, Baozong Li, and Yuan Chen,
J. Biol. Chem., Dec 2012; 287: 42611 - 42619.
[UBA2]
|
|
Small Ubiquitin-like Modifier (SUMO) Modification of E1 Cys Domain Inhibits E1 Cys Domain Enzymatic Activity
,Khue Truong, Terry D. Lee, and Yuan Chen,
J. Biol. Chem., May 2012; 287: 15154 - 15163.
[UBA2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201288L1 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 2 (UBA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201288L2 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 2 (UBA2), mGFP tagged |
CNY 5,890.00 |
|
| RC201288L3 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 2 (UBA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201288L4 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 2 (UBA2), mGFP tagged |
CNY 5,890.00 |
|
| RG201288 | UBA2 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 2 (UBA2) |
CNY 4,370.00 |
|
| SC319458 | UBA2 (untagged)-Human ubiquitin-like modifier activating enzyme 2 (UBA2) |
CNY 2,950.00 |
